MORPHOLOGICAL AND HISTOLOGICAL EFFECTS OF BRUCEINE A ON THE LARVAE OF AEDES AEGYPTI LINNAEUS (DIPTERA: CULICIDAE)
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i10.27315Keywords:
Bruceine A, Brucea javanica (L) Merr, Action target, Morphology, Histology, Aedes aegypti LinnaeusAbstract
Objective: This study aimed to determine a target of action of bruceine A on the basis of its morphological and histological effects on the larvae of Aedes aegypti Linnaeus.
Methods: Bruceine A was isolated from Brucea javanica (L.) Merr. seeds in accordance with the Mangungsong method. Larvae of A. Aegypti (L.) in instar III to the beginning of instar IV were treated with various concentrations of bruceine A. The negative control group did not receive any treatment, whereas the positive control group received 1 ppm temefos. Dead larvae were collected after 24 h of treatment for the examination of morphological and histological changes.
Results: The negative control group did not exhibit any morphological and histological changes. Larvae treated with bruceine A, however, had visible damaged heads, cuticles, digestive and respiration tracts, respiratory siphons, and setae, and they were smaller than normal larvae. Larvae treated with temefos exhibited gastrointestinal damage, narrowed breathing tubes, cuticle damage, and detached/damaged seta feathers. The necrosis of gastrointestinal epithelial cells was the major histological change exhibited by larvae treated with various concentrations of bruceine A or 1 ppm temefos.
Conclusion: The targets of action of bruceine A in A. aegypti (L.) larvae are the head/caput, cuticle, setae, siphon, and gastrointestinal and respiratory tracts.
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Morrison AC, Zielinski-Gutierrez E, Scott TW, Rosenberg R. Defining challenges and proposing solutions for control of the virus vector Aedes aegypti. PLoS Med 2008;5:e68.
Ponlawat A, Scott JG, Harrington LC. Insecticide susceptibility of Aedes aegypti and Aedes albopictus across thailand. J Med Entomol 2005;42:821-5.
Jansen CC, Beebe N. The dengue vector of Aedes aegypti : What comes next. Microbes Infect 2010;12:272-9.
Sanchez L, Vanlerberghe V, Alfonso L, Marquetti Mdel C, Guzman MG, Bisset J, et al. Aedes aegypti larval indices and risk for dengue epidemics. Emerg Infect Dis 2006;12:800-6.
El-Akhal F, Guemmouh R, Maniar S, Taghzouti K, El Quali Lalami A. Larvicidal activity of essential oils of Thymus vulgaris and Origanum majorana (Lamiaceae) against of the Malaria vector Anopheles labranchiae (Diptera: Culicidae). Int J Pharm Pharm Sci 2016;8:372-6.
Hirota BC, De Oliveira CD, Merino FJ, Verdam MC, Da Silva CB, Murakami FS, et al. Larvicide and antifungal activities of Sarsaparilla (Smilax larvata) extracts. Int J Pharm Pharm Sci 2015;7:308-11.
Zhang L, Feng X, Ma D, Yang J, Jiang H, Zhang Y, et al. Brusatol isolated from Brucea javanica (L.) merr. Induces apoptotic death of insect cell lines. Pestic Biochem Physiol 2013;107:18-24.
Sutiningsih D, Nurjazuli. Effect of brusatol biolarvicide administration on behavioral response of Aedes aegypti and its toxicity on vero cells. J Biol Sci 2017;17:127-35.
Bawm S, Matsuura H, Elkhateeb A, Nabeta K, Subeki, Nonaka N, et al. In vitro antitrypanosomal activities of quassinoid compounds from the fruits of a medicinal plant, Brucea javanica. Vet Parasitol 2008;158:288-94.
Pan L, Chin YW, Chai HB, Ninh TN, Soejarto DD, Kinghorn AD, et al. Bioactivity-guided isolation of cytotoxic constituents of Brucea javanica collected in vietnam. Bioorg Med Chem 2009;17:2219-24.
Elkhateeb A, Yamasaki M, Maede Y, Katakura K, Nabeta K, Matsuura H. Anti-babesial quassinoids from the fruits of Brucea javanica. Nat Prod Commun 2008;3:145-8.
Subeki, Matsuura H, Takahashi K, Nabeta K, Yamasaki M, Maede Y, et al. Screening of indonesian medicinal plant extracts for antibabesial activity and isolation of new quassinoids from Brucea javanica. J Nat Prod 2007;70:1654-7.
Klocke J, Arisawa M, Handa S, Kinghorn A, Cordel I, Farnsworth N. Growth inhibitory, insecticidal and antifeedant effects of some antileukemic and cytotoxic quassinoids on two species of agricultural pest. Chem Org Naturst 1985;47:222-64.
Leskinen V, Polonsky J, Bhatnagar S. Antifeedant activity of quassinoids. J Chem Ecol 1984;10:1497-507.
Sutiningsih D, Mustofa, Satoto TB, Martono E. Neurotoxic mechanism of bruceine A biolarvicide against Aedes aegypti linnaeus larvae. Res J Med Plants 2017;11:77-85.
Sutiningsih D, Mustofa, Satoto TB, Martono E. Inhibitory effects of bruceine A biolarvicide on growth and development of Aedes aegypti larvae. J Entomol 2017;14:104-11.
Mangungsong S. The Activity of Semisynthetic Quassinoid Compound from Makassar Fruit (Brucea javanica L. Merr) as Anticancer with Target Protein p53, Bcl-2, Caspase- 3, COX-2, and c-Myc. Ph. D Thesis. Faculty of Medicine, Gadjah Mada University, Yogyakarta; 2012.
