ESTIMATION OF GUGGULSTERONE-Z IN GOKSHURADI GUGGULU USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Kanan G Gamit; Niraj Y Vyas; Nishit D Patel; Manan A Raval

doi:10.22159/ajpcr.2018.v11i11.26894

Authors

Kanan G Gamit Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India. http://orcid.org/0000-0002-0563-4921
Niraj Y Vyas Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.
Nishit D Patel Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.
Manan A Raval Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.26894

Keywords:

Gokshuradi Guggulu, Guggulsterone-Z, High-performance liquid chromatography

Abstract

Objective: A study was aimed to estimate guggulsterone-Z (GZ) in Gokshuradi Guggulu (GG).

Methods: An analytical method was developed and validated using Waters Alliance high-performance liquid chromatography system (Empower software), equipped with photodiode array detector. Separation was achieved using Phenomenex, C-18 (250 mmÃ—4.6 mm, 5 Î¼) column. Mobile phase consisted of acetonitrile:water (70:30,v/v). Flow rate was set to 1 ml/min and detection was performed at 251 nm.

Results and Discussion: Validation parameters such as linearity, precision, accuracy, limit of detection, limit of quantification, and robustness were performed. Amount of GZ was estimated using linearity equation.

Conclusion: GG was found to contain 0.815Â±0.03 g% w/w GZ. Validated method may be used as one of the parameters to standardize the formulation.

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Author Biographies

Kanan G Gamit, Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.

Department of Pharmacogosy

Niraj Y Vyas, Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.

Department of Pharmacognosy

Nishit D Patel, Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.

Dept of Pharmacognosy

Manan A Raval, Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa, 388421, Anand, Gujarat, India.

Dept of Pharmacognosy

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