EVALUATION OF THE CYTOCHROME P450 INHIBITORY EFFECT OF THYME OLEORESIN FROM THYMUS VULGARIS L. - AN IN VITRO STUDYEVALUATION OF THE CYTOCHROME P450 INHIBITORY EFFECT OF THYME OLEORESIN FROM THYMUS VULGARIS L. - AN IN VITRO STUDY
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i9.26759Keywords:
Thyme oleoresin, Cytochrome P450, Inhibitory assay, Thymus vulgarisAbstract
Objective: The objective of this study was to evaluate the effect of thyme oleoresin on cytochrome P450 (CYP3A4) enzyme.
Materials and Methods: The different concentrations of thyme oleoresin (5–100 μg/ml) were examined for its inhibitory property toward cytochrome P450 isoform (CYP3A4). Thyme oleoresin, potassium phosphate buffer, CYP450 reagent, and substrate 7-Benzyloxy-4-trifluoromethylcoumarin were added to a 96-well plate. The mixtures were preincubated for 20 min at room temperature. The fluorescent intensities of the products were measured by PerkinElmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively. Values were expressed as mean ± standard error mean (n=3). IC50 was calculated by plotting concentrations of thyme against the corresponding percent inhibition.
Results: All the tested concentrations of thyme showed inhibitory effect against CYP3A4 in a dose-dependent manner. At 5 μg/ml, it showed a percentage inhibition of 1.82±0.61, whereas 100 μg/ml showed 66.05±0.16. The IC50 value of thyme for CYP3A4 inhibitory activity was found to be 39.14 μg/ml.
Conclusion: This study prove the inhibitory effect of thyme oleoresin on cytochrome P450. The inhibitory effect of thyme oleoresin indicates the possibilities of herb-drug interaction if this extract is co-administered with prescribed drugs that are metabolized by CYP3A4.
Downloads
References
Kemkar K, Sathiyanarayanan L, Sathiyanarayanan A, Mahadik K. 6-Shogaol rich ginger oleoresin loaded mixed micelles enhances in vitro cytotoxicity on MCF-7 cells and in vivo anticancer activity against DAL cells. Int J Pharm Pharm Sci 2018;10:160-8.
Rajalingam D, Varadharajan R, Palani S. Evaluation of hepatoprotective and antioxidant effect of Combretum albidum g. Don against ccl4 induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2016;8:218-23.
Ashwini S, Anitha R. Anti-hyperglycaemic activity of Caralluma fimbriata - An in vitro approach. Pharmacogn Magn 2017;13:S499-544.
Ashwini S, Ezhilarasan D, Anitha R. Cytotoxic effect of Caralluma fimbriata against human colon cancer cells. Pharmacogn J 2017;9:98-101.
Dussault D, Vu KD, Lacroix M. In vitro evaluation of antimicrobial activities of various commercial essential oils, oleoresin and pure compounds against food pathogens and application in ham. Meat Sci 2014;96:514-20.
Mamdapur GM, Hadimani MB. Bibliometric analysis of research output in spices oleoresin during1994–2013: A study with reference to scifinder .Journal of Library Information Communication Technology 2016;1:38-51.
Shaikh J, Bhosale R, Singhal R. Microencapsulation of black pepper oleoresin. Food Chem 2006;94:105-10.
Nam KC, Ko KY, Min BR, Ismail H, Lee EJ, Cordray J, et al. Effects of oleoresin-tocopherol combinations on lipid oxidation, off-odor, and color of irradiated raw and cooked pork patties. Meat Sci 2007;75,61-70.
Upadhyay R, Mishra HN. Antioxidant activity measurement of oleoresin from rosemaryand sage. Ind Crops Prod 2011;61:453-9.
Zancan CK, Marques MO, Petenate AJ, Angela M, Meireles MA. Extraction of ginger (Zingiber officinale Roscoe) oleoresin with CO2 and co-solvents: A study of the antioxidant action of the extracts. J Supercrit Fluids 2002;24:57-76.
