INVESTIGATION ON IN VIVO ANALGESIC ACTIVITY OF METHANOL EXTRACT OF MARINE BROWN ALGA SPATOGLOSSUM ASPERUM J. AGARTH
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i11.27760Keywords:
Acetic acid writhing, Analgesic, Methanol extract, Spatoglossum asperumAbstract
Objective: In the present study, evaluation of the methanol extract of Spatoglossum asperum, brown marine algae on the analgesic activity using animal model was focussed.
Methods: Acetic acid-induced writhing test on Wistar albino rats at a dosage of 10 mg/kg body weight of algal extract have been carried out. It showed significant analgesic activity by reducing the number of acetic acid-induced writhing.
Results: The animals at a dosage of 10 mg/kg body weight exhibited, 70.52% of the animals were protected using S. asperum extract, on the other hand, the standard, diclofenac protected 84.21% of the animals. The results are statistically significant at p<0.001, and the investigation revealed dose-dependent significant activity in comparison with standard and control.
Conclusion: Hence, it can be concluded that the methanol extracts of the brown alga S. asperum have potent analgesic activity at moderate doses.
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References
Harper D. Online Etymology Dictionary: Analgesia. 2001, Available from: https://www.etymonline.com/word/analgesia. [Last accessed on 2012 Dec 03].
Dworkin RH, Corbin AE, Young JP. Pregabalin for the treatment of postherpetic neuralgia: A randomized, placebo-controlled trial. Neurology 2003;60:1274-83.
Ghori SS, Ahmed MI, Ariffudin MD, Khateeb S. Evaluation of analgesic and anti-inflammatory activities of formulation containing camphor, menthol and thymol. Int J Pharm Pharm Sci 2016;8:271-4.
Adebisi IM, Abubakar A, Abubakar K, Giaze TR. Analgesic effect and anti-inflammatory activity of aqueous extract Of Boswellia Dalzielii (Burseraceae) Stem Bark. Int J Pharm Pharm Sci 2018;10:1-4.
Anca JM, Lamela M, Calleja JM. Activity on the central nervous system of Himanthalia elongata. Planta Med 1993;59:218-20.
Guzmán S, Gato A, Calleja JM. Antiinflammatory, analgesic and free radical scavenging activities of the marine microalgae Chlorella stigmatophora and Phaeodactylum tricornutum. Phytother Res 2001;15:224-30.
Viana GS, Freitas AL, Lima MM, Vieira LA, Andrade MC, Benevides NM. Antinociceptive activity of sulfated carbohydrates from the red algae Bryothamnion seaforthii (Turner) Kutz. And B. triquetrum (SG Gmel) M. Howe. Braz J Med Biol Res 2002;35:713-22.
Genovese G, Tedone L, Hamann MT, Morabito M. The mediterranean red alga Asparagopsis: A source of compounds against Leishmania. Mar Drugs 2009;7:361-6.
Thennarasan S, Sivamurugan V, Murugesan S, Chidambaranathan N. Analgesic, anti-inflammatory and antipyretic activity of the methanol extracts of brown alga Lobophora variegata (J.V.Lamouroux) womersley ex E.C.oliveir. Am J Phytomed Clin Ther 2016;4:42-57.
Blunt I, Bardsley M, Dixon J. Trends in Emergency Admissions in England 2004 -2009: Is Greater Efficiency Breeding Inefficiency. London: Nuffield Trust; 2010.
Rangaiah GS, Lakshmi P, Sruthikeerthi K. Antibacterial activity of the crude extracts of Chlorophycean seaweeds Ulva, Caulerpa and Spongomorpha sps. against clinical and phytopathogens. Drug Inv Today 2010;2:311-4.
Srivastava N, Saurav K, Mohanasrinivasan V, Kannabiran K, Singh M. Antibacterial potential of macroalgae collected from the Madappam coast, India. Br J Pharm Toxicol 2010;1:72-6.
Lavanya R, Veerappan N. Antibacterial potential of six seaweeds collected from gulf of mannar of southeast coast of India. Adv Biol Res 2011;5:38-44.
Kayalvizhi K, Vasuki S, Anantharaman P, Kathiresan K. Antibacterial activity of seaweeds from the Gulf of Mannar. Int J Pharm Appl 2012;3:306-14.
Pandithurai M, Murugesan S, Sivamurugan V. Antibacterial activity of various solvent extracts of marine brown alga Spatoglossum asperum. Int J Pharmacol Res 2015;5:133-8.
Murugesan S, Bhuvaneswari S, Sivamurugan V. Green synthesis, characterization of silver nanoparticles of a marine red alga Spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;9:1-6.
Hsu JL, Ruoss SJ, Bower, ND, Lin M, Holodniy M, Stevens DA. Diagnosing invasive fungal disease in critically ill patients. Crit Rev Microbiol 2011;37:277-312.
Di Santo R. Natural products as antifungal agents against clinically relevant pathogens. Nat Prod Rep 2010;27:1084-98.
Brown GD, Denning DW, Levitz SM. Tackling human fungal infections. Science 2012;336:647.
Tani N, Rahnasto-Rilla M, Wittekindt C. Antifungal activities of novel non-azole molecules against S. cerevisiae and C. albicans. Eur J Med Chem 2012;47:270-7.
Pandithurai M, Murugesan S, Sivamurugan V, Selvan NT. Antifungal activity of various solvent extracts of marine brown alga Spatoglossum asperum. Int J Pharm Chem 2015;5:278-80.
Thennarasan S, Murugesan S, Sivamurugan V. Green synthesis of silver nano particles using marine brown alga Lobophora variegata and its efficacy in antifungal activity. World J Pharm Res 2015;4:2137-45.
