COMPARISON OF THE EFFECTS OF NIGELLA SATIVA OIL AND LASER ON TREATMENTS ON EXPERIMENTAL WOUND HEALING IN RATS

RABAB HAJWAL AL-ZAMILY; SHROQ MOHAMED ABAS AL-TEMIMI

doi:10.22159/ajpcr.2019.v12i7.33407

Authors

RABAB HAJWAL AL-ZAMILY Department of physiology and medical Physics, of Medicine, Al-Qadisiyah University, Al Diwaniyah, Iraq. Email:
SHROQ MOHAMED ABAS AL-TEMIMI Department of physiology and medical Physics, of Medicine, Al-Qadisiyah University, Al Diwaniyah, Iraq.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i7.33407

Keywords:

NIGELLA sativa oil, wound healing

Abstract

Objective:The study investigated the influences of treatment with Nigella sativa oil and laser therapy on wound healing in rats. The period of the inflammatory phase reduced through both laser and N. sativa oil actions; though, the laser was more effective than N. sativa oil, with more significant results.

Methods: We used 52 healthy rats male and divided the rats randomly into three groups. The treatment protocols used included N. sativa oil in the first group, laser in the second group, and control in the third group. In all groups, we performed histopathologic evaluations on the 1st, 3rd, 5th, and 7th days after surgery.

Results: The proliferation phase of each treatment indicated an increase in the number of fibroblasts, as well as stimulation of collagen formation and synthesis.Positive results on the inflammatory, proliferation, and maturation phases for both treatments of wound healing.

Conclusion:Laser treatment was more effective than N. sativa oil in the first two phases of wound healing.

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References

Salman H, Kashmoola MA, Al-Waiz MM, Al-Sandooq TA. Differences between low level laser therapy and triamcinolone acetonide kenalog on healing of recurrent aphthous ulceration. Ann Coll Med Mosul 2008;34:35-41.

Santuzzi CH, Buss HF, Pedrosa DF, Freire MO, Nogueira BV, Gonçalves WL. Combined use of low level laser therapy and cyclooxygenase-2 selective inhibition on skin incisional wound reepithelialization in mice: A preclinical study. An Bras Dermatol 2011;86:278-83.

Adzick NS, Lorenz HP. Cells, matrix, growth factors, and the surgeon. The biology of scarless fetal wound repair. Ann Surg 1994;220:10-8.

Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003;83:835-70.

Schmidt C, Fronza M, Goettert M, Geller F, Luik S, Flores EM, et al. Biological studies on Brazilian plants used in wound healing. J Ethnopharmacol 2009;122:523-32.

Al-Mutheffer EH. The effect of local application of blackseed (Nigella sativa) oil on wound healing in rabbits. Al Anbar J Vet Sci 2010;3:90-7.

Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M. Growth factors and cytokines in wound healing. Wound Repair Regen 2008;16:585-601.

Jorge MP, Madjarof C, Gois Ruiz AL, Fernandes AT, Ferreira Rodrigues RA, de Oliveira Sousa IM, et al. Evaluation of wound healing properties of arrabidaea chica verlot extract. J Ethnopharmacol 2008;118:361-6.

Al-Douri AS, Al-Kazaz Sgh A. The effect of Nigella sativa oil (black seed) on the healing of chemically induced oral ulcer in rabbit (experimental study). Al Rafidain Dent J 2010;10:151-7.

Ab Rahman MR, Abdul Razak F, Mohd Bakri M. Evaluation of wound closure activity of Nigella sativa, Melastoma malabathricum, Pluchea indica, and Piper sarmentosum extracts on scratched monolayer of human gingival fibroblasts. Evid Based Complement Alternat Med 2014;2014:190342.

Kirui PK, Cameron J, Benghuzzi HA, Tucci M, Patel R, Adah F, et al. Effects of sustained delivery of thymoqiunone on bone healing of male rats. Biomed Sci Instrum 2004;40:111-6.

Banks WJ. Applied Veterinary Histology. 3rd ed. Philadelphia: Sydney; 1993.

Allen AC. Skin. In: Kissane JM, editor. Anderson’s Pathology. 9th ed., Vol. 2. St. Louis: The C.V. Mosby Company; 1990.

Houghton PJ, Hylands PJ, Mensah AY, Hensel A, Deters AM. In vitro tests and ethnopharmacological investigations: Wound healing as an example. J Ethnopharmacol 2005;100:100-7.

Roy K, Shivakumar H, Sarkar S. Wound healing potential of leaf extracts of Ficus religiosa on wistar albino strain rats. Int J Pharmtech Res 2009;1:506-8.

Yaman I, Durmus AS, Ceribasi S, Yaman M. Efects of Nigella sativa and silver sulfadiazine on burn wound healing in rats. Vet Med 2010;55:619-24.

Aljabre SH, Alakloby OM, Randhawa MA. Dermatological effects of Nigella sativa. J Dermatol Dermatol Surg 2015;19:92-8.

Sari Y, Dhadhang WK, Nasruddin, Saryono, Arington IG, Toshio N. Formulation and evaluation of Nigella sativa oil gel for accelerating diabetic wound healing. Asian J Pharm Biol Res 2014;4:16-22.

Probst CW. Wound healing and specific tissue regeneration. In: Slatter D, editor. Text Book of Small Animal Surgery. 2nd ed., Vol. 1. Ch. 4. Philadelphia: W.B. Saunders Company; 1993. p. 53-63.

Song JJ, Salcido R. Use of honey in wound care: An update. Adv Skin Wound Care 2011;24:40-4.

