PRELIMINARY PHYTOCHEMICAL EVALUATION AND SPECTRAL CHARACTERIZATION OF ACTIVE CONSTITUENTS IN THE DRIED EXTRACTS OF THE WHOLE PLANT ARGYREIA IMBRICATA (ROX) SANT AND PATEL

SEBASTIN V; GOPALAKRISHNAN G; SREEJITH M; ANOOB KUMAR KI

doi:10.22159/ajpcr.2019.v12i11.35464

Authors

SEBASTIN V Department of Pharmaceutical Chemistry, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India.
GOPALAKRISHNAN G Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.
SREEJITH M Department of Pharmaceutical Chemistry, Nazareth College of Pharmacy, Pathanamthitta, Kerala, India.
ANOOB KUMAR KI Department of Pharmaceutical Chemistry, KVM College of Pharmacy, Alappuzha, Kerala, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i11.35464

Keywords:

Argyreia imbricata, Preliminary phytochemical evaluation, Chromatographic separation, Spectral evaluation

Abstract

Objective: The present study was designed with the aim of preliminary phytochemical evaluation and spectral characterization phytochemicals present in the extracts of whole plant Argyreia imbricata (Rox) Sant and Patel.

Methods: The whole plant A. imbricata was collected, authenticated, and dried in the shade for powdering in mechanical grinder. The powdered plant material was extracted with Soxhlet apparatus using different solvents, and the dried extract obtained was subjected to preliminary phytochemical evaluation, and then column chromatography separation and the separated fractions were subjected to thin-layer chromatography (TLC) separation. Based on the yield in TLC, the selected compounds were subjected to carbon-13 nuclear magnetic resonance, proton NMR (1H NMR), and mass spectroscopic evaluation.

Results: In the preliminary phytochemical evaluation, the presence of alkaloids, glycosides, phenolic compounds, flavonoids, carbohydrates, saponins, sterols, and terpenoids was found in different extracts. In column chromatography separation, totally 650 fractions were collected, and four compounds were isolated with the help of TLC. Among the four, two compounds were selected for spectral evaluation based on the yield obtained. The selected compounds were identified and named as, 3-ethyl-5-(hydroxymethyl) phenol and methyl 4-hydroxybenzoate with the help of spectral evaluation.

Conclusion: The outcome of this study is beneficial, and further investigation in the future directed toward the detailed pharmacological screening of the extracts may give more valuable results.

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Author Biographies

SEBASTIN V, Department of Pharmaceutical Chemistry, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India.

Asst. Prof., Dept. of Pharmacology

GOPALAKRISHNAN G, Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.

Associate Professor, Department of Pharmaceutical Chemistry, Malik Deenar College of Pharmacy, Seethangoli, Kasaragod, Kerala, India

SREEJITH M, Department of Pharmaceutical Chemistry, Nazareth College of Pharmacy, Pathanamthitta, Kerala, India.

Assistant Professor, Department of Pharmacy, Annamalai University, Chidambaram, Tamil Nadu, India

ANOOB KUMAR KI, Department of Pharmaceutical Chemistry, KVM College of Pharmacy, Alappuzha, Kerala, India.

Professor and Head, Department of Pharmaceutical chemistry, Nazareth College of Pharmacy, Thiruvalla, Pathanamthitta District, Kerala, India

References

Kunle OF, Egharevba HO, Ahmadu PO. Standardization of herbal medicines a review. Int J Biodivers Conserv 2012;4:101-12.

Haseebur R, Nazeen R, Mir H, Riaz M. Antioxidant and anti-inflammatory potentials of Solanum pubescens Willd an ethno medicinal plant of South Western Andhra Pradesh. J Res Pharm 2019;23:187-97.

Sarita M, Shisir L, Kumar D. In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. J Trop Med 2019;2019:5.

Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by paliyar tribe from Tamil Nadu, India. BMC Complement Altern Med 2006;6:35.

Tasnuva S, Sukla S. Screening of biological activities of Cucumis sativus leaf, growing in Bangladesh. J Pharmacogn Phytochem 2017;6:254-7.

Pushpa R, Mamta A, Subhanshi S. Phytochemical and antioxidant properties of various extracts of Michelia champaca leaves. Int J Pharm Pharm Sci 2019;11:56-61.

Balabhaskar R, Kumar AR, Selvarajan S, Faridha A, Gayathri G. Potential natural products with anticancer properties and their applications. Asian J Pharm Clin Res 2019;12:27-33.

Kumar U, Kumar B, Bhandari A, Kumar Y. Phytochemical investigation and comparison of antimicrobial screening of clove and cardamom. Int J Pharm Sci Res 2010;1:138-47.

Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. For in vitro antioxidant activities. Asian Pac J Trop Biomed 2014;4:S359-67.

Rahman G, Syed UJ, Syed F, Samiullah S, Nusrat J. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. Sci World J 2017;2017:7.

Gayathri S, Dhevi VS, Saranya S, Lokesh R. Phytochemical profiles, in vitro antioxidant, anti inflammatory and antibacterial activities of Terminalia catappa. Int J Pharm Sci Rev Res 2019;55:51-9.

Maruthamuthu R, Maithili V, Arunazhagi R, Yogesh V. Phytochemical profiling of medically significant crude extract using GC-MS analysis. Int J Curr Pharm Res 2018;10:16-20.

Harun AR, Deepak BP, Sayani M, Vivekananda M, Mahadeb P, Subhash CM, et al. Antioxidant and anticancer activity of extract and fractions obtained from Diospyros melanoxylon Roxb. leaves and correlation with their polyphenolic profiles. Int J Pharm Pharm Sci 2018;10:6-16.