Sharma A, Kumar S, Tripathi P. Impact of Achyranthes aspera leaf and stem extracts on the survival, morphology, and behaviour of an Indian strain of dengue vector, Aedes aegypti. J Mosq Res 2015;5:1-9.
Narciso JO, Soares RO, Reis dos Santos Mallet J, Guimarães AÉ, de Oliveira Chaves MC, Barbosa-Filho JM, et al. Burchellin: Study of bioactivity against aedes aegypti. Parasit Vectors 2014;7:172.
Bogoriani N. Isolation and identification steroid glycosides from andong leaves (Cordyline terminalis Kunth). J Chemistry 2008;2:40-4.
Barbosa LF, Braz-Filho R, Vieira IJ. Chemical constituents of plants from the genus simaba (Simaroubaceae). Chem Biodivers 2011;8:2163-78.
Warikoo R, Kumar S. Impact of Argemone mexicana extracts on the cidal, morphological, and behavioral response of dengue vector, Aedes aegypti L. (Diptera: Culicidae). Parasitol Res 2013;112:3477-84.
Insun D, Choochote W, Jitpakdi A, Chaithong U, Tippawangkosol P, Pitasawat B, et al. Possible site of action of Kaempferia galanga in killing Culex quinquefasciatus larvae. Southeast Asian J Trop Med Public Health 1999;30:195-9.
Chaithong U, Choochote W, Kamsuk K, Jitpakdi A, Tippawangkosol P, Chaiyasit D, et al. Larvicidal effect of pepper plants on aedes aegypti (L.) (Diptera: Culicidae). J Vector Ecol 2006;31:138-44.
Chen YY, Pan QD, Li DP, Liu JL, Wen YX, Huang YL, et al. New pregnane glycosides from Brucea javanica and their antifeedant activity. Chem Biodivers 2011;8:460-6.
Dono D, Ismayana S, Idar, Prijono D, Muslikha I. Status and biochemical resistance of Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) to organophosphate insecticide and its sensitivity to botanical insecticide. Indo J Entomol 2010;7:9-27.
Isman MB. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol 2006;51:45-66.
Kringer R. Handbook of Pesticide Toxicology. California: Academic Press; 2010.
Lu FC, Kacew S. Lu’s Basic Toxicology: Fundamentals, Targets Organ and Risk Assessment. 4th ed. New York: Taylor and Francis; 2002.
Herms WB. Medical Entomology. 6th ed. New York: Macmillan; 1969.
Alves IA, Miranda HM, Soares LA, Randau KP. Simaroubaceae family: Botany, chemical composition, and biological activities. Rev Bras Farmacogn 2014;24:481-501.
Wulandari S, Arnetis, Rahayu S. Potential of sap papaya fruit (Carica papaya L) against mortality of Aedes albopictus mosquitoes larvae. Biogenesis 2012;9:69-75.
Yulidar, Hadifah Z. The abnormalities of larvae’s morphology after temephos exposure in phase larvae instar 3 (L3). J Buski 2014;5:23-8.
Badyaev AV. Stress-induced variation in evolution: From behavioural plasticity to genetic assimilation. Proc Biol Sci 2005;272:877-86.
Thavara U, Tawatsin A, Srithommarat R, Zaim M, Mulla MS. Sequential release and residual activity of temephos applied as sand granules to water-storage jars for the control of Aedes aegypti larvae (Diptera: Culicidae). J Vector Ecol 2005;30:62-72.
Chen CD, Lee HL. Laboratory bioefficacy of CREEK 1.0G (temephos) against aedes (Stegomyia) aegypti (Linnaeus) larvae. Trop Biomed 2006;23:220-3.
Matsumura F. Toxicology of Insecticides. 2nd ed. New York: Plenum Press; 1985.
Yu S. The Toxicology and Biochemistry of Insecticides. Boca Raton: CRC Press; 2008.
Patil PB, Kallapur SV, Kallapur VL, Holihosur SN. Larvicidal activity of Clerodendron inerme gaertn extracts against Aedes aegypti L. and Culex quinquefasciatus Say. Mosquito species. Asian J Pharm Clin Res 2014;7 Suppl 1:206-9.
Jordan TV, Shike H, Boulo V, Cedeno V, Fang Q, Davis BS, et al. Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in Dipteran insects. Insect Mol Biol 1998;7:215-22.
Tellam RL, Wijffels G, Willadsen P. Peritrophic matrix proteins. Insect Biochem Mol Biol 1999;29:87-101.
Moser B, Becnel J, White S, Alfonso C, Kutish G, Shanker S, et al. Morphological and molecular evidence that Culex nigripalpus baculovirus is an unusual member of the family baculoviridae. J Gen Virol 2001;82:283-97.
Silva VC, Pinheiro NL, Scherer PO, Falcão SS, Ribeiro VR, Mendes RM, et al. Histology and ultrastructure of Aedes albopictus larval midgut infected with Bacillus thuringiensis var. Israelensis. Microsc Res Tech 2008;71:663-8.
Al-Mehmadi R, Al-Khalaf A. Larvicidal and histological effects of Melia azedarach extract on Culex quinquefasciatus say larvae (Diptera: Culicidae). J King Saud Univ 2010;22:77-85.
Assar AA, el-Sobky MM. Biological and histopathological studies of some plant extracts on larvae of Culex pipiens (Diptera: Culicidae). J Egypt Soc Parasitol 2003;33:189-200.
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