BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM MANGROVE BARK – RHIZOPHORA MUCRONATA EXTRACT
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i7.37634Keywords:
Biosynthesis, Rhizophora mucronata, Silver nanoparticles, HRTEM, Nil, EDX analysis, Dynamic light scatteringAbstract
Objective: The present study attempted to synthesize AgNPs from mangrove bark Rhizophora mucronata and analyze characteristics. The synthesized AgNPs analyzed with UV–vis spectroscopy, high-resolution transmission electron microscopy (HRTEM), and energy-dispersive X-ray (EDX) for confirming the nanoparticles.
Methods: The dried R. mucronata bark was powdered and kept in at 55°C for 15 min in a water bath and cooled at room temperature to get the extract. The R. mucronata bark extract was treated with silver nitrate and kept overnight in the dark environment which will turn the solution to dark brown color. The silver nanoparticles were characterized using UV–visible absorption at room temperature. Further characterization was also done with X-ray diffraction, high-resolution transmission electron microscope measurements, and DLS analysis.
Results: The synthesized AgNPs were analyzed with various analytical methods that revealed the abundant presence of silver nanoparticles. The UV–vis spectroscopy analysis exposed the surface plasmon resonance peak of 422 nm. High-resolution transmission electron microscopy (HRTEM) analysis indicated the size ranging from 10 nm to 200 nm in diameter and a spherical shaped poly dispersal of the particles. The energy-dispersive X-ray (EDX) and DLS also confirmed the presence of silver atoms.
Conclusion: Silver nanoparticles of Rhizophora mucronata bark revealed a well-defined structure and may be used in antimicrobial function in further researches.
Downloads
References
Narayanan KB, Sakthivel N. Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Adv Colloid Interface Sci 2011;169:2-59.
Priya K, Krishnakumari S. Phytochemical analysis of Achyranthes aspera and its activity on sesame oil induced lipid peroxidation. Anc Sci Life 2007;27:6-10.
Netala VR, Ghosh SB, Bobbu PL, Anitha D, Vijaya T. Triterpenoid saponins: A review on biosynthesis, applications and mechanism of their action. Int J Pharm Pharm Sci 2014;7:24-8.
Kathiresan K, Manivannan S, Nabeel MA, Dhivya B. Studies on silver nanoparticles synthesized by a marine fungus Penicillium fellutanum isolated from coastal mangrove sediment. Colloid Surf B 2009;71:133-7.
Leela A, Vivekanandan M. Tapping the unexploited plant resources for the synthesis of silver nanoparticles. Afr J Biotechnol 2008;7:3162-5.
Srikanth K, Vikram G, Rama Swamy N. Eco-friendly and green synthesis of silver nanoparticles using leaf extract of Strychnos potatorum Linn. F. and their bactericidal activities. 3 Biotech 2014;5:706-14.
Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci India 2003;85:162-70.
Thakkar KN, Mhatre SS, Parikh RY. Biological synthesis of metallic nanoparticles. Nanomedicine 2010;6:257-62.
Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM. A preliminary assessment of silver nanoparticle inhibition of monkey pox virus plaque formation. Nanoscale Res Lett 2008;3:129-33.
Panacek A, Kolar M, Vecerova R, Prucek R, Soukupová J, Krystof V, et al. Antifungal activity of silver nano particles against Candida spp. Biomaterials 2009;30:6333-40.
Keat CL, Aziz A, Eid AM, Elmarzugi NA. Biosynthesis of nanoparticles and silver nanoparticles. Bioresour Bioproc 2015;2:47.
Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci 2016;17:1534.
Patil RS, Kokate MR, Kolekar SS. Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 2012;91:234-8.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
