THE EFFECTS OF PHALERIA MACROCARPA EXTRACT ON MITOTIC INDEX AND TUMOR MASS PROGRESSION OF EPIDERMOID CARCINOMA IN SWISS MICE: IN VIVO EXPERIMENTAL STUDY IN SWISS MICE TREATED WITH NEOADJUVANT PACLITAXEL AND CISPLATIN: In vivo experimental study in Swiss mice treated with neoadjuvant paclitaxel and cisplatin

VITO MAHENDRA EKASAPUTRA; MUHAMAD THOHAR ARIFIN; DJOKO HANDOJO

doi:10.22159/ajpcr.2020.v13i1.34971

Authors

VITO MAHENDRA EKASAPUTRA Department of Surgery, Faculty of Medicine, Sultan Agung Islamic University Hospital, Semarang, Indonesia.
MUHAMAD THOHAR ARIFIN Department of Neurosurgery, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.
DJOKO HANDOJO Department of Surgery, Division of Surgical Oncology, Faculty of Medicine Diponegoro University, Semarang, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i1.34971

Keywords:

Phaleria macrocarpa, Mitotic index, Tumor diameter, Epidermoid carcinoma

Abstract

Objectives: Polyphenols in Phaleria macrocarpa (mahkota dewa) can inhibit mitogen-activated protein kinase activity in receptor tyrosine kinases pathway. This can be recognized from the decrease of mitotic index as a response to malignant cells and the reduction of tumor development. This study aimed to determine whether P. macrocarpa may decrease the mitotic index and tumor diameter in epidermoid carcinoma.

Methods: The experiment was conducted on 18 epidermoid carcinoma induced Swiss mice divided into four groups: Control, Phaleria administered (0.0715 mg [0.36 ml]/day), chemotherapy administered (paclitaxel 175 mg/m2 and cisplatin 50 mg/m2), and combination group. Tumor size diameter was measured before and after treatment in 9 weeks. Mitotic index was measured at the end of the treatment.

Results: There were significant differences in the mitotic index and changes in tumor diameter among groups compared with the control group. The most significant growth inhibition and decrease in mitotic index were in group four. There was a significant positive correlation between tumor mitotic index and changes in tumor diameter (r=0.813).

Conclusion: P. macrocarpa is able to decrease tumor cells’ mitotic index and inhibit epidermoid carcinoma’s tumor mass progression in Swiss mice.

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References

Nouri K. Skin Cancer: Squamous Cell Carcinoma of the Skin. New York: McGraw Hill; 2008. p. 86-110.

American Cancer Society. Cancer Facts and Figure; 2003. Available from: http://www.cancer.org/downloads/STT/CAF.secured.pdf.

Pasaribu ET, Suryatno S, Riefmanto BH. Bedah Onkologi Diagnosis Dan Terapi. 1st ed. (Original article in Indonesian). Jakarta: Sagung Seto; 2010.

Thompson AK, Kelley BF, Prokop LJ, Murrad MH, Baum CL. Risk factors for cutaneous squamous cell carcinoma outcomes: A systematic review and meta-analysis. JAMA Dermatol 2016;152;419-28.

Gniadecki R. Non Surgical Treatment of Keratinocyte Skin Cancer- Systemic Chemotherapy of Non Melanoma Skin Cancer. Berlin: Springer-Berlin; 2010.

Baretta G. Cancer Treatment Medical Guide. 11th ed. Milan: Farmitalia Carlo Erba Erbamont; 2001. p. 174-6.

Winarto WP. Mahkota Dewa: Budi Daya dan Pemanfaatan Untuk Obat (Original article in Indonesian). Jakarta: Penebar Swadaya; 2004.

Tjandrawinata R, Djunarko I, Fenty F, Hendra P. Anti-inflammation effects of bioactive fraction DLBS0533 containing Phaleria macrocarpa and Nigella sativa on animal model. Int J Pharm Pharm Sci 2015;7:408-11.

Tjandrawinata R, Rouli H. A role for Phaleria macrocarpa (Scheff Boerl) extracts in the management of women’s pathological conditions: A research review. Int J Pharm Pharm Sci 2017;9:7-12.

Lisdawati V. Phaleria macrocarpa, Toksisitas, Efek Anti Oksidan dan Efek Anti Kanker Berdasarkan Uji Penapisan Farmakologi (Original article in Indonesian). Jakarta: PT Mahkota Dewa; 2002.

Sumastuti R, Sonlimar M. Efek Sitotoksik Ekstrak Buah dan Daun Mahkota Dewa (Phaleria macrocarpa) Scheff Boerl Terhadap Sel Hela (Original article in Indonesian). Yogyakarta: Farmakologi FK UGM; 2003.

Faried A, Kurnia D, Faried LS, Usman N, Miyazaki T, Kato H, et al. Anti cancer effects of gallic acid isolated from Indonesian herbal medicine, Phaleria macrocarpa (Scheff) boerl on human cancer cell lines. Int J Oncol 2007;30:605-13.

Deguchi H, Fujii T, Nakagawa S, Koga T, Shirouzu K. Analysis of cell growth inhibitory effect of cathecin through MAPK in human breast cancer cell line T47D. Int J Oncol 2002;21:1301-5.

Ahmad N, Muhtar H. Green Tea Polyphenols and Cancer: Biologic Mechanism and Practical Implication. Cleveland: University Hospital of Cleveland; 1999. p. 78-83.

Budijitno S. Pengaruh Ekstrak Phaleria macrocarpa Terhadap Skor Ekspresi Perforin CTL dan Sel-NK Serta Indek Apoptosis pada Adenokarsinoma Mammae Mencit C3H. Vol. 42. (Original article in Indonesian) Majalah MMI; 2007. p. 13-9.

Estuningtyas A, Widiasari S, Kusmardi K. Acute toxicity of chitosan nanoparticles containing mahkota dewa (Phaleria macrocarpa) leaf extract and anti-inflammatory effects in a dextran sodium sulfate-induced mouse model of ulcerative colitis. Int J Appl Pharm 2018;10:6-10.