The TO STUDY ANTIBIOTIC SUSCEPTIBILITY PATTERN OF STAPHYLOCOCCUS AUREUS ISOLATED FROM VARIOUS SKIN SAMPLES

NEHA; BHARTI MITTU; ZARINA BEGUM; HARJOT PAL KAUR

doi:10.22159/ajpcr.2019.v12i7.32868

Authors

NEHA Department of Life Science (Bio-Technology), I. K. Gujral Punjab Technical University, Jalandhar, Punjab, India.
BHARTI MITTU Department of Life Science (Bio-Technology), I. K. Gujral Punjab Technical University, Jalandhar, Punjab, India.
ZARINA BEGUM Department of Life Science (Bio-Technology), I. K. Gujral Punjab Technical University, Jalandhar, Punjab, India.
HARJOT PAL KAUR Department of Life Science (Bio-Technology), I. K. Gujral Punjab Technical University, Jalandhar, Punjab, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i7.32868

Keywords:

Staphylococcus aureus,, Antibiotic,, Zone of inhibition,, Minimum inhibitory concentration

Abstract

Objective: The study was carried out to check the antibiotic susceptibility profile of Staphylococcus aureus.

Methods: In this study for the isolation of strain, skin swabs from different parts of the body were taken aseptically with the set protocol. The sensitivity patterns of S. aureus were explored with different antibiotics for the treatment of S. aureus infections. We have studied the susceptibility pattern of various antibiotics including present all line therapies and other antibiotics using disk-diffusion method.

Results: It has been found that bacteria show the highest sensitivity toward third-line antibiotic tetracycline, trimethoprim, and minocycline. The different concentrations of these antibiotics were used to check the minimum inhibitory concentration and found to be effective even at the lower concentration of 0.01 mcg/disc. Furthermore, the minimum inhibitory concentration of each antibiotic has been calculated to find the most effective antibiotic at low concentration. The isolates are found to be highly resistant toward ampicillin, ciprofloxacin, and cefotaxime.

Conclusion: The research concluded that the bacteria S. aureus are found to be more sensitive for chloramphenicol, kanamycin, and trimethoprim in first-line antibiotics, second-line antibiotics, and third-line antibiotics, respectively.

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References

Samson OO, Anthony OE. Prevalence of multi-drug resistant Staphylococcus aureus in clinical specimens obtained from patients attending the university of Benin teaching hospital, Benin City, Nigeria. J Natural Sci 2013;3 Suppl 5:154-9.

Rahimi F, Bouzari M, Katouli M, Pourshafie MR. Antibiotic resistance pattern of methicillin resistant and methicillin sensitive Staphylococcus aureus in Tehran. Iran. Jundishapur J Microbiol 2013;6 Suppl 2:144-9.

Reynolds FJ. Biology 2420 Lab Manual. Richland College, New York: Fall Publishers; 2011.

Onile BA, Odugbemi TO, Nwofor C. Antibiotic susceptibility of bacterial agents of Septicemia in Ilorin. Niger Med Pract 1985;9 Suppl 4:16-8.

Meyers HF, Ernest J, Allan G. A review of Medical Pharmacology. Vol. 49. Los Altos: Lange Medical Publishers; 1970. p. 467-74.

Oskay M, Oskay D, Kalyoneu F. Activity of some plant extracts against multidrug resistant human pathogens. Iran J Pharm Res 2009;8 Suppl 4:293-300.

McGowan JE. Resistance in non-fermenting gram-negative bacteria: Multidrug resistance to the maximum. Am J Infect Control 2006;34:29 37.

Thornsberry C, McDougal LK. Successful use of broth microdilutio in susceptibility tests for methicillin-resistant (heteroresistant) staphylococci. J Clin Microbiol 1983;18:1084-91.

Bergey DH, Krieg NR, Holt JG. Bergey’s Manual of Systematic Bacteriology. Baltimore, MD: Williams and Wilkins; 1984.

Akpaka PE, Monecke S, Swanston WH, Rao AC, Schulz R, Levett PN. Methicillin sensitive Staphylococcus aureus producing panton-valentine leukocidin toxin in Trinidad and Tobago: A case report. J Med Case Rep 2011;5:157.

Kaur B, Balgir P, Mittu B, Chauhan A. Purification and physicochemical characterization of anti Gardnerella vaginalis bacteriocin HV6b produced by Lactobacillus fermentum isolate from human vaginal ecosystem bacteriocin HV6b produced by Lactobacillus fermentum isolate from human vaginal ecosystem. Am J Biochem Mol Biol 2013;3 Suppl 1:91-100.

Oyeleke SB, Manga BS. Essentials of Laboratory Practicals in Microbiology. 1st ed. Minna, Nigeria: Tobest Publishers; 2008. p. 63-5.

Omoigberale MN, Iyamu MI, Amengialue OO, Egharevba AP, Edobor O. Antibiotic resistance pattern of Staphylococcus aureus isolated from high vaginal swab and urethral swab specimens. Am J Res Commun 2014;2:96-107.

Salau OA, Yakubu SE, Ado SA. Prevalence and antibiotic susceptibility pattern of Staphylococcus aureus from patients attending some selected hospitals in Samaru, Zaria, Nigeria. Int J Health Sci Res 2015;5:79-84.

Bhooshan S, Prasad J, Dutta A, Vandana KE, Mukhopadhyay C. Reduced susceptibility of MRSA to vancomycin. Int J Pharm Pharm Sci 2016;8:321-2.

Basavaraj CM, Peerapur B, Jyothi P. Drug resistance patterns of clinical isolates of Staphylococcus aureus in tertiary care center of South India. Int J Pharm Pharm Sci 2015;7:70-2.

Oncel T, Ica T, Akpan M. Beta-lactamase production rate and antimicrobial susceptibility of Staphylococcus aureus isolated from clinical and sub-clinical mastitis case in Turkey. J Vet Med 2004;155 Suppl 7:385-8.

Aligholi M, Emaneini M, Jabalameli F, Shahsavan S, Dabiri H, Sedaght H, et al. Emergence of high-level vancomycin-resistant Staphylococcus aureus in the imam Khomeini hospital in Tehran. Med Princ Pract 2008;17:432-4.

Olasupo NA, Fitzgerald DJ, Gasson MJ, Narbad A. Activity of natural antimicrobial compounds against Escherichia coli and Salmonella enterica serovar typhimurium. Lett Appl Microbiol 2003;37:448-51.

Madhusudhan NS, Deepa S, Shoba DN. Correlation of cefoxitin disc diffusion test and oxacillin disc diffusion test for detecting mecA mediated oxacillin resistant Staphylococcus aureus. J Pharm Biomed Sci 2011;10 Suppl 2:2230-7885.

Anderson ME, Lefebvre SL, Weese JS. Evaluation of prevalence and risk factors for methicillin-resistant Staphylococcus aureus colonization in veterinary personnel attending an international equine veterinary conference. Vet Microbiol 2008;129:410-7.

Kaur H, Dhir K, Kaur J, Mittu B, Chauhan A. Synthesis and evaluation of diorganotin (1V) and triorganotin (1V) derivatives of aspirin, paracetamol and metronidazole as antimicrobial agents. Am J Drug Discov Dev 2013;3 Suppl 1:13-22.

Lentino JR, Narita M, Yu VL. New antimicrobial agents as therapy for resistant gram-positive cocci. Eur J Clin Microbiol Infect Dis 2008;27:3-15.