ANTIBACTERIAL ACTIVITY OF AMORPHOPHALLUS KONKANENSIS AND AMORPHOPHALLUS BULBIFER TUBER
Abstract
Objective: Evaluation of antibacterial activity of unexplored, endemic Amorphophallus konkanensis (AKT) Hett., Yadav & Patil and Amorphophallus
bulbifer (ABT) (Roxb.) Bl.
Methods: Acetone and ethanol extracts of tubers of AKT and ABT were evaluated for in vitro antibacterial activity against Bacillus subtilis, Micrococcus
aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae using agar diffusion method, minimum inhibitory
concentration (MIC), minimum bactericidal concentration (MBC) and growth pattern of test organisms. High-performance liquid chromatography
(HPLC) analysis was carried out for active phenolic compounds.
Results: AKT and ABT extracts for both solvents showed significant antibacterial activities. The zone of inhibition was ranged from 12-25 mm to 11-
23 mm for AKT and ABT extracts respectively. Highest significant activity was observed against M. aureus. The MIC values of acetone extracts were
ranged from 200-350 μg/ml (AKT) to, 200-400 μg/ml (ABT). Ethanol extracts of AKT and ABT were showed MIC at 250-450 μg/ml and 300-450 μg/
ml respectively. MBC value of acetone extracts ranged from 260-460 μg/ml (AKT) to 300-500 μg/ml (ABT) and for ethanol extracts 340-500 μg/ml
(AKT) and 380-540 μg/ml (ABT). The effect of extracts on growth of microorganisms was also studied. HPLC analysis showed phenolic compounds
viz. tannic acid, gallic acid, quercetin, p-coumaric acid, and catechin. This polyphenolic rich extracts of AKT and ABT have demonstrated various
degree of microbial growth inhibition.
Conclusion: Antibacterial activity of AKT and ABT implies that, these plants have the potential for preparation of alternative medicines for infections
caused by microorganisms that have become resistant to the current therapeutic measures.
Keywords: Amorphophallus konkanensis, Amorphophallus bulbifer, Antibacterial activity, Minimum inhibitory concentration, minimum bactericidal
concentration, High-performance liquid chromatography.
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