ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY

GOMATHI KANNAYIRAM; SANDHYA A; SOWMIYA S; VALARMATHI S; DANIEL JOSEPH

doi:10.22159/ajpcr.2019.v12i2.29775

Authors

GOMATHI KANNAYIRAM Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India. http://orcid.org/0000-0003-0814-3383
SANDHYA A Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.
SOWMIYA S Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.
VALARMATHI S Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.
DANIEL JOSEPH Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i2.29775

Keywords:

Nigella sativa, Fourier transform infrared, Nanoparticles, Protein denaturation, Enzyme-linked immunosorbent assay

Abstract

Objective: The aim of this study is to evaluate the anti-inflammatory activity of Nigella sativa silver nanoparticles (NS AgNPs).

Methods: Fourier transform infrared analysis was used to characterize the NS AgNPs and the extract. 2,2-diphenylpicrylhydrazyl assay was done to test the antioxidant potency of NS AgNP. Furthermore, in vitro anti-inflammatory activity of the extract and the NS AgNP was determined by red blood cell (RBC) membrane stabilization assay, protein inhibition assay, and interleukin-1 (IL-1) beta assay.

Results: The NS AgNP exhibited dose-dependent antioxidant property. At the concentration 0.01 mg/ml 80% of radical was scavenged by NS AgNP. Inhibition of protein denaturation assay also suggests that NS AgNP shows the highest activity (70%) when compared with the standard drug aspirin (65%). RBC assay suggests that NS AgNP stabilizes the RBC membrane and prevents leaking. In the enzyme-linked immunosorbent assay method the NS AgNP showed better IL-1 beta inhibition activity when compared to aqueous extract.

Conclusion: From the study, it was inferred that NS AgNPs are more effective when compared to the extract. These results suggest that NS AgNP can be used to treat inflammatory disorders.

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Author Biography

GOMATHI KANNAYIRAM, Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.

DEPARTMENT OF BIOTECHNOLOGY DR. M.G.R.EDUCATIONAL AND RESEARCH INSTITUTE

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