RodrÃguez II, Shi YP, Garcia OJ, Rodriguez AD, Mayer AM, Sanchez JA, et al. New pseudopterosin and secopseudopterosin diterpene glycosides from two colombian isolates of Pseudopterogorgia elisabethae and their diverse biological activities. J Nat Prod 2004;67:1672-80.
Pandithurai M, Murugesan S. Free radical scavenging activity of methanolic extract of brown alga Spatoglossum asperum. J Chem Pharm Res 2014;6:128-32.
Thennarasan S, Murugesan S, Sivamurugan V. Structural identification and anti-oxidant properties of methanolic extract of brown alga Lobophora variegata (J. V. Lamouroux) womersley ex E.C.oliveir. IJIPBART 2015;2:165-78.
Murugesan S, Bhuvaneswari S, Selvam NT. Evaluation of antioxidant property of methanolic extract of red algae Chondrococcus hornemannii and Spyridia fusiformis. J Chem Pharm Res 2015;7:333-7.
Kiran MV, Murugesan S. Biogenic silver nanoparticles by Halymenia poryphyroides and its in vitro anti-diabetic efficacy. J Chem Pharm Res 2013;5:1001-8.
Murugesan S, Babu MA, Bhuvaneswari S, Kotteswari M, Thennarasan S. In vitro antidiabetic activity of methanolic extracts of selected marine algae. Eur J Pharm Med Res 2015;2:256-60.
Pandithurai M, Murugesan S, Bhuvaneswari S, Thennarasan S. In vitro α-amylase and α-glucosidase inhibition activity of methanolic extract of marine brown alga Spatoglossum asperum. Int J Adv Pharm 2015;4:83-7.
Krishnamurthy V, Baluswami M. Phaeophyceae of India and Neighbourhood. Ectocarpales, Sphacelariales, Dictyotales, Chordariales and Scytosiphonales. pp. [2], i-iv, 1-193, 308 figs, pls I-VI. Vol. I. Chennai: Krishnamurthy Institute of Algology; 2010.
Koster R, Anderson M, De Beer E. Acetic acid for analgesic screening. Fed Proc 1959;18:412-8.
Williamson EM, Okpako DT, Evans FJ. Pharmacological Methods in Phytotherapy Research: Selection, Preparation and Pharmacological Evaluation of Plant Material. New York: John Wiley and Sons; 1996. p. 1-23.
GarcÃa MD, Fernández MA, Alvarez A, Saenz MT. Antinociceptive and anti-inflammatory effect of the aqueous extract from leaves of Pimenta racemosa var. ozua (Mirtaceae). J Ethnopharmacol 2004;91:69-73.
Dar A, Baig HS, Saifullah SM, Ahmad VS, Yasmeen S, Nizamuddin M. Effect of seasonal variation on the anti-inflammatory activity of Sargassum wightii growing on the N. Arabian Sea coast of Pakistan. J Exp Mar Biol Ecol 2007;351:1-9.
Silva ED, Scheuer PJ. Manoalide, an antibiotic sesterterpenoid from the marine sponge Luffariella variabilis (Poleajaeff). Tetrahedron Lett 1980;21:1611-4.
Chong AS, Parish CR. Non-immune lymphocyte macrophage interaction II. Evidence that the interaction involves sulfated polysaccharide recognition. Cell Immunol 1985;92:277-89.
Gil B, Ferrándiz ML, Sanz MJ, Terencio MC, Ubeda A, Rovirosa J, et al. Inhibition of inflammatory responses by epitaondiol and other marine natural products. Life Sci 1995;57:25-30.
Awad NE. Biologically active steroid from the green alga Ulva lactuca. Phytother Res 2000;14:641-3.
Lucas R, Giannini C, Dauria MV, Payá M. Modulatory effect of bolinaquinone, a marine sesquiterpenoid, on acute and chronic inflammatory processes. J Pharmacol Exp Ther 2003;304:1172-80.
Jung W, Choi I, Oh S, Park SW, Seo SK, Lee SW, et al. Anti-asthmatic effect of marine red alga (Laurencia undulata) polyphenolic extracts in a murine model of asthma. Food Chem Toxicol 2009;47:293-7.
Payá M, Ferrándiz ML, Sanz MJ, Bustos G, Blasco R, Rios JL, et al. Study of the antioedema activity of some seaweed and sponge extracts from the mediterranean coast in mice. Phytother Res 1993;7:159-62.
Ming K, Jing W, Hongwen H. Demographic bottlenecks and low gene flow in remnant populations of the critically endangered Berchemiella wilsonii var. pubipetiolata (Rhamnaceae) inferred from microsatellite markers. Conserv Genet 2008;9:191-9.
James LR, McIntyre MD. Conditional Reasoning Test of Aggression Test Manual. Knoxville, TN: Innovative Assessment Technology; 2000.
James LR, McIntyre MD, Glisson CA, Bowler J, Mitchell TR. The conditional reasoning measurement system for aggression: An overview. Hum Perform 2004;17:271-95.
Turner RA. In: Turner R, Ebborn P, editors. Analgesics: Screening Methods in Pharmacology. New York: Academic Press; 1965. p. 100.
Bisgaard H. Role of leukotrienes in asthma pathophysiology. Ped Pulmo 2000;30:166-76.
Bley KR, Hunter JC, Eglen RM, Smith JA. The role of IP prostanoid receptors in inflammatory pain. Trends Pharmacol Sci 1998;19:141-7.
Carte BK. Biomedical potential of marine natural products. Bioscience 1996;46:271-86.
Staats PS, Yearwood T, Charapata SG, Presley RW, Wallace MS, Byas-smith M, et al. Intrathecal ziconotide in the treatment of refractory pain in patients with cancer or AIDS-a randomized controlled trial. J Am Med Assoc 2004;291:63-70.
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