NEUROPROTECTIVE EFFECT OF POLYHERBAL FORMULATION IN PARKINSON’S ANIMAL MODEL

ANITHA NANDAGOPAl; MIR AMJAD ALI KHAN

doi:10.22159/ajpcr.2020.v13i3.36549

Authors

ANITHA NANDAGOPAl Department of Pharmacology, Sultan-ul-Uloom College of Pharmacy, Hyderabad, Telangana, India.
MIR AMJAD ALI KHAN Department of Pharmacology, Sultan-ul-Uloom College of Pharmacy, Hyderabad, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i3.36549

Keywords:

Polyherbal formulation, Prunus amygdalus, Arachis hypogaea, Citrullus lanatus, Nil, Dopamine, Antioxidants

Abstract

Objective: The study is aimed to evaluate the antiparkinsonian effect of polyherbal formulation containing methanolic extract of Prunus amygdalus, Arachis hypogaea, and Citrullus lanatus (MEPAC) in chlorpromazine (CPZ)-induced Parkinson’s disease in Wistar albino rats.

Methods: The antiparkinsonian activity of polyherbal formulation was studied in CPZ (3 mg/kg i.p.) induced Parkinson rat model. Rats were subjected to treatment with MEPAC and standard drug for a period of 21 days. The behavioral assessments, i.e., catalepsy and locomotor activity were assessed during the treatment period. Then animals were sacrificed, brains were isolated and homogenized for the estimation of biochemical parameters such as dopamine (DA), lipid peroxidation (LPO), glutathione (GSH), and superoxide dismutase (SOD). Histopathology of the brains was also done.

Results: The cataleptic score of MEPAC (200 mg/kg and 400 mg/kg) treated rats was significantly reduced. On the other hand, there was improved in the locomotor activity. MEPAC (200 mg/kg and 400 mg/kg) treated rats showed increase in the level of DA, reduced GSH, SOD, and decreased LPO significantly.

Conclusion: It may be concluded that methanolic extract of polyherbal formulation consisting of P. amygdalus, A. hypogaea, and C. lanatus showed a good antioxidant and neuroprotective effect in CPZ-induced Parkinson rats.

Downloads

Download data is not yet available.

Author Biographies

ANITHA NANDAGOPAl, Department of Pharmacology, Sultan-ul-Uloom College of Pharmacy, Hyderabad, Telangana, India.

Dept. of Pharmacology, Professor.

MIR AMJAD ALI KHAN, Department of Pharmacology, Sultan-ul-Uloom College of Pharmacy, Hyderabad, Telangana, India.

Dept. of Pharmacology

References

Lalotra S, Vaghela JS. Scientific reports of medicinal plants used for the prevention and treatment of neurodegenerative diseases. Pharm Biosci J 2019;7:15-25.

Tysnes OB, Storstein A. Epidemiology of Parkinson’s disease. J Neural Transm (Vienna) 2017;124:901-5.

Tanner CM, Goldman SM. Epidemiology of Parkinson’s disease. Neurol Clin 1996;14:317-35.

Chandra S, Srivastav M, Chauhan N. Recent advances in Parkinson disease. Pharm Biol Eval 2017;4:141-7.

Yadav HP, Li Y. The development of treatment for Parkinson’s disease. Adv Parkinsons Dis 2015;4:59-78.

Hatano T, Hattori N. Etiology and pathogenesis of Parkinson’s disease. In: Rana AQ, editors. Etiology and Pathophysiology of Parkinson’s Disease. Rijeka, Croatia: InTech; 2011. p. 1-6.

Stefanoni G, Sala G, Tremolizzo L, Brighina L, Ferrarase C. Alpha-synuclein, oxidative stress and autophagy failure: Dangerous liaisons in dopaminergic neurodegeneration. In: Rana AQ, editors. Etiology and Pathophysiology of Parkinson’s Disease. Rijeka, Croatia: InTech; 2011. p. 111-36.

Jankovic J. Parkinson’s disease: Clinical features and diagnosis. J Neurol Neurosurg Psychiatry 2008;79:368-76.

Ridolfi B, Abdel-Haq H. Neurodegenerative disorders treatment: The MicroRNA role. Curr Gene Ther 2017;17:327-63.

