A NOVEL REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF ALOE-EMODIN AND PIPERINE IN AYURVEDIC FORMULATION

Authors

  • LEENA TANDEL Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
  • REVATI SONONE Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
  • VANDANA JAIN Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i2.36326

Keywords:

Aloe-emodin, Piperine, Ayurvedic formulation, Reverse-phase high-performance liquid chromatographic, Validation

Abstract

Objective: The objective of this study was to develop and validate a novel, simple, rapid, accurate, and precise reverse-phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of aloe-emodin and piperine in the ayurvedic formulation as per the International Conference on Harmonization guidelines.

Methods: Chromatographic separation was achieved using a Prontosil C18 (250 × 4.6 mm, 5 μ), with a mobile phase consisting of 0.05% orthophosphoric acid and acetonitrile in the ratio of 50:50, at a flow rate of 1 ml/min and column temperature maintained at 28°C and ultraviolet (UV) detection at 225 nm.

Results: The retention time of aloe-emodin and piperine was found to be 9.38±0.2 min and 13.45±0.2 min, respectively. The linearity of aloe-emodin and piperine was tested in the range of 1–20 μg/ml. The correlation coefficient for aloe-emodin and piperine was found to be 0.998 and 0.997, respectively. The recovery values (98–102%) indicate a satisfactory accuracy. The percentage relative standard deviation for precision was found to be <2% which indicates that the method is precise.

Conclusion: A precise method for the simultaneous quantification of aloe-emodin and piperine was developed using high-performance liquid chromatography. Hence, the developed method can be used for quantitative and quality control analysis of formulations containing these phytoconstituents.

Downloads

Download data is not yet available.

Author Biography

LEENA TANDEL, Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

Associate Professor at oriental college of pharmacy, recipient of ugc-jrf in engg and tech.....successfully completed 2 minor research project granted by mumbai university.......several publications in national and international journal of good impact factor...

References

Pandey MM, Rastogi S, Rawat AK. Indian traditional Ayurvedic system of medicine and nutritional supplementation. Evid Based Complement Alternat Med 2013;2013:1-12.

Shaikh H, Jain V. A novel, simple, rapid RP-HPLC method for simultaneous estimation of ferulic acid, quercetin, piperine and thymol in Ayurvedic formulation. Int J App Pharm 2018;10:303-8.

Ong ES. Extraction methods and chemical standardization of botanicals and herbal preparations. J Chromatogr B Analyt Technol Biomed Life Sci 2004;812:23-33.

Narayanan S, Jadhav A. Simultaneous estimation of aloe emodin and emodin from Rheum emodi, Cassia alata and aloes by HPTLC. Indian J Pharm Sci 2015;77:783-7.

Vasavirama K, Upender M. Piperine: A valuable alkaloid from piper species. Int J Pharm Pharm Sci 2014;6:34-8.

Shaikh S, Jain V. A novel RP-HPLC method for simultaneous estimation of ellagic acid, quercetin and piperine in Ayurvedic formulations. Asian J Pharma Clin Res 2018;11:312-7.

Shah U, Jasani A. UV spectrophotometric and RP-HPLC methods for simultaneous estimation of isoniazid, rifampicin and piperine in pharmaceutical dosage form. Int J Pharm Pharm Sci 2014;6:274-80.

Bajad S, Singla AK, Bedi KL. Liquid chromatographic method for determination of piperine in rat plasma: Application to pharmacokinetics. J Chromatogr B Anal Technol Biomed Life Sci 2002;776:245-9.

Jain V, Shaikh S. Development and validation of a RP-HPLC method for the simultaneous determination of curcumin, piperine and camphor in an Ayurvedic formulation. Int J Pharm Pharm Sci 2018;10:115-21.

Tabin S, Gupta RC, Bansal G, Kamili AN. Comparative HPLC analysis of emodin, aloe emodin and rhein in Rheum emodi of wild and in vitro raised plants. J Pharmacogn phytochem 2016;5:121-30.

Mandrioli R, Mercolini L, Ferranti A, Fanali S, Raggi AM. Determination of aloe emodin in Aloe vera extracts and commercial formulations by HPLC with tandem UV absorption and fluorescence detection. Food Chem 2011;126:387-93.

Cock I, Ruebhart D. High performance liquid chromatographic separation and identification of a toxic fraction from Aloe barbadensis miller leaf gel using the Artemia nauplii bioassay. Int J Toxicol 2007;4:1-7.

International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. Validation of Analytical Procedures: Text and Methodology ICH Q2 (R1); 2005.

Published

07-02-2020

How to Cite

TANDEL, L., R. SONONE, and V. JAIN. “A NOVEL REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF ALOE-EMODIN AND PIPERINE IN AYURVEDIC FORMULATION”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 2, Feb. 2020, pp. 51-54, doi:10.22159/ajpcr.2020.v13i2.36326.

Issue

Section

Original Article(s)

Most read articles by the same author(s)