IN VITRO ANTI-INFLAMMATORY, ANTIOXIDANT AND NEPHROPROTECTIVE STUDIES ON LEAVES OF AEGLE MARMELOS AND OCIMUM SANCTUM

Abstract

Objective: To study the in vitro anti-inflammatory, antioxidant and nephroprotective studies on leaves of Aegle marmelos and Ocimum sanctum.

Methods: In vitro nephroprotective screening of aqueous extract of leaves were performed employing cytoprotective assay using epiflourescence dual staining assay was performed against normal kidney cell treated with gentamicin. In vitro anti-inflammatory studies were also performed for the extracts using protease inhibition activity, membrane stabilization and protein denaturation inhibition assays. In vitro nephroprotective activity was conducted using gentamicin induced normal rat kidney cells. In silico modelling and docking studies were also performed and in vitro cytoprotective assay was performed to evaluate cell cytotoxic efficacy.

 Results: Leaf extracts clearly revealed the dose dependent increase in nephroprotection. Similarly in vitro antioxidant and anti-inflammatory activities proved scientifically the ethnomedicinal claim of these selected plant drugs. Aegelin, marmin and marmesin identified from Aegle marmelos extract showed good binding affinity towards the active site of MCP1 and NGAL protein. In silico modelling and docking studies were also performed to suggest the probable mechanism of action of these plant extracts. In vitro cytoprotective assay showed its potent cell cytotoxic efficacy.

Conclusion: The present study showed in vitro antioxidant and anti-inflammatory activities along with nephroprotective property which scientifically prove the ethnomedicinal claim of these selected plant drugs.

Keywords: Aegle marmelos, Ocimum sanctum, antioxidant, anti-inflammatory, cytoprotective, nephroprotective, in silico, ethnomedicinal.