Isfahlan AJ, Mahmoodzadeh A, Hassanzadeh A, Heidari R, Jamai R. Anti-oxidant and anti radical activities of the phenolic extracts from Iranian almond (Prunus amygdalus) hulls and shells. Turk J Biol 2010;34:165-73.

Yadav DN, Yogesh K, Aswani A. Antioxidant activity of peanut (Arachis hypogaea L.) skin extract: Application in soybean and mustard oil. Int J Food Process Technol 2014;1:26-31.

Onyeso GI, Nkpaa KW, Osaretin PC, Ugochukwu E. Ameliorative effect of hydromethanolic fraction of Citrullus lanatus seeds on biochemical and histology parameters of female wistar rats administered with caffeine. Am Chem Sci J 2016;10:1-10.

Gill NS. Evaluation of antioxidant activity of Citrullus lanatus seed extract in rats. Lat Am J Pharm 2011;30:429-34.

Khandelwal KR. Preliminary phytochemical screening. In: Khandelwal KR, editors. Practical Pharmacognosy Techniques and Experiments. 14th ed. Pune: Nirali Prakashan; 2005. p. 149-56.

Bais S, Gill NS, Kumar N. Neuroprotective effect of Juniperus communis on chlorpromazine induced Parkinson disease in animal model. Chin J Biol 2015;2015:1-7.

Khisti RT, Mandhane SN, Chopde CT. Haloperidol-induced catalepsy: A model for screening antidepressants effective in treatment of depression with Parkinson’s disease. Indian J Exp Biol 1997;35:1297-301.

Shireen E, Haleem DJ. Reversal of haloperidol-induced motor deficits by mianserin and mesulergine in rats. Pak J Pharm Sci 2011;24:7-12.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.

Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170-5.

Manikkoth S, Deepa B, Sequeira M, Joy AE, Rodrigues R. Assessment of brain dopamine levels to evaluate the role of Tylophora indica ethanolic extract on alcohol induced anxiety in wistar albino rats. J Young Pharm 2016;8:91-5.

Parikh V, Khan MM, Mahadik SP. Differential effects of antipsychotics on expression of antioxidant enzymes and membrane lipid peroxidation in rat brain. J Psychiatr Res 2003;37:43-51.

Bishnoi M, Kumar A, Chopra K, Kulkarni SK. Comparative neurochemical changes associated with chronic administration of typical and atypical neuroleptics: Implications in tardive dyskinesia. Indian J Exp Biol 2007;45:175-9.

Sandhu KS, Rana AC. Evaluation of anti Parkinson’s activity of Nigella sativa (Kalonji) seeds in chlorpromazine induced experimental animal model. Int J Pharm Pharm Sci 2013;5:884-8.

Paradkar PP, Sarawade RD. Neuropharmacological evaluation of Brassica nigra seed extract in Parkinson’s disease. World J Pharm Res 2015;4:2291-308.

Schmidt N, Ferger B. Neurochemical findings in the MPTP model of Parkinson’s disease. J Neural Transm (Vienna) 2001;108:1263-82.

Bernheimer H, Birkmayer W, Hornykiewicz O, Jellinger K, Seitelberger F. Brain dopamine and the syndromes of Parkinson and huntington. Clinical, morphological and neurochemical correlations. J Neurol Sci 1973;20:415-55.

Dhingra D, Gahalain N. Amelioration of haloperidol-induced orofacial dyskinesia and catalepsy by ellagic acid in rats. Int J Res Ayurveda Pharm 2016;7:222-7.

Bhangale JO, Acharya SR. Anti-parkinson activity of petroleum ether extract of Ficus religiosa (L.) leaves. Adv Pharmacol Sci 2016;2016:9436106.

Ittiyavirah SP, Ruby R. Effect of hydro-alcoholic root extract of Plumbago zeylanica L alone and its combination with aqueous leaf extract of Camellia sinensis on haloperidol induced parkinsonism in wistar rats. Ann Neurosci 2014;21:47-50.

Nagarjuna S, Arifullah M, Kumar AS, Srinath B, Reddy KS, Reddy YP. Evaluation of antioxidant and antiparkinsonian activities of Brassica oleracea in haloperidol-induced tardive dyskinesia. Int J Green Pharm 2015;9:143-9.