Yusop SM, O’sullivan MG, Preuß M, Weber H, Kerry JF, Kerry JP. Assessment of nanoparticle paprika oleoresin on marinating performance and sensory acceptance of poultry meat. Lwt Food Sci Technol 2012;46:349-55.
Kurmudle N, Kagliwal LD, Bankar SB, Singhal RS. Enzyme-assisted extraction for enhanced yields of turmeric oleoresin and its constituents. Food Biosci 2013;3:36-41.
Rodianawati I, Hastuti P, Cahyanto M. Nutmeg’s (Myristica fragrans Houtt) oleoresin: Effect of heating to chemical compositions and antifungal properties. Procedia Food Sci 2015;3:244-54.
Ogu CC, Maxa JL. Drug interactions due to cytochrome P450. Proc (Bayl Univ Med Cent) 2000;13:421-3.
Gavhane YN, Yadav AV. Loss of orally administered drugs in Gi Tract. Saudi Pharm J 2012;20:331-44.
Capello F, Gaddi AV. Springer Clinical Handbook of Air Pollution- Related Diseases. Förlag: Springer International Publishing AG; 2018. p. 215-7.
Bozina N, Bradamante V, Lovric M. Genetic Polymorphism of Cyp Enzymes. Arh Hig Rada Toksikol 2009;60:217-42.
Zanger UM, Schwab M. Cytochrome P450 enzymes in drug metabolism: Regulation of gene expression, enzyme activities, and impact of genetic variation. Pharmacol Ther 2013;138:103-41.
Anannarukan N, Niwattksaiwong N, Warisnoichareon W, Winitthana T, Pramyothin P, Chaichantipyuth C, et al. Inhibition of human cytochrome P450 in vitro by Phyllanthus amarus and Phyllanthus emblica aqueous extracts. Thai J Pharm Sci 2012;36:135-43.
Cheng-Chung T. Identification and localization of five CYP2Cs in murine extrahepatic tissues and their metabolism of arachidonic acid to regio-and stereoselective products. Pharmacol Exp Ther 2001;299:39-47.
Purwantiningsih AH, Chan KL. Effect of standardized Eurycoma longifolia extract on Phase I rosiglitazone metabolism in the old normal male rat hepatocytes: A mechanism study. Int J Pharm Pharm Sci 2015;7:80-3.
Lynch T, Price A. The effect of cytochrome P450 metabolism on drug response, interactions, and adverse effects. Am Fam Physician 2007;76:391-6.
Fujita K. Food-drug interactions via human cytochrome P450 3A (CYP3A). Drug Metabol Drug Interact 2004;20:195-217.
Ponnusankar S, Pandit S, Venkatesh M, Bandyopadhyay A, Mukherjee PK. Cytochrome P450 inhibition assay for standardized extract of Terminalia chebula Retz. Phytother Res 2011;25:151-4.
Modarai M, Gertsch J, Suter A, Heinrich M, Kortenkamp A. Cytochrome P450 inhibitory action of Echinacea preparations differs widely and co-varies with alkylamide content. J Pharm Pharmacol 2007;59:567-73.
Ashour ML, Youssef FS, Gad HA, Wink M. Inhibition of cytochrome P450 (CYP3A4) activity by extracts from 57 plants used in traditional chinese medicine (TCM), Pharmacogn Mag 2017;13:300-8.
Afandi A, Zulkiffli HM, Sadikun A, Ismail S. Antioxidant properties of Gynura procumbens extracts and their inhibitory effects on two major human recombinant cytochrome p450s using a high throughput luminescence assay. Asian J Pharm Clin Res 2014;7:36-41.
Zou L, Harkey MR, Henderson GL. Effects of herbal components on cDNA-expressed cytochrome p450 enzyme catalytic activity. Life Sci 2002;71:1579-89.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
