CURCUMIN PROTECTION AGAINST CADMIUM-INDUCED OXIDATIVE STRESS IN PANCREAS OF SWISS ALBINO MICE

Suman Sharma; Anu Anu

doi:10.22159/ajpcr.2018.v11i11.28700

Authors

Suman Sharma Department of Zoology and Environmental Sciences, Punjabi University, Patiala - 147 002, Punjab, India.
Anu Anu Department of Zoology and Environmental Sciences, Punjabi University, Patiala - 147 002, Punjab, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.28700

Keywords:

Cadmium chloride, Curcumin, Oxidative stress, Glutathione peroxidase, Pancreas

Abstract

Objective: This study was planned to explore the protective efficacy of Curcuma longa (curcumin) against cadmium chloride (CdCl2)-induced oxidative stress in pancreatic tissue of albino mice.

Methods: A total of 40 albino mice were equally divided into eight groups (five mice for each). The mice were kept on standard feed and water ad libitum. Control Groups 1 and 5 mice were given equal amount of distilled water, Groups 2 and 6 mice received 1 mg/kg body weight of CdCl2 on alternate days, and Group 3 and 7 received 1 mg/kg body weight of CdCl2 on alternate days and 100 mg/kg body weight of curcumin every day. Groups 4 and 8 received 100 mg/kg body weight of curcumin daily and were kept as positive control. Autopsies were done on 15 and 45 days post-treatment. The pancreas was collected from each mouse and homogenized for biochemical study.

Results: The results of the present study revealed that Cd-induced oxidative stress depleted the antioxidant scavenger system and curcumin showed free radical scavenging potential. The activities of antioxidant enzymes, i.e., superoxide dismutase, catalase, glutathione peroxidase were observed to be significantly decreased, and methylenedioxyamphetamine concentration was increased in CdCl2-treated group which was restored to normal level after curcumin treatment.

Conclusion: It can be concluded that curcumin acts as a powerful and beneficial antioxidant against oxidative stress by scavenging the free radicals produced by CdCl2.

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References

Voldner EC, Alvo M. Estimation of wet deposition of sulfur, nitrogen, cadmium and lead to the Great Leaks. Environ Sci Technol 1993;27:292 8.

Bao RK, Zheng SF, Wang XY. Selenium protects against cadmium-induced kidney apoptosis in chickens by activating the PI3K/AKT/Bcl-2 signaling pathway. Environ Sci Pollut Res Int 2017;24:20342-53.

Sun B, Xing M. Evaluated the twenty-six elements in the pectoral muscle of as-treated chicken by inductively coupled plasma mass spectrometry. Biol Trace Elem Res 2016;169:359-64.

Martin MH, Nriagu JO. Cadmium in the environment. Part 2. Health effects. J Ecol 1998;71:343.

Li JT, Qiu JW, Wang XW, Zhong Y, Lan CY, Shu WS, et al. Cadmium contamination in orchard soils and fruit trees and its potential health risk in Guangzhou, China. Environ Pollut 2006;143:159-65.

Wang L, Zhou X, Yang D, Wang Z. Effects of lead and/or cadmium on the distribution patterns of some essential trace elements in immature female rats. Human Exp Toxicol 2011;30:1914-23.

Uetani M, Kobayashi E, Suwazono Y, Honda R, Nishijo M, Nakagawa H, et al. Tissue cadmium (Cd) concentrations of people living in a cd polluted area, Japan. Biometals 2006;19:521-5.

El-Muayed M, Raja MR, Zhang X, MacRenaris KW, Bhatt S, Chen X, et al. Accumulation of cadmium in insulin-producing beta cells. Islets 2012;4:405-16.

Patra RC, Swarup D, Senapati SK. Effects of cadmium on lipid peroxides and superoxide dismutase in hepatic, renal and testicular tissue of rats. Vet Hum Toxicol 1999;41:65-7.

Chopra M, Rani S, Sharma VL. Evaluating the protective efficacy of combination and Withania somnifera and vitamin E against cadmium induced oxidative stress mediated hepatic histopathology and genotoxicity in murine model. Int J Pharm Pharm Sci 2017;9:230-5.

Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction? Diabetes 2003;52:1-8.

Han JC, Park SY, Hah BG, Choi GH, Kim YK, Kwon TH, et al. Cadmium induces impaired glucose tolerance in rat by down-regulating GLUT4 expression in adipocytes. Arch Biochem Biophys 2003;413:213-20.

Suzuki KT, Ohnuki R, Yaguchi K, Yamada YK. Accumulation and chemical forms of cadmium and its effect on essential metals in rat spleen and pancreas. J Toxicol Environ Health 1983;11:727-37.

Liu J, Qu W, Kadiiska MB. Role of oxidative stress in cadmium toxicity and carcinogenesis. Toxicol Appl Pharmacol 2009;238:209-14.

El-Bahr SM. Biochemistry of free radicals and oxidative stress. BMC Complementary Altern Med 2013;1:111-7.

MatEs JM, Perez-Gmez C, De Castro IN. Antioxidant enzymes and human diseases. Clin Biochem 1999;32:595-603.

Lei LJ, Jin TY, Zhou YF. Insulin expression in rats exposed to cadmium. Biomed Environ Sci 2007;20:295-301.

Kunchandy E, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharmacol 1990;58:237-40.

Sharma OP. Antioxidant activity of curcumin and related compounds. Biochem Pharmacol 1976;25:1811-2.

Deng SL, Chen WF, Yang BZ, Liu ZL. Protective effects of curcumin and its analogues against free radical-induced oxidative haemolysis of human red blood cells. Food Chem 2006;98:112-19.

Sharma S, Anu KA. Effect of Curcuma longa supplementation on lipid peroxidation, serum amylase, lipase activites in mice exposed to cadmium. Int J Adv Res 2017;5:786-92.

Huang MT, Lou YR, Ma W, Newmark HL, Reuhl KR, Conney AH, et al. Inhibitory effects of dietary curcumin on forestomach, duodenal, and colon carcinogenesis in mice. Cancer Res 1994;54:5841-7.

Rao CV, Rivenson A, Simi B, Reddy BS. Chemoprevention of colon carcinogenesis by dietary curcumin, a naturally occurring plant phenolic compound. Cancer Res 1995;55:259-66.

Baliga MS, Katiyar SK. Chemoprevention of photocarcinogenesis by selected dietary botanicals. Photochem Photobiol Sci 2006;5:243-53.

Panday U, Chandra A, Awasthi S, Jin GF, Piper JT, Godley BF, et al. Attenuation of galactose cataract by low levels of dietary curcumin. Nutr Res 2000;20:515-26.

Suryanarayana P, Krishnaswamy K, Reddy GB. Effect of curcumin on galactose-induced cataractogenesis in rats. Mol Vis 2003;9:223-30.

Suryanarayana P, Saraswat M, Mrudula T, Krishna TP, Krishnaswamy K, Reddy GB, et al. Curcumin and turmeric delay streptozotocin-induced diabetic cataract in rats. Invest Ophthalmol Vis Sci 2005;46:2092-9.

Mukhopadhyay A, Basu N, Ghatak N, Gujral PK. Anti-inflammatory and irritant activities of curcumin analogues in rats. Agents Actions 1982;12:508-15.

Kuroda M, Mimaki Y, Nishiyama T, Mae T, Kishida H, Tsukagawa M, et al. Hypoglycemic effects of turmeric (Curcuma longa L. Rhizomes) on genetically diabetic KK-ay mice. Biol Pharm Bull 2005;28:937-9.

Sajithlal GB, Chithra P, Chandrakasan G. Effect of curcumin on the advanced glycation and cross-linking of collagen in diabetic rats. Biochem Pharmacol 1998;56:1607-14.

Jain SK, Rains J, Jones K. Effect of curcumin on protein glycosylation, lipid peroxidation, and oxygen radical generation in human red blood cells exposed to high glucose levels. Free Radic Biol Med 2006;41:92 6.

Nanda A, Gupta N. Efficacy of turmeric on blood glucose and polyol pathway in diabetic albino rats. Plant Foods Hum Nutr 2002;57:41-52.

