A study on phytochemical properties and antioxidant activity of different solvent extract of Siddha drug Karisalai Karpam chooranam in invitro condition

Authors

  • BHARATHKUMAR G The Tamilnadu Dr. MGR. Medical UniversityAnnasalai, Guindy Chennai-32
  • PITCHIAH KUMAR M

Abstract

In Siddha system of medicine drugs consist of polyherbal formulations, containing different plants, used for the treatment of various diseases. Karisalai Karpam chooranam is a polyherbal formulation consisting of seven traditionally used herbs since there is no information about pharmaceutical activities of antioxidant and phytochemical evaluation, the present study focused on the in vitro antioxidant methods where different solvent extract of Karisalai Karpam chooranam screened for ABTS free radical scavenging activity, lipid peroxidation assay, superoxide scavenging activity, metal chelating assay, nitric oxide scavenging activity, and Antioxidant activity in hemoglobin induced linoleic acid. The results revealed potent scavenging activity when compared with standard. The aqueous-methanol extract of Karisalai Karpam chooranam was screened for further phytochemicals which indicated the presence of alkaloids, flavonoids, tannin, saponin, tepenoids, polyphenols and absence of glycosides, anthocyanin. These metabolites profile was recorded with thin layer chromatography. The result indicated promising antioxidant activity of crude extract and needs further exploration for their effective use in both modern and traditional system of medicines.

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Author Biography

BHARATHKUMAR G, The Tamilnadu Dr. MGR. Medical UniversityAnnasalai, Guindy Chennai-32

Department of Siddha

References

Dina A, Nassima C, Meriem B, Karima A, Hakima L, Hania B. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem 2009;112:303-9.

Hancock JT, Desikan R, Neill SJ. Role of reactive oxygen species in cell signalling pathways. Biochem Soc Trans 2001;29(Pt 2):345-50.

Bagchi D, Bagchi M, Stohs SJ, Das DK, Ray SD, Kuszynski CA, et al. Free radicals and grape seed proanthocyanidin extract: Importance in human health and disease prevention. Toxicology 2000;148(2 3):187 97.

Klimczak I, Malecka M, Szlachta M, Gliszczynska A. Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. J Food Compos Anal 2007;20:313-22.

Allen ST. Chemical Analysis of Ecological Material. New York: Blackwell Scientific Publication; 1974. p. 313.

Harbone JR. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. London: Charpan and Hall; 1976. p. 78.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.

Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971;44(1):276 87.

Tripathi YB, Pandey E. Role of alcoholic extract of shoot of Hypericum perforatum Linn on lipid peroxidation and various species of free radicals in rats. Indian J Exp Biol 1999;37(6):567-71.

Tripathi YB, Sharma M, Upadhyay BN, Kumar DS. Antioxidant properties of Rubia cordifoli. Br J Phytotherapy 1998;4(4):163-7.

Bishayee S, Balasubramanian AS. Lipid peroxide formation in rat brain. J Neurochem 1971;18(6):909-20.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351 8.

Gülçin I, Büyükokuroglu ME, Oktay M, Küfrevioglu OI. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. J Ethnopharmacol 2003;86(1):51-8.

Olabinri BM, Odedire OO, Olaleye MT, Adekunle AS, Ehigie LO, Olabinri PF. In vitro evaluation of hydroxyl and nitric oxide radical scavenging activities of artemether. Res J Biol Sci 2010;5(1):102-5.

Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agri Food Chem 1998;46:1887-92.

Halliwell B. Protection against tissue damage in vivo by desferrioxamine: What is its mechanism of action? Free Radic Biol Med 1989;7(6):645 51.

Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OI. On the in vitro antioxidative properties of melatonin. J Pineal Res 2002;33(3):167-71.

Zhao H, Fan W, Dong J, Lu J, Chen J, Shan L, et al. Evaluation of antioxidant activities and total phenolic contents of typical malting barley varieties. Food Chem 2008;107:296-304.

Wink DA, Kasprzak KS, Maragos CM, Elespuru RK, Misra M, Dunams TM, et al. DNA deaminating ability and genotoxicity of nitric oxide and its progenitors. Science 1991;254(5034):1001-3.

Houghton PJ. The role of plants in traditional medicine and current therapy. J Altern Complement Med 1995;1(2):131-43.

Sumathy R, Sankaranarayanan S, Bama P, Ramachandran J, Vijayalakshmi M, Deecaraman M. Antioxidant and antihemolytic activity of flavanoid extract from fruit peel of Punica granatum. Asian J Pharm Clin Res 2013;6(2):211-4.

Published

01-11-2014

How to Cite

G, B. ., and P. K. M. “A Study on Phytochemical Properties and Antioxidant Activity of Different Solvent Extract of Siddha Drug Karisalai Karpam Chooranam in Invitro Condition”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 5, Nov. 2014, pp. 250-4, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/2847.

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