EVALUATION AND CHARACTERIZATION OF BIOACTIVE COMPOUND OF HAMELIA PATENS PLANT WITH THE GC-MS SPECTROSCOPY

Jafra Bano; Swapna Santra; Ekta Menghani

doi:10.22159/ajpcr.2018.v11i11.27557

Authors

Jafra Bano Department of Biotechnology, JECRC University, Jaipur - 303 905, Rajasthan, India.
Swapna Santra Department of Biotechnology, JECRC University, Jaipur - 303 905, Rajasthan, India.
Ekta Menghani Department of Biotechnology, JECRC University, Jaipur - 303 905, Rajasthan, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.27557

Keywords:

Gas chromatography, Methanol extract, Bioactive, Therapeutic agent, National Institute of Standards And Technology, Willey

Abstract

Objective: The aim of this study is to evaluate the bioactive compound present in the leaves, root, flower, and stem by gas chromatography (GC) analysis. Novel drugs are isolated from plants against human diseases.

Methods: The plant parts (leaves, stem, flower, and root) were collected, washed, shade dried, and powdered, and the methanol extracts of all plant parts were prepared by Soxhlet reflux method. The methanolic extracts were analyzed for the identification of phytochemical compounds present in the Hamelia patens plant parts using GC-mass spectrometry (MS) matched by the National Institute of Standards And Technology-11 library and Willey 8 library.

Results: The bioactive fraction on GC-MS analysis revealed a chromatogram showing highest peaks. Methanol extracts of H. patens leaf are found to possess a wide range of saturated and unsaturated aliphatic hydrocarbons and their isomers such as 2,3-dihydro-3,5-dihydroxy-6-methyl- 4H-pyran (1.77%), 1,3-propanediol, 2-ethyl-2-(hydroxymethyl) (3.06%), mome inositol (18.22%), pentadecanoic acid (1.66%), and squalene (11.47%). GC-MS analysis revealed chromatogram, showing that the highest peaks of H. patens stem are methyl salicylate (3.41%), 2-amino-9-(3,4- dihydroxy-5- hydroxymethyl) (9.53%), mome inositol (63.73%), and squalene (1.07%).

Conclusion: Isolation of such bioactives and their use as therapeutic target is the prime motto of our research to isolate novel bioactive that can used as potential therapeutic agents.

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Author Biography

Jafra Bano, Department of Biotechnology, JECRC University, Jaipur - 303 905, Rajasthan, India.

Department of science, JECRC University,

Rank- Research scholar

References

Sathya A, Ramasubramaniaraja R, Brindha P. Pharmacognostical, phytochemical and GC-MS investigation of successive extract of Gymnema sylvestre R. Br. J Pharm Res 2010;3:984-7.

Edeoga HO, Okwu DE, Baebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005;4:685-8.

Hites RA. Gas chromatography and mass spectrometry. Ch. 31. In: Settle B, editor. Handbook of Instrumental Techniques for Analytical Chemistry. NJ, USA: Prentice Hall Publishers; 1997. p. 609-26.

Wagner H, Ulrich-Merzenich G. Synergy research: Approaching a new generation of phytopharmaceuticals. Phytomedicine 2009;16:97 110.

Hirt HM, Pia BM. Natural Medicine in the Tropics I: Foundation Text. Winnenden: Anamed; 2008. p. 124-56.

Elizabeth GE, Aneesh TP, Thomas DG, Anandan R. GC-MS analysis of phytochemical compounds present in the rhizomes of Nervilia aragoana. Asian J Pharm Clin Res 2013;6:68-74.

Kumar SS, Samydurai P, Ramakrishnan R Nagarajan N. Gas chromatography and mass spectrometry analysis of bioactive constituents of Adiantum capillus-veneris. Int J Pharm Pharm Sci 2014;6:60-3.

Balaji K, Kilimozhi D. GC-MS analysis of various extracts of Clerodendrum phlomidis leaf. Int J Pharm Pharm Sci 2013;6:226-32.

Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, et al. Comparative antioxidant activity of individual herbal components used in ayurvedic medicine. Phytochemistry 2003;63:97-104.

Sumathi P, Parvathi A. Antimicrobial activity of some traditional medicinal plants. J Med Plant Res 2010;4:316-21.

Zaidan MR, Noor Rain A, Badrul AR, Adlin A, Norazah A, Zakiah I, et al. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed 2005;22:165-70.

Duke JA, Beckstrom-Sternberg SM. Phytochemical and Ethnobotanical Databases, Dukeâ€™s Phytochemical and Ethnobotanical Databases. Available from: http://www.huilesutiles.eu/docs/patchouli.pdf. [Last cited on 2013 Mar 11].

Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italic leaf methanol extract, Asian J Pharm Clin Res 2012;5:90-4.