ISOLATION AND CHARACTERIZATION OF Î²-SITOSTEROL FROM ELAEAGNUS ANGUSTIFOLIA CULTIVATED IN IRAQ

RAND A  AZEEZ; Ibrahim S Abaas; Enas J Kadhim

doi:10.22159/ajpcr.2018.v11i11.29030

Authors

RAND A AZEEZ Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Al Yarmouk University College, Baghdad, Iraq.
Ibrahim S Abaas Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Al Yarmouk University College, Baghdad, Iraq.
Enas J Kadhim Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Al Yarmouk University College, Baghdad, Iraq.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.29030

Keywords:

Elaeagnus angustifolia, Nil, 1H nuclear magnetic resonance

Abstract

Objective: The aim of our study was to investigate chemical constituents of leaves and seeds of Elaeagnus angustifolia since no phytochemical investigation had been done previously in Iraq.

Methods: Phytochemical screening of the extracts obtained from the leaves of E. angustifolia indicated the presence of flavonoids (type of flavonols), saponins, alkaloids, steroids, and terpenoids. Thin-layer chromatography fingerprinting and the spraying reagent (concentrated H2SO4 and vanillin in ethanol) were used to identify the hexane extract containing phytosterols.

Results: The different chromatographic and spectroscopic results revealed the presence of Î²â€sitosterol isolated from E. angustifolia.

Conclusion: The isolation and purification afforded white crystalline powder which was subjected to chemical and spectral identification by infrared and 1H-nuclear magnetic resonance. The compound was identified as Î²â€sitosterol.

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