Allwayzy KR. Effect of locally applied black-seed oil and honey mixture on wound healing. Int J Sci Technol Res 2013;2:31-4.

Ghedira K. La nigelle cultiv’ée: Nigella sativa L. (Ranunculaceae). Phytothérapie 2006;4:220-6.

Awad EM. In vitro decreases of the fibrinolytic potential of cultured human fibrosarcoma cell line, HT1080, by Nigella sativa oil. Phytomedicine 2005;12:100-7.

Ait Mbarek L, Ait Mouse H, Elabbadi N, Bensalah M, Gamouh A, Aboufatima R, et al. Anti-tumor properties of blackseed (Nigella sativa L.) extracts. Braz J Med Biol Res 2007;40:839-47.

Hosseinzadeh H. Anti-ischemic effect of Nigella sativa L. seed in male rats. Iran J Pharm Res 2006;5:53-8.

Abu-Zinadah OA. Using Nigella sativa oil to treat and heal wound of rabbit skin. JKAU Sci 2009;21:335-46.

Ostvar O, Shadvar S, Yahaghi E, Azma K, Fayyaz AF, Ahmadi K, et al. Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: A clinico-histopathologic study in rabbits. Diagn Pathol 2015;10:85.

Abu-Al-Basal MA. Influence of Nigella sativa fixed oil on some blood parameters and histopathology of skin in staphylococcal-infected BALB/c mice. Pak J Biol Sci 2011;14:1038-46.

Nayak S, Nalabothu P, Sandiford S, Bhogadi V, Adogwa A. Evaluation of wound healing activity of Allamanda cathartica. L. and Laurus nobilis. L. extracts on rats. BMC Complement Altern Med 2006;6:12.

Priya KS, Arumugam G, Rathinam B, Wells A, Babu M. Celosia argentea linn. Leaf extract improves wound healing in a rat burn wound model. Wound Repair Regen 2004;12:618-25.

Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 2003;17:299-305.

Sari Y, Dhadhang WK, Saryono, Arington IG, Nakatani T. The effect of Nigella sativa oil on wound healing: A pilot study. In: Proceedings of the 1st Borneo Nursing Conference. Pontianak, Indonesia; June, 2013.

Amin S, Mir SR, Kohli K, Ali B, Ali M. A study of the chemical composition of black cumin oil and its effect on penetration enhancement from transdermal formulations. Nat Prod Res 2010;24:1151-7.

Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000;14:323-8.

Fararh KM, Atoi Y, Shimizu Y, Shiino T, Nikomi H, Takewaki T. Mechanisms of the hypoglycaemic and immuno protectiating effects of Nigella sativa L., oil in streptozotocin induced diabetic hamsters. Res Vet Sci 2004;77:123-9.

Hashmi JT, Huang YY, Sharma SK, Kurup DB, De Taboada L, Carroll JD, et al. Effect of pulsing in low-level light therapy. Lasers Surg Med 2010;42:450-66.

Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR. The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 2012;40:516-33.

Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M. Low-level laser therapy for wound healing: Mechanism and efficacy. Dermatol Surg 2005;31:334-40.

Huang YY, Chen AC, Carroll JD, Hamblin MR. Biphasic dose response in low level light therapy. Dose Response 2009;7:358-83.

Peplow PV, Chung TY, Ryan B, Baxter GD. Laser photobiomodulation of gene expression and release of growth factors and cytokines from cells in culture: A review of human and animal studies. Photomed Laser Surg 2011;29:285-304.

Hashmi JT, Huang YY, Osmani BZ, Sharma SK, Naeser MA, Hamblin MR. Role of low-level laser therapy in neurorehabilitation. PM R 2010;2:S292-305.

Hawkins D, Houreld N, Abrahamse H. Low level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann N Y Acad Sci 2005;1056:486-93.

da Guarda MG, Paraguassú GM, Cerqueira NS, Cury PR, Farias JG, Ramalho LM. Laser gaAlAs (λ860 nm) photobiomodulation for the treatment of bisphosphonate-induced osteonecrosis of the jaw. Photomed Laser Surg 2012;30:293-7.

Bashardoust Tajali S, Macdermid JC, Houghton P, Grewal R. Effects of low power laser irradiation on bone healing in animals: A meta-analysis. J Orthop Surg Res 2010;5:1.

Hashmi JT, Huang YY, Osmani BZ, Sharma SK, Naeser MA, Hamblin MR. Role of low-level laser therapy in neurorehabilitation. PM R 2010;2:S292-305.

Al-Zamily RH. The influence of helium-neon laser irradiation on wound healing. Int J Pharm Bio Sci 2018;9:63-8.

Hawkins D, Abrahamse H. Biological effects of helium-neon laser irradiation on normal and wounded human skin fibroblasts. Photomed Laser Surg 2005;23:251-9.

Peccin MS, Renno AC, de Oliveira F, Giusti PR, Ribeiro DA. Helium- neon laser improves skin repair in rabbits. J Cosmet Laser Ther 2012;14:286-9.

Liao X, Xie GH, Liu HW, Cheng B, Li SH, Xie S, et al. Helium-neon laser irradiation promotes the proliferation and migration of human epidermal stem cells in vitro: Proposed mechanism for enhanced wound re-epithelialization. Photomed Laser Surg 2014;32:219-25.

Al-Zamily RH, Al-Siaidy WF. The effect of helium-neon laser, using different methods and frequencies of radiation, on wound healing. Int J Med Clin Res 2014;5:274.

Andrade Fdo S, Clark RM, Ferreira ML. Effects of low-level laser therapy on wound healing. Rev Col Bras Cir 2014;41:129-33.