COLOR INTENSITY AND ANTIOXIDATIVE CAPACITY OF FOUR NATURAL MONOFLORAL HONEY FROM MALDA, INDIA
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i9.34352Keywords:
Mango honey, Color intensity, IC50 value, Antioxidative capacity, Total phenolics content, Total flavonoids contentAbstract
Objective: The objective of the study was to explore the color intensity and antioxidative capacity of mango honey available from local niches, in and around Malda, West Bengal, India.
Methods: Four mango honey samples: LMH1, LMH2, LMH3, and LMH4, were collected from bee hives, in and around Malda, India, and were subjected to determine the color intensity and antioxidative capacity in 2, 2-diphenyl-1-picrylhydrazyl system.
Results: The honey samples tested had light to dark color variation with color intensity values of 583.59–1842.84 mAU. The total phenolic content (TPC) of the honey samples ranged from 434.52 to 1450.74 μg gallic acid equivalents/ml, while the total flavonoids content (TFC) ranged 39.4–72.67 mg quercetin equivalents/100 g of honey. The honey samples displayed antioxidative activity (AOA) with 9.91–76% inhibition capacity, and the IC50 values of 8.37–19.38 mg/ml. The color intensity of test honeys displayed positive correlation with the TPC (r=0.907) and TFC (r=0.863), while negative correlation between honey color intensity and IC50 values (r=−0.955) was recorded.
Conclusion: The locally available mango honeys were rich in bioactive components displaying excellent AOA, and thus, the native honey might be utilized as the potential source of natural antioxidant in our part of the globe.
Downloads
References
Anbudhasan P, Surendraraj A, Karkuzhali S, Sathishkumaran P. Natural antioxidants and its benefits. Int J Food Nutr Sci 2014;3:225-32.
Li Y, Hu K, Yu Y, Rotenberg SA, Amatore C, Mirkin MV, et al. Direct electrochemical measurements of reactive oxygen and nitrogen species in nontransformed and metastatic human breast cells. J Am Chem Soc 2017;139:13055-62.
Boukhenouna S, Wilson MA, Bahmed K, Kosmider B. Reactive oxygen species in chronic obstructive pulmonary disease. Oxid Med Cell Longev 2018;11:1-9.
Bouayed J, Bohn T. Exogenous antioxidants double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev 2010;3:228-37.
Mandal MD, Mandal S. Honey: Its medicinal property and antibacterial activity. Asian Pac J Trop Biomed 2011;1:154-60.
Pannu A, Parle M. Anti-obsessive-compulsive activity of honey. Asian J Pharm Clin Res 2017;10:206-9.
Chaiyasut C, Kesika P, Peerajan S, Sivamaruthi BS. The total polyphenolic content and antioxidant properties of various honey and sugars. Asian J Pharm Clin Res 2018;11:467-71.
Bogdanov S, Jurendic T, Sieber R, Gallmann P. Honey for nutrition and health: A review. J Am Coll Nutr 2008;27:677-89.
Özcan M, Juhaimi FA. Honey as source of natural antioxidants. J Apic Res 2015;54:145-54.
Arawwawala LD, Hewageegana HG. Health benefits and traditional uses of honey: A review. J Apictherapy 2017;2:9-14.
Alzahrani HA, Alsabehi R, Boukraâ L, Abdellah F, Bellik Y, Bakhotmah BA, et al. Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins. Molecules 2012;17:10540-9.
Ahmed M, Shafiq MI, Khaleeq A, Huma R, Qadir MA, Khalid A, et al. Physiochemical, biochemical, minerals content analysis, and antioxidant potential of national and international honeys in Pakistan. J Chem 2016;13:1-10.
Ahmed S, Sulaiman SA, Baig AA, Ibrahim M, Liaqat S, Fatima S, et al. Honey as a potential natural antioxidant medicine: An insight into its molecular mechanisms of action. Oxid Med Cell Longev 2018;2018:8367846.
Roodi PA, Moosavi Z, Goli AA, Azizzadeh M, Hosseinzadeh H. Histopathological study of protective effects of honey on subacute toxicity of acrylamide-induced tissue lesions in rats’ brain and liver. Iran J Toxicol 2018;12:1-8.
Stagos D, Soulitsiotis N, Tsadila C, Papaeconomou S, Arvanitis C, Ntontos A, et al. Antibacterial and antioxidant activity of different types of honey derived from mount Olympus in Greece. Int J Mol Med 2018;42:726-34.
Deng J, Liu R, Lu Q, Hao P, Xu A, Zhang J, et al. Biochemical properties, antibacterial and cellular antioxidant activities of buckwheat honey in comparison to Manuka honey. Food Chem 2018;252:243-9.
Sohaimy SA, Masry SH, Shehata MG. Physicochemical characteristics of honey from different origins. Ann Agric Sci 2015;60:279-87.
Kothai S, Jayanthi B. Evaluation of antioxidant and antimicrobial activity of stingless bee propolis (Tetragonula iridipennis) of Tamil Nadu, India. Int J Pharm Pharm Sci 2014;6:81-5.
Yap P, Bakar MF. Physicochemical, phytochemical and antimicrobial properties of wild honey collected at mangrove and mountain areas in Sabah, Malaysian Borneo. Int J Pharm Pharm Sci 2014;6:287-92.