Fujisawa S, Atsumi T, Ishihara M, Kadoma Y. Cytotoxicity reactive oxygen species-generating activity and radical scavenging activity of curcumin and other related compounds. Anti-Cancer Res 2004;24:563 70.

Spinas GA. The dual role of nitric oxide in islets Î²-cells. News Physiol Sci 1999;14:49-54.

Pan MH, MLin-Shiiau SY, Lin JK. Comparative studies on the suppression of nitric oxide synthase by curcumin and its hydrogenated metabolites through down-regulation of ikappa bkinase and NFkappaB activation in macrophages. Biochem Pharmacol 2000;60:1665-76.

Kalpana C, Menon VP. Curcumin ameliorates oxidative stress during nicotine-induced lung toxicity in wistar rats. Ital J Biochem 2004;53:82 6.

Polasa K, Naidu AN, Ravindranath I, Krishnaswamy K. Inhibition of B(a)P induced strand breaks in presence of curcumin. Mutat Res 2004;557:203-13.

Kamel R, Hashim AA, Ali SA. Palliative effect of curcumin on stz-induced diabetes in rats. Int J Pharm Pharm Sc 2014;6:558-63.

Wilbur KM, Bernheim F, Shapiro OW. The thiobarbituric acid reagent as a test for oxidation of unsaturated fatty acids by various agents. J Biochem Biophys 1949;24:305-13.

Das K, Samamta L, Chainy GB. A modified spectrophotometric assay for superoxide dismutase using nitrite formation by superoxide radicals. Indian J Biochem Biophys 2009;2:43-50.

Aebi HE. Catalase. In: Bergmeyr HU, editor. Methods of Enzymatic Analysis. Vol. 3. Weinheim: Verlag Chemie; 1983. p. 273-86.

Ahrens RA. Glutathione peroxidase: A role for selenium (Rotruck 1972). J Nutr 1997;127:1052S-1053S.

Lenzen S, Drinkgern J, Tiedge M. Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues. Free Radic Biol Med 1996;20:463-6.

Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 2011;2011:457327.

Sargazi M, Zal F, Karbalaei N. Effects of cadmium on oxidative stress markers, and insulin secretion and content in pancreatic isolated islet of rats. J. Mazandaran Univ Med Sci 2018;27:1-14.

Liu R, Jia T, Cui Y, Lin H, Li S. The protective effect of selenium on the chicken pancreas against cadmium toxicity via alleviating oxidative stress and autophagy. Biol Trace Elem Res 2017;184:240-6.

Palati DJ, Vanapatla SR. Protective role of Aerva monsoniae and selenium on cadmium-induced oxidative liver damage in rats. Asian J Pharm Clin Res 2018;11:177-81.

Waisberg M, Joseph P, Hale B, Beyersmann D. Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology 2003;192:95-117.

Bashir N, Manoharan V, Miltonprabu S. Grape seed proanthocyanidins protects against cadmium induced oxidative pancreatitis in rats by attenuating oxidative stress, inflammation and apoptosis via nrf-2/ HO-1 signaling. J Nutr Biochem 2016;32:128-41.

Lyons TJ. Oxidized low-density lipoproteins, a role in the pathogenesis of atherosclerosis in diabetes. Diabet Med 1991;8:411-9.

Suryanarayana P, Satyanarayana A, Balakrishna N, Kumar PU, Reddy GB. Effect of turmeric and curcumin on oxidative stress and antioxidant enzymes in streptozotocin-induced diabetic rat. Med Sci Monit 2007;13:BR286-92.

Abdel Aziz MT, El-Asmar MF, El-Ibrashy IN, Rezq AM, Al-Malki AL, Wassef MA, et al. Effect of novel water soluble curcumin derivative on experimental Type- 1 diabetes mellitus (short term study). Diabetol Metab Syndr 2012;4:30.

Halliwell B. Lipid peroxidation, antioxidants and cardiovascular disease: How should we move forward? Cardiovasc Res 2000;47:410 8.

Sarkar S, Yadav P, Bhatnagar D. Lipid peroxidative damage on cadmium exposure and alterations in antioxidant system in rat erythrocytes: A study with relation to time. Biometals 1998;11:153-7.

