HYDROGEL FORMULATION FROM CALOTROPIS GIGANTEA PLANT EXTRACT AGAINST FOOT ULCER CAUSING BACTERIA IN DIABETES

RAMACHANDRAN AM; SENTHIL PRABHU S; DEVAKUMAR J; RENGARAMANUJAM J; KARTHIK SUNDARAM S; MAHENTHIREN R; VARSHA TK

doi:10.22159/ajpcr.2021.v14i11.37147

Authors

RAMACHANDRAN AM Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
SENTHIL PRABHU S Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
DEVAKUMAR J Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
RENGARAMANUJAM J Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
KARTHIK SUNDARAM S Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
MAHENTHIREN R Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
VARSHA TK Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i11.37147

Keywords:

Diabetes mellitus, Calotropis gigantea, Hydrogels, Formulation, Hydrogel

Abstract

Objective: The objective of present study was to develop hydrogel formulations loaded with Calotropis gigantea leaves extract.

Methods: The prepared hydrogel formulations were compliance with their color, odor, homogeneity, pH, and spreadability.

Results: As all the formulations were complies with the all parameters and it can be suggest as a Good antibacterial gel. It is evident that, ethanolic extract of Calotropis gigantea showed a maximum inhibitory zone against bacteria associated with foot ulcer.

Conclusions: The In vitro studies showed a quantity dependent increase in antibacterial activity against foot ulcer causing bacteria, a contraction which is higher than that produced by the control groups. These contractions were statistically significant (p<0.05), during the study with leave extract against foot ulcer causing bacteria in diabetes.

Downloads

Download data is not yet available.

References

Jneid J, Lavigne J, La Scola B, Cassir N. The diabetic foot microbiota: A review. Hum Microbiome J 2017;5:1-6. DOI: https://doi.org/10.1016/j.humic.2017.09.002

Kumar G, Karthik L, Rao KV. Antibacterial activity of aqueous extract of Calotropis gigantea leaves-an in vitro study. Int J Pharm Sci Rev Res 2010;4:141-2.

Eziuzo OS, Amarauche C. Studies on sida acuta hydrogel I: Processing and physicochemical properties of the derived hydrogel obtained from South East Nigeria. IJPPS 2017;9:5-11. DOI: https://doi.org/10.22159/ijpps.2017v9i6.10097

Mikail HG. Phytochemical screening elemental analysis and acute toxicity of aqueous extract of Allium satiivum L. Bulb in experimental rabbits. J Med Plant Res 2010;4:322-6.

Kalaiselvi V, Binu TV, Radha SR. Preliminary phytochemical analysis of the various leaf extracts of Mimusops Elengi L. South Indian J Biol Sci 2016;2:24-9. DOI: https://doi.org/10.22205/sijbs/2016/v2/i1/100337

Edy HJ, Marchaban, Wahyuono S, Nugroho AE. Formulation and evaluation of hydrogel containing Tagetes erecta L. leaves ethanolic extract. Int J Curr Innov Res 2017;3:627-30.

Manivannan R, Shopna R. Antidiabetic activity of calotropis gigantea white flower extracts in alloxan induced diabetic rats. J Drug Deliv Ther 2017;7:106-11. DOI: https://doi.org/10.22270/jddt.v7i3.1447

Shobowale OO, Ogbulie NJ, Itoandon EE, Oresegun MO, Olatope SOA. Phytochemical and antimicrobial evaluation of aqueous and organic extracts of calotropis procera A it leaf and latex. NIFOJ 2013;31:77-82. DOI: https://doi.org/10.1016/S0189-7241(15)30059-X

Ogbulie JN, Ogueke CC, Nwanebu FC. Antibacterial properties of Uvaria chamae, Gongronema latifolium, Garcinia kola, vemonia amygdalina and aframomium melegueta. Afr J Biotechnol 2007;13:1549-53.

Shah MD, Iqbal M. Antioxidant activity, phytochemical analysis and total polyphenolics content of Essential oil, methanol extract and methanol fractions from commelina nudiflora. Int J Pharm Pharm Sci 2018;10:36-43. DOI: https://doi.org/10.22159/ijpps.2018v10i8.25922

Sharma M, Tandon S, Aggarwal V, Bhat KG, Kappadi D, Chandrashekhar P, et al. Evaluation of antibacterial activity of Calotropis gigantea against Streptococcus mutans and Lactobacillus acidophilus: An in vitro comparative study. J Conserv Dent 2015;18:457-60. DOI: https://doi.org/10.4103/0972-0707.168809

Patil SV, Roshan R, Mane RR. Bacterial and clinical profile of diabetic foot ulcer using optimal culture techniques. Int J Res Med Sci 2017;5:496-502. DOI: https://doi.org/10.18203/2320-6012.ijrms20170139

Bansal E, Garg A, Bhatia S, Attri AK, Chander J. Spectrum of microbial flora in diabetic foot ulcers. Indian J Pathol Microbiol 2008;51:204. DOI: https://doi.org/10.4103/0377-4929.41685