Roy S, Mandal M, Pal NK, Das MK, Halder D, Sircar B, et al. Exploration of antibacterial and antioxidative property of two natural honey samples from Malda district, India. Transl Med 2016;6:1-5.
Beretta G, Granata P, Ferrero M, Orioli M, Facino RM. Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta 2005;533:185-91.
Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. J Pharm Belg 1994;49:462-8.
Habib HM, Ibrahim WH, Schneider-Stock R, Hassan HM. Camel milk lactoferrin reduces the proliferation of colorectal cancer cells and exerts antioxidant and DNA damage inhibitory activities. Food Chem 2013;141:148-52.
Saha A, Mandal S. In vitro assessment of two commercial honey samples for antibacterial and antioxidant activities. Austin J Trop Med Hyg 2015;1:1-5.
Bertoncelj J, Doberšek U, Korošec M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem 2007;105:822-8.
Islam A, Khalil I, Islam N, Moniruzzaman M, Mottalib A, Sulaiman SA, et al. Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year. BMC Complement Altern Med 2012;12:1-10.
Moniruzzaman M, Sulaiman SA, Khalil MI, Gan SH. Evaluation of physicochemical and antioxidant properties of sourwood and other Malaysian honeys: A comparison with manuka honey. Chem Cent J 2013;7:138.
Jasicka-Misiak I, Makowicz E, Stanek N. Chromatographic fingerprint, antioxidant activity, and colour characteristic of Polish goldenrod (Solidago virgaurea L.) honey and flower. Eur Food Res Technol 2018;244:1169-84.
Nweze JA, Okafor JI, Nweze EI, Nweze JE. Evaluation of physicochemical and antioxidant properties of two stingless bee honeys: A comparison with Apis mellifera honey from Nsukka, Nigeria. BMC Res Notes 2017;10:1-6.
Moloudian H, Abbasian S, Nassiri-Koopaei N, Tahmasbi MR, Afzal GA, Ahosseini MS, et al. Characterization and classification of Iranian honey based on physicochemical properties and antioxidant activities, with chemometrics approach. Iran J Pharm Res 2018;17:708-25.
Nayik GA, Nanda VA. Chemometric approach to evaluate the phenolic compounds, antioxidant activity and mineral content of different unifloral honey types from Kashmir, India. Food Sci Technol 2016;74:504-13.
Dezmirean GI, Mărghitaş LA, Bobiş O, Dezmirean DS, Bonta V, Erler S, et al. Botanical origin causes changes in nutritional profile and antioxidant activity of fermented products obtained from honey. J Agric Food Chem 2012;60:8028-35.
Saxena S, Gautam S, Sharma A. Physical, biochemical and antioxidant properties of some Indian honeys. Food Chem 2010;118:391-7.
Mahnot NK, Saikia S, Mahanta CL. Quality characterization and effect of sonication time on bioactive properties of honey from North East India. J Food Sci Technol 2019;56:724-36.
Ávila S, Hornung PS, Teixeira GL, Malunga LN, Apea-Bah FB, Beux MR, et al. Bioactive compounds and biological properties of Brazilian stingless bee honey have a strong relationship with the pollen floral origin. Food Res Int 2019;123:1-10.
Moniruzzaman M, An CY, Rao PV, Hawlader MN, Azlan SA, Sulaiman SA, et al. Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: Determination of antioxidant capacity. BioMed Res Int 2014;14:1-11.
El-Haskoury R, Kriaa W, Lyoussi B, Makni M. Ceratonia siliqua honeys from Morocco: Physicochemical properties, mineral contents, and antioxidant activities. J Food Drug Anal 2018;26:67-73.
Al-Farsi M, Al-Amri A, Al-Hadhrami A, Al-Belushi S. Color, flavonoids, phenolics and antioxidants of Omani honey. Heliyon 2018;4:e874.
Kamal MM, Rashid MH, Mondal SC, Taj HF, Jung C. Physicochemical and microbiological characteristics of honey obtained through sugar feeding of bees. J Food Sci Technol 2019;56:2267-77.
Khalil I, Moniruzzaman M, Boukraâ L, Benhanifia M, Islam A, Islam N, et al. Physicochemical and antioxidant properties of Algerian honey. Molecules 2012;17:11199-215.
Cruz LC, Batista JE, Zemolin AP, Nunes ME, Lippert DB, Royes LF, et al. A study on the quality and identity of Brazilian pampa biome honey: Evidences for its beneficial effects against oxidative stress and hyperglycemia. Int J Food Sci 2014;2:1-11.
Frankel S, Robinson GE, Berenbaum MR. Antioxidant capacity and correlation characteristics of 14 unifloral honeys. J Apic Res 1998;37:27-31.
Neupane BP, Malla KP, Kaundinnyayana A, Poudel P, Thapa R, Shrestha S. Antioxidant properties of honey from different altitudes of Nepal Himalayas. Pol J Food Nutr Sci 2015;65:87-91.
Mellen M, Fikselová M, Mendelová A, Haščík P. Antioxidant effect of natural honeys affected by their source and origin. Pol J Food Nutr Sci 2015;65:81-5.
Fikselová M, Kačániová M, Hleba L, Mellen M, Vukovič N, DžuganM. Antimicrobial and antioxidant activity of natural honeys of different origin. Anim Sci Biotechnol 2014;47:218-24.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
