ASSESSMENT OF BIOLOGICAL SAFETY OF FERMENTED PHYLLANTHUS EMBLICA FRUIT JUICE
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i9.27104Keywords:
Phyllanthus emblica, Lactic acid bacteria, Fermented plant juice, Subchronic, ToxicityAbstract
Objective: The present study evaluated the subchronic toxicity of Lactobacillus mediated fermented Phyllanthus emblica fruit juice (FPJ) using a rat as a model system.
Methods: FPJ was prepared, and estimated the changes in pH by pH meter, and microbial load by a plating method. Rats were fed with different dose of FPJ for 60 days. The changes in the body mass were noted. The blood and organs of the experimental rats were collected, after 60 days of intervention. Then, they were analyzed for the selected hematological and biochemical parameters by following standard hospital protocols.
Results: The pH of FPJ after 30 days of fermentation was 3.16. FPJ was rich in probiotic Lactobacillus spp. (7.23 Log CFU per mL) without contamination. The supplementation of FPJ was not significantly affected the body weight of the experimental animals, except the female rats in posteffective dose (PED) group showed significant changes (20.83±8.49 g) compared to control (40±17.22 g). The internal organs of the rat were not affected by the FPJ supplementation. The changes observed in blood urea nitrogen, creatinine, cholesterol, triglyceride, aspartate aminotransferase; alanine aminotransferase, and alkaline phosphatase level of experimental rats, both male and female, were not significantly differed from the respective controls. The average of lymphocytes level was significantly increased in continuous dosing group of males and females. Interestingly, the increase in red blood cell and hemoglobin (HGB) were statistically significant for ED group and PED in both sexes, except for females with no effect on HGB content.
Conclusion: The prepared FPJ was enriched with probiotic Lactobacillus spp. The supplementation of FPJ (up to 9 mL/kg/day) for 60 days was not significantly influenced the body weight, internal organs, biochemical and hematological parameters of experimental rats (both male and female). The results revealed that FHJ is suitable for the human consumption.
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Variya BC, Bakrania AK, Patel SS. Emblica officinalis (Amla): A review for its phytochemistry, ethnomedicinal uses and medicinal potentials with respect to molecular mechanisms. Pharmacol Res 2016;111:180-200.
Perianayagam JB, Sharma SK, Joseph A, Christina AJ. Evaluation ofanti-pyretic and analgesic activity of Emblica officinalis Gaertn. J Ethnopharmacol 2004;95:83-5.
Asmawi MZ, Kankaanranta H, Moilanen E, Vapaatalo H. Anti-inflammatory activities of Emblica officinalis gaertn leaf extracts. J Pharm Pharmacol 1993;45:581-4.
Srinivasan K. Plant foods in the management of diabetes mellitus: Spices as beneficial antidiabetic food adjuncts. Int J Food Sci Nutr 2005;56:399-414.
Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;81:81-100.
Akhtar MS, Ramzan A, Ali A, Ahmad M. Effect of Amla fruit (Emblica officinalis Gaertn.) on blood glucose and lipid profile of normal subjects and type 2 diabetic patients. Int J Food Sci Nutr 2011;62:609-16.
Ngamkitidechakul C, Jaijoy K, Hansakul P, Soonthornchareonnon N, Sireeratawong S. Antitumor effects of Phyllanthus emblica L.: Induction of cancer cell apoptosis and inhibition of in vivo tumor promotion and in vitro invasion of human cancer cells. Phytother Res 2010;24:1405-13.
Ojha S, Golechha M, Kumari S, Arya DS. Protective effect of Emblica officinalis (Amla) on isoproterenol-induced cardiotoxicity in rats. Toxicol Ind Health 2012;28:399-411.
Zhang YJ, Nagao T, Tanaka T, Yang CR, Okabe H, Kouno I. Antiproliferative activity of the main constituents from Phyllanthus emblica. Biol Pharm Bull 2004;27:251-5.
Boll M, Weber LW, Becker E, Stampfl A. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z Naturforsch C 2001;56:649-59.
Rahman S, Akbor MM, Howlader A, Jabbar A. Antimicrobial and cytotoxic activity of the alkaloids of Amlaki (Emblica officinalis). Pak J Biol Sci 2009;12:1152-5.
Mehmood MH, Siddiqi HS, Gilani AH. The antidiarrheal and spasmolytic activities of Phyllanthus emblica are mediated through dual blockade of muscarinic receptors and Ca2+channels. J Ethnopharmacol 2011;133:856-65.
Pandaand S, Kar A. Fruit extract of Emblica officinalis ameliorates hyperthyroidism and hepatic lipid peroxidation in mice. Die Pharm 2003;58:753-5.
Sarkhel S, Chakravarty AK, Das R, Gomes A, Gomes A. Snake venom neutralizing factor from the root extract of Emblica officinalis Linn. Orient Pharm Exp Med 2011;11:25-33.
Thirunavukkarasu M, Selvaraju V, Tapias L, Sanchez JA, Palesty JA, Maulik N. Protective effects of Phyllanthus emblica against myocardial ischemia-reperfusion injury: The role of PI3-kinase/glycogen synthase kinase 3beta/beta-catenin pathway. J Physiol Biochem 2015;71:623-33.
Kulkarni AP, Kellaway LA, Kotwal GJ. Herbal complement inhibitors in the treatment of neuroinflammation: Future strategy for neuroprotection. Ann N Y Acad Sci 2005;1056:413-29.
Kumar V. Potential medicinal plants for CNS disorders: An overview. Phytother Res 2006;20:1023-35.
