BIOACTIVE COMPOUND ANALYSIS AND ANTIOXIDANT ACTIVITY OF ENDOPHYTIC BACTERIAL EXTRACT FROM ADHATHODA BEDDOMEI

Authors

  • Swarnalatha Y sathyabama university
  • Bhaswati Saha sathyabama university
  • Lokeswara Choudary y Nandanam arts college

Abstract

Objectives: The endophytic bacteria Lactobacillus sp. has been isolated from the tissues of plant leaves. The bacterial strains were grown in nutrient
broth at 37°C for 5 days. To isolate the bioactive compounds, the culture was grown in a broth and the culture broth was centrifuged and the
supernatant was collected.
Methods: Solvent-solvent method was applied to extract the bioactive compounds. The antioxidant activity was evaluated with the help of
1,1-diphenyl -2-picrylhydrazyl radical scavenging activity.
Results: The qualitative analysis of extracts indicated presence of carbohydrates, tannins, saponins, alkaloids, glycosides, proteins, amino acids, and
saponins. Flavonoids and anthocyanins were showed negative results, the quantitative analysis of the phenolic compounds showed the presence of
0.67 mg/ml. The inhibitory concentration 50 value for antioxidant activity was 35 μg/ml.
Conclusion: The antioxidant activity is due to the presence of different bioactive, and the high levels of phenolic compounds may be responsible for
antioxidant activity.

Keywords: Lactobacillus sp., Antioxidant activity, Endophytic bacteria, Phenolic compounds.

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Author Biography

Swarnalatha Y, sathyabama university

Department of biotechnology

References

Sturz AV, Christie BR, Nowak J. Bacterial endophytes: Potential role in

developing sustainable system of crop production. Crit Rev Plant Sci

;19:1-30.

Wellington B, Marcela TB. Delivery methods for introducing

endophytic bacteria into maize. BioControl 2004;49:315-22.

Hallmann J, Quadt-Hallmann A, Mahaffee WF, Kloepper JW. Bacterial

endophytes in agricultural crops. Can J Microbiol 1997;43(10):895- 914.

Buchenauer H. Biological control of soil-borne disease by rhizobacteria.

J Plant Dis Protec 1998;105(4):329-48.

Mclnroy JA, Kloepper JW. Survey of indigenous bacterial endophytes

from cotton and sweet corn. Plant Soil 1995;173(2):337-42.

Sturz AV, Christie BR, Matheson BG, Nowak J. Biodiversity of

endophytic bacteria potential role which colonize red clover nodules,

root, stem and foliage and their influence on host growth. Biol Fertil

Soil 1997;25:13-9.

Carmen A, Brandon R. Isolation and identification of endophytic

bacteria from roots of lemon grass (Cymbopogon citratus). Eur J Agric

Sci 2013;10:37-40.

Odebiyi OO, Sofowora EA. Phytochemical screening of Nigerian

medicinal plants II. Lloydia 1978;41:234-46.

Milan SS. Total phenolics content, flavonoid concentration and

antioxidant activity of Marrubium peregrinum L. extract. Kragujevac

J Sci 2011;33:63-72.

Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of

medicinal herbs from Ghana: Thonningia sanguinea on experimentallyinduced

liver injuries. Gen Pharmacol 1999;32:661-7.

Yuhernita Y, Juniarti J. Secondary metabolites analysis of methanol

extract of Surian (Toona sureni (Bl.) Merr) leaf as antioxidant potential.

Makara Sains 2011;15(1):48-52.

Baghiani A, Boumerfeg S, Belkhiri F, Khennouf S, Charef N,

Harzallah D, et al. Antioxidant and radical scavenging properties of

Carthamus caeruleus L. extracts grow wild in Algeria flora. Comun Sci

;1(2):128-36.

Khennouf S, Iratni N, Baghiani A, Harzallah D, Arrar L. Antioxidant

and antibacterial activities of extracts from Artemisia herba alba

Asso leaves and some phenolic compounds. J Med Plant Res

;4(13):1273-80.

Fernandes MR, Costa-Silva TA, Pfenning LH, Costa-Neto CM,

Heinrich TA, Alencar SM, et al. Biological activities of the fermentation

extract of the endophytic fungus Alternaria alternata isolated from

Coffea arabica L. Braz J Pharm Sci 2009;45(4):677- 85.

Scholz E, Rimpler H. Proanthocyanidins from Krameria triandra root.

Planta Med 1989;55(4):379-84.

Conde E, Moure A, Domínguez H, Parajó JC. Fractionation of

antioxidants from autohydrolysis of barley husks. J Agric Food Chem

;56:10651-9.

Strobel GA. Endophytes as sources of bioactive products. Microbes

Infect 2003;5(6):535-44.

Published

01-01-2015

How to Cite

Y, S., B. Saha, and L. Choudary y. “BIOACTIVE COMPOUND ANALYSIS AND ANTIOXIDANT ACTIVITY OF ENDOPHYTIC BACTERIAL EXTRACT FROM ADHATHODA BEDDOMEI”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 1, Jan. 2015, pp. 70-72, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/3457.

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