FORMULATION AND EVALUATION OF TOPICAL HERBAL GEL CONTAINING INCLUSION COMPLEX OF CURCUMIN

VANDANA D*, SHWETA PAWAR

Authors

  • VANDANA D Department of Pharmaceutics, Gokaraju Rangaraju College of Pharmacy, Hyderabad, Telangana, India.
  • SHWETA PAWAR Department of Pharmaceutics, Gokaraju Rangaraju College of Pharmacy, Hyderabad, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i9.34053

Keywords:

Curcumin, Nil, Inclusion complex, Antimicrobial activity, Albumin protein denaturation, Anti-inflammation, ANOVA

Abstract

Objective: The current work was aimed to prepare a topical gel containing curcumin (CUR) for the treatment of microbial infections on skin.

Methods: CUR was complexed with the β-cyclodextrin (β-CD) using kneading method in 1:1, 1:2, and 1:3 molar ratios and characterized. The inclusion complex with high aqueous solubility was loaded in the topical gel containing (2% CUR) which was prepared using carbopol, sodium CMC, and guar gum and evaluated for viscosity, spreadability, extrudability, pH, drug content, and in vitro diffusion studies. The in vitro anti-inflammatory activity of the gel was performed by albumin protein denaturation technique, the statistical analysis was done using ANOVA followed Dunnett’s t-test. The antimicrobial activity of CUR was evaluated using standard strains of Candida albicans and Escherichia coli by agar well diffusion method.

Results: The complexation of CUR had an increased solubility up to 103.09 times for 1:3 molar ratio with in vitro dissolution 90.64% for 60 min. The optimized formulation F9 had viscosity of 6500.3 cps and 97.5% in vitro drug diffusion for 8 h which follows zero-order release kinetics. In vitro anti-inflammatory activity studied showed that the CUR gel has a good potency for renaturation and was as effective as standard diclofenac with 76.9% inhibition (p=0.0507). CUR showed approx. 3 mm diameter of zone of inhibition against C. albicans and E. coli.

Conclusion: A stable topical gel of CUR using β-CD and carbopol was successfully prepared which showed better in vitro diffusion with promising anti-inflammatory and antifungal action.

Downloads

Download data is not yet available.

References

Ansari MJ, Ahmed MM, Fatima F, Anwer MK, Jamil S, Al-Shdefat R. Solubility and stability enhancement of curcumin through cyclodextrin complexation. Int J Biol Pharm Allied Sci 2014;3:2668-75.

Suresh D, Chaudhari YS, Borole P, Mishra PT, Shenghani K, Duvvuri P. Degradation studies of curcumin. Int J Pharm Res Rev 2013;3:50-5.

Shaveta S, Shweta P, Upendra J. Development and evaluation of topical gel of curcumin from different combination of polymers formulation and evaluation of herbal gel. Int J Pharm Pharm Sci 2012;4:452-6.

Nikunjana A, Natvar J, Rakesh P. Formulation and evaluation of curcumin gel for topical application. Pharm Dev Technol 2009;14:83-92.

Nasreen S, Vijaya K. Prevention of peptic ulcers by curcumin in chemically induced osteoarthritis. Int J Pharm Pharm Sci 2018;10:29-34.

Thriveni VK, Manjunatha H, Ajesh K. Anti-inflammatory activity of curcumin and capsaicin augmented in combination. Int J Pharm Pharm Sci 2017;9:145-9.

Silverstein RM, Webster FX. Spectrometric Identification of Organic Compounds. 6th ed. Hoboken, New Jersey: Wiley; 2005. p. 79-109.

Yadav VR, Suresh S, Devi K, Yadav S. Effect of cyclodextrin complexation of curcumin on its solubility and antiangiogenic and anti-inflammatory activity in rat colitis model. AAPS PharmSciTech 2009;10:752-62.

Kalyan H, Ravi K, Biresh KS, Chowdary YA, Devgan M, Maddi R. UV-visible spectrophotometric estimation of curcumin in nanoformulation. Int J Pharmacogn 2015;2:127-30.

Raymond R, Paul J. Handbook of Pharmaceutical Excipients. 5th ed. Washington, DC: Pharmaceutical Press; 2006. p. 110-3, 298-300, 794-5.

Ramesh AR, Pathak AR, Sakarkar DM, Kunjwani HK, Suhas B, Patel J. Formulation and evaluation of thiocolchicoside topical gel by using different method. Int J Phytopharm 2016;6:27-30.

Kalita V, Pegu P, Chetia P. Phytochemical screening and evaluation of antioxidant, anti-microbial and anti-inflammatory activity of Curcuma caesia. Int J Pharm Sci Res 2019;10:846-55.

Niyaz B, Kalyani P, Divakar G. Formulation and evaluation of gel containing fluconazole-antifungal agent. Int J Drug Dev Res 2011;3:109-28.

Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with same biologically active proteins. J Pharm Pharmacol 1968;20:169-73.

Umme H, Shivakumar HG, Osmani RA, Srivastava A, Varma NS. Development of a curcumin bioadhesive monolithic tablet for treatment of vaginal candidiasis. Iran J Pharm Res 2016;15:23-34.

Published

07-09-2019

How to Cite

VANDANA D, and SHWETA PAWAR. “FORMULATION AND EVALUATION OF TOPICAL HERBAL GEL CONTAINING INCLUSION COMPLEX OF CURCUMIN: VANDANA D*, SHWETA PAWAR”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 9, Sept. 2019, pp. 196-01, doi:10.22159/ajpcr.2019.v12i9.34053.

Issue

Section

Original Article(s)