COMPARATIVE ESTIMATION OF SALIVARY TOTAL ANTIOXIDANT CAPACITY IN PERIODONTAL HEALTH AND CHRONIC PERIODONTITIS - A PILOT STUDY

RAGHAVENDRA U; ANUPAMA RAO; JYOTHI DESOZA; VINITA RAMANATH PAI; SINDHU NAIR; VIJAYA KUMAR; BHUVANESH SUKHLAL KALAL

doi:10.22159/ajpcr.2019.v12i5.34139

Authors

RAGHAVENDRA U Department of Biochemistry, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
ANUPAMA RAO Department of Periodontology, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
JYOTHI DESOZA Department of Biochemistry, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
VINITA RAMANATH PAI Department of Biochemistry, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
SINDHU NAIR Department of Biochemistry, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
VIJAYA KUMAR Department of Periodontology, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
BHUVANESH SUKHLAL KALAL Department of Biochemistry, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i5.34139

Keywords:

Total antioxidant capacity, Chronic periodontitis, Polymorphonuclear leukocyte, Reactive oxygen species, Free radicals, Oxidative stress, Antioxidants

Abstract

Objective: Gram-negative bacteria provoke polymorphonuclear leukocyte (PMN) to release reactive oxygen species in chronic periodontitis (CP). Inability to maintain a balance between oxidative stress and antioxidant levels makes patients more susceptible to periodontal disease. The present study aims to estimate and compare salivary total antioxidant capacity (TAOC) in subjects with clinically healthy periodontium and patients with CP.

Methods: After fulfilling the selection criteria, a total of 20 subjects (10 with clinically healthy periodontium and 10 with CP) were subjected to unstimulated salivary sample collection for biochemical estimation of TAOC by spectrophotometric assay using Kovacevic method. Analysis of data was done with unpaired student t-test, using SPSS version 22 statistical program.

Results: Salivary TAOC was significantly higher in subjects with clinically healthy periodontium compared to CP patients. It was statistically significant (p<0.001).

Conclusion: This study indicated increased levels of salivary TAOC in patients with CP compared to clinically healthy periodontium. Alteration in defensive antioxidant status could be a risk factor in the progression of periodontal disease.