ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i4.30500Keywords:
Knema attenuata, folk use, anti inflammatory activity, carrageenan induced paw oedemaAbstract
Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models.
Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells.
Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes.
Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.
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Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol 2007;147:227-35.
Feghali CA, Wright TM. Cytokines in acute and chronic inflammation. Front Biosci 1997;2:d12-26.
Walter JB, Talbot IC. General Pathology. 7th ed. London: Churchill Livingstone; 2009.
Khanna HD, Karki K, Pande D, Negi R, Khanna RS. Inflammation, free radical damage, oxidative stress and cancer. Int J Inflamm Cancer Integr Ther 2014;1:109.
Gui T, Shimokado A, Sun Y, Akasaka T, Muragaki Y. Diverse roles of macrophages in atherosclerosis: From inflammatory biology to biomarker discovery. Mediators Inflamm 2012;2012:693083.
Osadebe PO, Okoye FB. Anti-inflammatory effects of crude methanolic extract and fractions of Alchornea cordifolia leaves. J Ethnopharmacol 2003;89:19-24.
McGettigan P, Henry D. Use of non-steroidal anti-inflammatory drugs that elevate cardiovascular risk: An examination of sales and essential medicines lists in low middle and high-income countries. PLoS Med 2013;10:e1001388.
Krishnaveni J, Mangai AS, Jency MI. In vitro anti-inflammatory activity of blue-green algae Geitlerinema splendidum collected from Western Ghats, South India. Asian J Pharm Clin Res 2018;11:407-9.
Available from: http://www.indiabiodiversityorg/biodiv/species/show/13965.
Ravikumar K, Ved DK. One Hundred Red Listed Medicinal Plants of Conservation Concern in Southern India. Bangalore: Foundation for Revitalisation of Local Health Traditions; 2000. p. 219-22.
Joshi BS, Ravindranath KR, Viswanathan N. Structure and stereochemistry of attenuol, a new lignan from Knema attenuata (Wall.) Warb. Experientia 1978;34:422.
Korkina LG. Phenylpropanoids as naturally occurring antioxidants: From plant defense to human health. Cell Mol Biol (Noisy-le-grand) 2007;53:15-25.
Pinto MM, Kijjoa A. Chemical study of Knema species from Thailand. Quim Nova 1990;13:243-4.
Khadabadi SS, Deore BS. Pharmacognosy and Phytochemistry: Experimental Phytopharmacognosy a Comprehensive Guide. 2nd ed. Mumbai: Nirali Prakasan; 2014.
Joo T, Sowndhararajan K, Hong S, Lee J, Park SY, Kim S, et al. Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L. Saudi J Biol Sci 2014;21:427-35.
Freshney RI. Culture of Animal Cells: A Manual of Basic Techniques. 5th ed. John Wiley and Sons, Inc.; 2005. p. 321.
Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind Paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med 1962;111:544-7.
Winter CA, Porter CC. Effect of alterations in side chain upon anti-inflammatory and liver glycogen activities of hydrocortisone esters. J Am Pharm Assoc Am Pharm Assoc 1957;46:515-9.
Kurian NK, Nair HP, Bhat SG. Evaluation of anti-inflammatory property of melanin from marine Bacillus Spp. BTCZ31. Asian J Pharm Clin Res 2015;8:251-5.
Walker MC, Gierse JK. In vitro assays for cyclooxygenase activity and inhibitor characterization. Methods Mol Biol 2010;644:131-44.
Axelrod B, Cheesebrough TM, Laakso S. Lipoxygenase from soyabean. Methods Enzymol 1981;71:441-5.
Lepoivre M, Chenais B, Yapo A, Lemaire G, Thelander L, Tenu JP, et al. Alterations of ribonucleotide reductase activity following induction of the nitrite-generating pathway in adenocarcinoma cells. J Biol Chem 1990;265:14143-9.
Necas J, Bartosikova L. Carrageenan: A review. Vet Med 2013;58:187-205.
Hendra P, Fenty, Andreani PR, Pangestuti B, Julianus J. Evaluation of antihyperlipidemic, anti-inflammatoryand analgesic activities of Eurycoma longifolia in animal models. Int J Pharm Pharm Sci 2017;9:166-9.
Chaithanya KK, Gopalakrishnan VK, Hagos Z, Rao GD. Mesuaferrin A-bioactive flavanoid isolated from the bark of Mesua ferrea L., against phospholipase A2, Cyclooxygenase and lipooxygenase: An in vitro, in vivo and in silico approach. Int J Pharm Pharm Sci 2017;10:102-6.
Swingle KF, Shideman FE. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacol Exp Ther 1972;183:226-34.
Rogero S. Lugao A. Ichikawa IT, Cruz AS. In vitro cytotoxicity test: A comparative study between two methodologies. Mater Res 2003;3:317-20.
Neda MD Simin N, Svirčev E, Orčić D. Lipid peroxidation. The Effect of Plant Secondary Metabolites on Lipid Peroxidation and Eicosonoid Pathway. Ch. 9. USA: Intech.; 2012. p. 204-6.
Vinayachandra, Chandrashekhar KR. Phenolic contents of Knema attenuata fruits and their bioactive potentials. J Herbs Spices Med Plants 2014;20:183-95.
Ashok P, Koti BC, Thippeswamy AH, Tikare VP, Dabadi P, Viswanathaswamy AH, et al. Evaluation of antiinflammatory activity of Centratherum anthelminticum (L) kuntze seed. Indian J Pharm Sci 2010;72:697-703.
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