Thakur RS, Ahirwar B. The pharmacological evaluation of medicinal plants for cytotoxicity against various cancer cell lines. Int J Pharm Pharm Sci 2017;9:198-202.
Mehta J, Jandaik S, Urmila S. Evaluation of phytochemicals and synergistic interaction between plant extracts and antibiotics for efflux pump inhibitory activity against Salmonella enterica serovar typhimurium strains. Int J Pharm Pharm Sci 2016;8:217-23.
Sushma KS, Rajeshwari P, Raveesha KA. Comparative study of mycoflora, antibacterial activity and phytochemistry of selected fresh and stored medicinal fruits. Int J Pharm Pharm Sci 2017;9:43-8.
Peerajan S, Chaiyasut C, Sirilun S, Chaiyasut K, Kesika P, Sivamaruthi BS. Enrichment of nutritional value of Phyllanthus emblica fruit juice using the probiotic bacterium, Lactobacillus paracasei HII01 mediated fermentation. Food Sci Technol Campinas 2016;36:116-23.
Woraharn S, Lailerd N, Sivamaruthi BS, Wangcharoen W, Peerajan S, Sirisattha S, et al. Development of fermented Hericium erinaceus juice with high content of L-glutamine and L-glutamic acid. Int J Food Sci Technol 2015;50:2104-12.
Woraharn S, Lailerd N, Sivamaruthi BS, Wangcharoen W, Sirisattha S, Peerajan S, et al. Evaluation of factors that influence the L-glutamic and γ-aminobutyric acid production during Hericium erinaceus fermentation by lactic acid bacteria. Cyta J Food 2016;14:47-54.
Chaiyasut C, Sivamaruthi BS, Makhamrueang N, Peerajan S, Kesika P. A survey of consumer’ opinion about consumption and health benefits of fermented plant beverages in Thailand. Food Sci. Technol Campinas. 2018. [In Press]. DOI: http://dx.doi.org/10.1590/1678-457X.04917.
Woraharn S, Lailerd N, Sivamaruthi BS, Wangcharoen W, Sirisattha S, Chaiyasut C. Screening and kinetics of glutaminase and glutamate decarboxylase producing lactic acid bacteria from fermented Thai foods. Food Sci Technol Campinas 2014;34:793-9.
Komatsuzaki N, Shima J, Kawamoto S, Momose H, Kimura T. Production of γ-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 2005;22:497-504.
Chaiyasut C, Woraharn S, Sivamaruthi BS, Kesika P, Lailerd N, Peerajan S. Lactobacillus fermentum HP3 mediated fermented Hericium erinaceus juice as a health promoting food supplement to manage diabetes mellitus. J Evid Based Integrative Medicine 2018;23:1-9.
Itthipanichpong C, Ousavaplangchai L, Ramart S, Thamaree S, Tankeyoon M. Acute toxicity and subacute toxicity study of Phyllanthus emblica. Chula Med J 1987;31:367-76.
Mokkhasmit M, Swatdimongkol K, Satrawaha P. Study on toxicity of Thai medicinal plants. Bull Dept Med Sci 1971;12:36-65.
Chaiyasut C, Pattananandecha T, Sirilun S, Suwannalert P, Peerajan S, Sivamaruthi BS. Synbiotic preparation with Lactic acid bacteria and inulin as a functional food: In vivo evaluation of microbial activities, and preneoplastic aberrant crypt foci. Food Sci Technol Campinas 2017;37:328-36.
De A, Papasian C, Hentges S, Banerjee S, Haque I, Banerjee SK. Emblica officinalis extract induces autophagy and inhibits human ovarian cancer cell proliferation, angiogenesis, growth of mouse xenograft tumors. PLoS One 2013;8. Article ID: e72748.
Zhu X, Wang J, Ou Y, Han W, Li H. Polyphenol extract of Phyllanthus emblica (PEEP) induces inhibition of cell proliferation and triggers apoptosis in cervical cancer cells. Eur J Med Res 2013;18. Article ID:46.
Shivananjappa MM, Joshi MK. Influence of Emblica officinalis aqueous extract on growth and antioxidant defense system of human hepatoma cell line (HepG2). Pharm Biol 2012;50:497-505.
Ho HH, Chang CS, Ho WC, Liao SY, Wu CH, Wang CJ. Anti-metastasis effects of gallic acid on gastric cancer cells involves inhibition of NF-ðœ…B activity and downregulation of PI3K/AKT/small GTPase signals. Food Chem Toxicol 2010;48:2508-16.
Ho HH, Chang CS, Ho WC, Liao SY, Lin WL, Wang CJ. Gallic acid inhibits gastric cancer cells metastasis and invasive growth via increased expression of RhoB, downregulation of AKT/small GTPase signals and inhibition of NF-kappaB activity. Toxicol Appl Pharmacol 2013;266:76-85.
Golechha M, Sarangal V, Ojha S, Bhatia J, Arya1 DS. Anti-inflammatory effect of Emblica officinalis in rodent models of acute and chronic inflammation: Involvement of possible mechanisms. Int J Inflam 2014;2014. Article ID:178408.
Middha SK, Goyal AK, Lokesh P, Yardi V, Mojamdar L, Keni DS, et al. Toxicological evaluation of Emblica officinalis fruit extract and its anti-inflammatory and free radical scavenging properties. Pharmacogn Mag 2015;11 Suppl 3:S427-33.
Guo X, Wang X. Phyllanthus emblica fruit extract activates spindle assembly checkpoint, prevents mitotic aberrations and genomic instability in human colon epithelial NCM460 cells. Int J Mol Sci 2016;17. Article ID:1437.
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