ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF ERUCA SATIVA (JARJEER) LEAVES EXTRACT ON CARCINOMA OF WOMEN’S BREAST
DOI:
https://doi.org/10.22159/ajpcr.2021.v14i2.39337Keywords:
Eruca sativa, Cell line of breast cancer, Apoptosis, DNA fragmentation, AntioxidantsAbstract
Objective: This work aims to investigate the influence of Eruca sativa leaves extract on the cell viability of the breast carcinoma cell line (MCF-7).
Methods: In vitro, breast cancer cell line (MCF-7) treated by E. sativa leaves extract for 48 h. The cell viability, proliferation, and apoptosis were assessed using colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric technique, and antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]) measurement.
Results: This study demonstrated that the incubation of MGF-7 cells with E. sativa for 48 h caused a significative reduction in cell viability and proliferation of MGF-7 cell line. In parallel, E. sativa treatment induces a significant increase in apoptosis of MGF-7 cells compared to control. Moreover, flow cytometry analysis demonstrated that the inhibition of MGF-7 cell proliferation existed at the G2 and M phase in the cell-division cycle. Finally, the intracellular antioxidant enzymes SOD and CAT activities were significantly increased in the administered cells compared with unadministered MCF-7 cells.
Conclusions: Taken together, E. sativa treatment reduces cell viability and proliferation concomitant with enhanced antioxidant enzymes expression and apoptosis of breast cancer cell line MGF-7. This may help in protection from breast cancer or preclinical recommendation.
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Ibrahim AS, Khaled HM, Mikhail NN, Baraka H, Kamel H. Cancer incidence in Egypt: Results of the national population-based cancer registry program. J Cancer Epidemiol 2014;2014:437971.
George VC, Kumar DR, Kumar ND, Rajkumar V, Suresh P, Kumar RA. Quantitative assessment of the relative antineoplastic potential of the n-butanol leaf extract of Annona muricata Linn. In normal and immortalized human cell lines. Asian Pac J Cancer Prev 2012;13:699-704.
Barlas NT, Irget ME, Tepecik M. Mineral content of the rocket plant (Eruca sativa). Afr J Biotechnol 2011;10:14080-2.
El-Wakeel MA, El-Desoki ER, Ahmed SE. Bioherbicidal activity of Eruca sativa fresh shoot aqueous extract for the management of two annual weeds associating Pisum sativum plants. Bull Natl Res Centre 2019;43:87.
El-Fadaly H, El-Kadi S, El-Moghazy M, Soliman A, El-Haysha M. Antioxidant activity studies on extracts of Eruca sativa Seed meal and oil, detoxification, the role of antioxidants in the resistant microbes. Hum J 2017;6:31-51.
Radziejewska-Kubzdela E, Olejnik A, Biega?ska-Marecik R. Effect of pretreatment on bioactive compounds in wild rocket juice. J Food Sci Technol 2019;56:5234-42.
Alqasoumi S. Carbon tetrachloride-induced hepatotoxicity: Protective effect of Rocket Eruca sativa L. in rats. Am J Chin Med 2010;38:75-88.
Oka M, Maeda S, Koga N, Kato K, Saito T. A modified colorimetric MTT assay adapted for primary cultured hepatocytes: Application to proliferation and cytotoxicity assays. Biosci Biotechnol Biochem 1992;56:1472-3.
Fabisiak JP, Borisenko GG, Kagan VE. The quantitative method of measuring phosphatidylserine externalization during apoptosis using electron paramagnetic resonance (epr) spectroscopy and annexin-conjugated iron. Methods Mol Biol 2014;1105:613-21.
Nishikimi M, Roa NA, Yogi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849-54.
Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
Michael HN, Shafik RE, Rasmy GE. Studies on the chemical constituents of the fresh leaf of Eruca sativa extract and its biological activity as an anticancer agent in vitro. J Med Plants Res 2011;5:1184-91.
Azarenko O, Jordan MA, Wilson L. Erucin, the major isothiocyanate in arugula (Eruca sativa), inhibits proliferation of MCF7 tumor cells by suppressing microtubule dynamics. PLoS One 2014;9:1-12.
Pawlik A, Skan Wojewódzka MS, Herman-Antosiewicz A. Sensitization of estrogen receptor-positive breast cancer cell lines to 4-hydroxytamoxifen by isothiocyanates present in cruciferous plants. Eur J Nutr 2016;55:1165-80.
Ang E, Pavlos N, Chim S, Feng HT, Scaife RM, Steer JH, et al. Paclitaxel inhibits osteoclast formation and bone resorption via influencing mitotic cell cycle arrest and RANKL-induced activation of NF-kappaB and ERK. J Cell Biochem 2012;113:946-55.
Alam MS, Kaur G, Jabbar Z, Javed K, Athar M. Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride-induced renal toxicity. Food Chem Toxicol 2007;45:910-20.
Hussein J, Salah A, Oraby F, Nour El-Deen A, El-Khayat Z. Antihepatotoxic effect of Eruca sativa extracts on alcohol-induced liver injury in rats. J Am Sci 2010;6:381-9.
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