HYPOGLYCAEMIC EFFECT OF SYZYGIUM CARYOPHYLLATUM (L.) ALSTON ON ALLOXAN INDUCED DIABETIC ALBINO MICE
Abstract
Objective: The primary objective of this study was to determine the hypoglycaemic activity of S. caryophyllatum in alloxan induced diabetic mice.
Methods: Alloxan was administered as a single dose (60mg/kg, b. wt) to induce diabetes. Methanol extracts from S. caryophyllatum (250mg/kg body weight/day) was administered for 14 days to alloxan induced diabetic mice and the body weight changes and fasting blood sugar levels were monitored on 0, 7th and 14th day. Glycosylated haemoglobin levels also estimated.
 Result: The results suggest that the administration of S. caryophyllatum have hypoglycaemic activity in alloxan induced diabetic mice and their effect was equivalent to that of reference drug Glibenclamide. Conclusions: The results suggested that the methanol extract of S. caryophyllatum  possess anti-diabetic effect on Alloxan induced diabetic mice and it can be recommended for the prevention of diabetes mellitus.
Keywords: Alloxan monohydrate, Glibenclamide, Glycosylated haemoglobinDownloads
References
Whiting DR, Guariguata L, Weil C, Shaw J. IDF Diabetes Atlas: Global estimates of the prevalence of diabetes for 2011 and 2030. Diab Res Clin Pr. 2011; 94: 311-321.
Geetanjali K, Santosh S, Rakesh KK, Naik SN, Commonly consumed Indian plant food materials in the management of diabetes mellitus, Diabetes and Metabolic Syndrome: Clin Res Rev. 2010; 4 (1): 21- 40.
Shokeen P, Anand P, Murali YK, Tandon V. Antidiabetic activity of 50% ethanolic extract of Ricinus communis and its purified fractions. Food Chem Toxicol. 2008; 46(11): 3458-3466.
Sheela CG, Kumud K, Augusti KT. Antidiabetic effects of onion and garlic sulfoxide amino acids in rats. Planta Med. 1995; 61: 356-357.
Sitasawad SL, Shewade Y, Bhonde R, Role of bittergourd fruit juice in STZ-induced diabetic state in vivo and in vitro. J Ethnopharmacol. 2000; 73: 71-79.
Soni H, Nayak G, Patel SS, Mishra K, Singhai AK. Pharmacognostic studies of the leaves of Syzygium cumini Linn. Int J Res Pharm Biomed Sci. 2011; 2 (2): 507-509.
Nadkarni KM. In Indian Materia medica. Vol. I (Popular book depot Bombay), 1954. p. 516-518.
Shrotri DS, Kelkar M, Deshmukh VK, Aiman R. Investigations of the hypoglycemic properties of Vinca rosea, Cassia auriculata and Eugenia jambolana. Ind Medical Res. 1963; 51: 464-467.
Bansal R, Ahmad N, Kidwai JR. Effect of oral administration of Eugenia jambolana seeds and chlorpropamide on blood glucose level and pancreatic cathepsin B in rats. Indian J Biochem Biophys. 1981; 18: 377- 381.
Acherekar S, Kaklij GS Kelkar SM. Hypoglycemic activity of Eugenia jambolana and Ficus bengalensis: mechanism of action. In vivo. 1991; 5: 143-147.
Grover JK, Vats V, Rathi SS. Antihyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol. 2000; 73: 461-470.
Sharma S, Nasir A, Prabhu KM, Murthy PS, Dev G. Hypoglycaemic and hypolipidemic effect of ethanolic extract of seeds of Eugenia jambolana in alloxan-induced diabetic rabbits. J Ethnopharmacol. 2003; 85: 201-206.
IUCN. Syzygium benthamianum. In: IUCN 2011. IUCN Red List of Threatened Species. Version 2011.1. Available from: http://www.iucnredlist.org. [Last accessed on 2011].
Sasaki T, Matsuy S, Sanae A, Effect of acetic acid concentration on the colour reaction in the O-toluidine boric acid method for blood glucose determination. Ransho Kagajci., 1972; 1: 346-350.
Kynoch PA, Lehmann H. Rapid estimation of glycosylated haemoglobin for roteine purposes. Lancet. 1977; 2(8027):16-16.
Gurusamy K, Kokilavani R, Sowmya C, Arumugasamy K. Non enzymatic antioxidant properties of ethanolic extract of polyherbal formulation (PHF) against CCL4 induced rats. Res J Biotechnol. 2008; 3: 51-54.
Shrotri DS, Kelkar M, Deshmukh VK, Aiman R. Investigations of the hypoglycemic properties of Vinca rosea, Cassia auriculata and Eugenia jambolana. Ind Medical Res. 1963; 51: 464-467.
Eshrat HM. Hypoglycemic, hypolipidemic and antioxidant properties of combination of curcumin from curcuma longa Linn. and partially purified product from Abroma augusta, Linn. In streptozotocin induced diabetes. Indian J Clin Biochem. 2002; 17(2): 33-43.
Tiedge M, Lenzen S, Effects of glucose reffeding and glibenclamide treatment on glucokinase and GLUT2 gene expression in pancreatic B-cells and liver from rats. Biochem Journal. 1995; 308: 139-144.
Srivastava S, Lal VK, Pant KK, Polyherbal formulations based on Indian medicinal plants as antidiabetic phytotherapeutics. Phytopharmacol. 2012; 2(1): 1-15.
Aybar MJ, Riera ANS, Grau A, Sanchez SS. Hypoglycemic effect of the water extract of Small Antus sonchifolitus (yacon) leaves in normal and diabetic rats. J Ethnopharmacol. 2002; 74: 125-132.
Thai AC, Yeo PPB, Chan L, Wang KW, Tan BY, Jocobs E, Glycosylated haemoglobin and diabetic control. Singapore Med J. 1983; 24: 210-212.
Pari L, Amarnath Satheesh M, Antidiabetic activity of Boerhaavia diffusa L. effect on hepatic key enzymes in experimental diabetes. J Ethnopharmacol. 2004; 91(1): 109-13.
Jamshid M, Prakash R, Naik. Evaluation of hypoglycemic effect of Morus alba in an animal model. Indian J Pharmacol. 2008; 40: 15-18.
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