Safety evaluation of petroleum ether, acetone, and hydroalcoholic extracts of Holoptelea integrifolia by oral administration in Wistar rats

Authors

  • Kumar D RMRC ICMR, BELGAUM, KARNATAKA, INDIA
  • Patil PA Department of Pharmacology, USM-KLE, Belgaum, Karnataka, India 590010
  • Harsha V Hegde Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010
  • Roy S Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010
  • Kholkute SD Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

Abstract

 

Objective: There are no reports on safety information of Holoptelea integrifolia (Roxb.) Planch. bark extracts by oral administration for long duration.
This study is planned to add information on the safety margin of H. integrifolia.
Methods: Acute oral toxicity study was carried out in the consideration of OECD guideline 425. Animals were observed for mortality, morbidity,
behavior and toxic symptoms for 14 consecutive days. Sub-acute toxicity was planned by employing the OECD guideline 407. Dose 50, 100
and 200 mg/kg of the extracts were administered orally, daily for 28 consecutive days. Animals were observed for toxicity sign, morbidity and
mortality. Body weight, relative organ weight, biochemical, hematological parameters and histopathology of vital organs like liver and kidney
were evaluated.
Results: In the acute oral toxicity study, all the extracts were found to be safe at 1000 mg/kg. However, out of three extracts, petroleum ether extract
of the plant showed mild damage to liver and kidney in sub-acute toxicity studies.
Conclusion: Hydroalcoholic extract of H. integrifolia is found to be safe, compared to the acetone and petroleum ether extracts for the long-term use.

Keywords: Holoptelea integrifolia, Bark, Acute toxicity, Chronic toxicity

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Author Biographies

Kumar D, RMRC ICMR, BELGAUM, KARNATAKA, INDIA

Senior research fellow, Department of experimental pharmacology, RMRC ICMR, Belgaum, Karnataka

Patil PA, Department of Pharmacology, USM-KLE, Belgaum, Karnataka, India 590010

Professor and Head, Department of Pharmacology, USM-KLE, Belgaum, Karnataka, India 590010

Harsha V Hegde, Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

 Scientist C, Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

Roy S, Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

Scientist E, Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

Kholkute SD, Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

Scientist G, Regional Medical Research Centre (ICMR), Belgaum, Karnataka, India- 590010

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Published

01-03-2015

How to Cite

Kumar D, Patil PA, H. V. Hegde, Roy S, and Kholkute SD. “Safety Evaluation of Petroleum Ether, Acetone, and Hydroalcoholic Extracts of Holoptelea Integrifolia by Oral Administration in Wistar Rats”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 2, Mar. 2015, pp. 185-9, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/3582.

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