PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF PLECTRANTHUS BOURNEAE GAMBLE (LAMIACEAE)

Authors

  • R Thaniarasu Bharathidasan University
  • T Senthil Kumar Bharathidasan University
  • M. N Abubacker National College, Bharathidasan University
  • M. V Rao Bharathidasan University

Abstract

Objective: The aim was to investigate the antibacterial activity of Plectranthus bourneae Gamble (Lamiaceae) using different solvents.
Methods: Petroleum ether, chloroform, acetone, ethanol and aqueous leaves extracts at different concentration (25, 50, 75, 100 μg/ml) were
evaluated for antibacterial activity using disc diffusion method against some Gram-positive species namely Bacillus subtilis, Clostridium sporogenes,
Staphylococcus aureus, Streptococcus mutans, Streptococcus mitis and Gram-negative species Salmonella typhi, Klebsilla pneumoniae, Entrobactor
aerogenes, Citrobactor freundii, Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bacterial concentrations (MBC) were
determined by serial dilution method.
Results: The antibacterial activity results of ethanol extract (50 μg/ml) showed maximum zone of inhibition (26 mm) against S. aureus and MIC
value of 0.78 μg/ml and MBC of 1.56 μg/ml whereas aqueous extract was least effective on all the strains. The Gram-positive strains of S. aureus and
S. mutans were found to be the most sensitive and resistance on all the extracts. Except S. typhi, Gram-negative bacteria used were less inhibitory
effect to all the extracts.
Conclusion: The present study concluded that the ethanolic extracts of P. bourneae possess potential antibacterial activities against certain
microorganisms.

Keywords: Plectranthus bourneae, Antibacterial activity, Disc diffusion method, Lamiaceae.

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Author Biographies

R Thaniarasu, Bharathidasan University

Department of Plant Science, Doctoral Research Student.

T Senthil Kumar, Bharathidasan University

Assistant Professor, Department of Industry University collabration.

M. N Abubacker, National College, Bharathidasan University

Associate professor and Head, Department of Biotechnology.

M. V Rao, Bharathidasan University

Honarary Professor, Department of Plant Science

References

Lukhoba C W, Simmonds MS, Paton AJ. Plectranthus: a review of

ethnobotanical uses. J Ethnopharmacol 2006;103(1):1-24.

Abdel-Mogib M, Albar HA, Batterjee SM. Chemistry of the genus

Plectranthus. Molecules 2002;7:271-301.

Morton JF. Country borage (Coleus aromaticus Lour.): A potent flavoring

and medicinal plant. J Herbs Spices Med Plants 1992;1(77):1- 2.

Abulfaith HA. Medicinal Plants in Southwestern Saudi Arabia.

Khamis: Al Thaghr Press: 1987. p. 162.

Gurgel AP, da Silva JG, Grangeiro AR, Oliveira DC, Lima CM, da

Silva AC, et al. In vivo study of the anti-inflammatory and antitumor

activities of leaves from Plectranthus amboinicus (Lour.) Spreng

(Lamiaceae). J Ethnopharmacol 2009;125:361-3.

Matu EN, van Staden J. Antibacterial and anti-inflammatory activities

of some plants used for medicinal purposes in Kenya. J Ethnopharmacol

;87(1):35-41.

Gaspar-Marques C, Simões MF, Duarte A, Rodríguez B. Labdane

and kaurane diterpenoids from Plectranthus fruticosus. J Nat Prod

;66(4):491-6.

Wellsow J, Grayer RJ, Veitch NC, Kokubun T, Lelli R, Kite GC,

et al. Insect-antifeedant and antibacterial activity of diterpenoids from

species of Plectranthus. Phytochemistry 2006;67(16):1818-25.

Runyoro DK, Matee MI, Ngassapa OD, Joseph CC, Mbwambo ZH.

Screening of Tanzanian medicinal plants for anti-Candida activity.

BMC Complement Altern Med 2006;6:11.

Murthy PS, Ramalakshmi K, Srinivas P. Fungitoxic activity of

Indian borage (Plectranthus ambonicus) volattiles, Food Chem

;114:1014-8.

Matthew KM. Precursory notes on the flora of the Palni (Pulney) Hills,

South India: II. Kew Bull 1993;48:757-65.

Matthew KM. Flora of Palni Hills. Suppl. III. Tiruchirapalli: Rapinat

Herbarium; 1998. p. 1117.

Harborne JB. Phytochemical Methods. A Guide to Modern Techniques

of Plant Analysis. London: Chapman and Hall; 1984.

Khandelwal KR. Practical Pharmacognosy Techniques and

Experiments. 17th ed. Pune: Nirali Prakashan; 2007.

Stokes EJ. Clinical Bacteriology. London: Edward Arnold Ltd.; 1975.

p. 208.

Sandberg F, Bruhn JG. Screening of plants for biologically active

substances. In: Soforowa EA, editor. African Medicinal Plants. Lagos:

University of Ife Press; 1979. p. 119.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol

Rev 1999;12(4):564-82.

Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borisjuk

N, et al. Plants and human health in the twenty-first century. Trends

Biotechnol 2002;20(12):522-31.

Egwaikhide PA, Gimba CE. Analysis of the phytochemical and

anti- microbial actvity of Plectranthus glandulosis whole plant. Middle

East J Sci Res 2003;2(3-4):135-8.

Okwu DE. Evaluation of the chemical composition of indigenous

spices and flavouring agent. Glob J Pure Appl Sci 2001;7(3):455-9.

Sofowora A. Medicinal Plants and Traditional Medicine in Africa.

New York: John Wiley and Son Ltd.; 1993. p. 150-3.

Rijo P, Rodriguez B, Duarte A, Simoes MF. Antimicrobial properties of

Plectranthus ornatus extracts, 11-acetoxyhalima-5, 13-dien-15-oic acid

metabolite and its derivatives. J Nat Prod 2011;1:57-64.

Oyedemi SO, Afolayan AJ. Antibacterial and antioxidant activities of

hydroalcoholic stem bark extract of Schotia latifolia Jacq. Asian Pac J

Trop Med 2011;4(12):952-8.

Ullah MO, Haque M, Urmi KF, Zulfiker AH, Anita ES, Begum M, et al.

Anti-bacterial activity and brine shrimp lethality bioassay of methanolic

extracts of fourteen different edible vegetables from Bangladesh. Asian

Pac J Trop Biomed 2013;3(1):1-7.

Bhattacharya S, Zaman MK, Haldar PK. Antibacterial activity of stem

bark and root of Indian Zanthoxylum nitidum. Asian J Pharm Clin Res

;2(1):30-4.

Venkatesh Krishna V, Girish Kumar K, Pradeepa K, Santhosh

Kumar SR. Antibacterial activity of ethanol extract of Musa paradisiaca

CV. Puttable and Musa acuminate CV. Grand naine. Asian J Pharm Clin

Res 2013;6(2):169-72.

Published

01-01-2015

How to Cite

Thaniarasu, R., T. Senthil Kumar, M. N. Abubacker, and M. V. Rao. “PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF DIFFERENT SOLVENT EXTRACTS OF PLECTRANTHUS BOURNEAE GAMBLE (LAMIACEAE)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 1, Jan. 2015, pp. 79-82, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/2899.

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