Jacob RA, Burri RA. Oxidative damage and defence. Am J Clin Nutr 1996;63:985-90.

Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995;41:1819-28.

Sevcikova M, Modra H, Slaninova A, Svobodova Z. Metals as a cause of oxidative stress in fish: A review. Vet Med Czech 2011;56:537-46.

Yao XH, Chen L, Nyomba BL. Adult rats prenatally exposed to ethanol have increased gluconeogenesis and impaired insulin response of gluconeogenic. Genes J Appl Physiol 2006;100:642-8.

Casalino E, Calzaretti G, Sblano C, Landriscina C. Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium. Toxicology 2002;179:37-50.

Shukla G, Chiu J, Hart BA. Cd induced elevation in the gene expressions of the regulatory subunit of glutamylcysteine synthetase in rat lung and alveolar epithelial cells. Toxicology 2000;151:45-54.

Masuda T, Maekawa T, Hidaka K, Bando H, Takeda Y, Yamaguchi H. Chemical studies on antioxidant mechanism of curcumin: Analysis of oxidative coupling products from curcumin and linoleate. J Agric Food Chem 2001;49:2539-47.

Dinkova-Kostova AT, Talalay P. Direct and indirect antioxidant properties of inducers of cytoprotective proteins. Molecular Nutr Food Res 2008;52:128-38.

Chaudhary B, Agarwal S, Bist R. Curcumin amends oxidative stress and antioxidants status in olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla of mice acutely intoxicated with lindane. Int J Pharm Pharm Sci 2016;8:244-8.

Adhikari R, Jyothi Y, Bora D, Veena V. A combined effect of aqueous extract of Curcuma longa linn. with metformin in diabetes induced neuroptahic pain in rats. Asian J Pharm Clin Res 2015;8:166-70.

El-Bahr SM, El-Sabagh IM. Hepatic gene expression of insulin like growth factor and selected antioxidants in diabetic rats treated with turmeric or black cumin seed. Br Biotechnol J 2014;4:778-93.

Arun N, Nalini N. Efficacy of turmeric on blood glucose and polyol pathway in diabetic albino rats. Plant Foods Hum Nutr 2002;57:41-52.

Sharma S, Kulkarni SK, Chopra K. Curcumin, the active principle of turmeric (Curcuma longa), ameliorates diabetic nephropathy I rats. Clin Exp Pharmacol Physiol 2002;33:940-5.

Hussein HK, Abu-Zinadah OA. Antioxidant effect of curcumin extracts in induced diabetic wister rats. Int J Zool Res 2010;6:266-76.

Venkatesan N, Punithavathi D, Arumugan V. Curcumin prevents adriamycin nephrotoxicity in rats. Br J Pharmacol 2000;12:231-234.

Biswas SK, McClure D, Jimenez LA, Megson IL, Rahman I. Curcumin induces glutathione biosynthesis and inhibits NFkappaB activation and interleukin-8 release in alveolar epithelial cells: mechanism of free radical scavenging activity. Antioxid Redox Signal 2005;7:32-41.

Reddy AC, Lokesh BR. Studies on the jnhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous ion. Mol Cell Biochem 1994;137:1-8.

Attia AM, Ibrahim FA, El-Latif NA, Aziz SW. Antioxidant effects of curcumin against cadmium chloride-induced oxidative stress in the blood of rats. J Pharmacog Phytother 2014;6:33-40.

Khattab HA, Al-Faleh AA, Al-Amoudi NS. Effect of ginger, curcumin and their mixture on blood glucose and lipids in diabetic rats. Life Sci J 2013;10:428-42.

Hussein MA, EL-Maksoud HA. Biochemical effects of resveratrol and curcumin combination on obese diabetic rats. J Mol Clin Pharm 2013;4:1-10.

Seo KI, Myung-Sook C, Ju JU, Kim HJ, Jiyoung Y, Jeon SM, et al. Effect of curcumin supplementation on blood glucose, plasma insulin, and glucose homeostasis related enzyme activities in diabetic db/db mice. Mol Nutr Food Res 2008;52:995-1004.