VERNONIA CINEREA (NEICHITTI KEERAI) REGENERATES PROXIMAL TUBULES IN CISPLATININDUCED RENAL DAMAGE IN MICE

Arul Amuthan; Vasudha Devi; Chandrashekara Shastry Shreedhara; Venkata Rao; Kunal Puri; Shiny Jasphin

doi:10.22159/ajpcr.2019.v12i1.27464

Authors

Arul Amuthan Department of Pharmacology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Vasudha Devi Department of Pharmacology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Chandrashekara Shastry Shreedhara Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Venkata Rao Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Kunal Puri Department of Hematology, Medical College, Kolkata, India.
Shiny Jasphin National Post-Doctoral Fellowship (SERB), Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka State, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.27464

Keywords:

Siddha, Ayurveda, Nephrotoxicity, Renal tubular damage, Tubular cells, Regeneration

Abstract

Objective: The aim of the study was to evaluate whether Vernonia cinerea (VC) regenerates the proximal renal tubular cells in cisplatin-induced necrosis in male Swiss albino mice.

Methods: The crude aqueous extract (CAE) of VC was fractionated from non-polar to polar using different solvents. Mice were injected a single dose of cisplatin (15 mg/kg) on day 1, which took 5 days to cause maximal renal damage. From day 6, CAE and all fractions were orally administered (200, 300, and 400 mg/kg) for 5 continuous days. On day 11, blood was collected to estimate urea and creatinine. Kidney was collected for histology and grading was done.

Results: Cisplatin induced proximal renal tubular damage (grade 5) in corticomedullary junction, characterized by necrosis, proximal tubular dilatation, inflammation and vasodilation. Aqueous fraction (AF) did not show any regeneration; whereas, 400 mg/kg dose of CAE and butanol fraction (BF) showed a significant reduction (p<0.001) in proximal tubular damage (Grade 3) and 50â€“75% regeneration of proximal tubular epithelial cells.

Conclusion: This is the first study to demonstrate the regenerative potential of Neichitti kashayam (CAE of VC) and its BF in cisplatin-induced proximal tubular damage in kidney. Further study is warranted to find out the dose regimen for complete regeneration, lead compounds, and molecular mechanism.

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Author Biographies

Arul Amuthan, Department of Pharmacology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.

Senior Grade Lecturer, Department of Pharmacology

Vasudha Devi, Department of Pharmacology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.

Professor and Head,
Department of Pharmacology

Chandrashekara Shastry Shreedhara, Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.

Professor and Head,

Department of Pharmaceutical Biotechnology

Venkata Rao, Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.

Professor and Head,

Kunal Puri, Department of Hematology, Medical College, Kolkata, India.

Senior Resident, Department of hematology

Shiny Jasphin, National Post-Doctoral Fellowship (SERB), Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka State, India.

National Post-Doctoral Fellowship (SERB), Melaka Manipal Medical College

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