To evaluate the analgesic activity of Ficus Racemosa leaf extract in albino mice using Eddy's hot plate method.
Abstract
Objective-To evaluate the analgesic activity of ficus racemosa leaf extract in albino mice and its comparison with standard drug using Eddy's hot plate method.
Method-Chloroform leaf extract was obtained using soxhlet apparatus. Isolated fraction of extract (FRE) was used for the experiments. Swiss albino mice of either sex, average weight 20-25gms were used for experiments. Animals were divided into 6 groups consisting of 6 animals each. In hot plate method, Basal reaction time was recorded. Each group received particular treatment and reaction time was recorded after half, one, two and four hours. Pentazocine was taken as standard drug.
Result-FRE at the dose of 50mg/kg did not shows any significant analgesic activity while at the dose of 100 and 200 mg/kg it showed significant analgesic activity as compare to control group. FRE at dose of 200mg/kg showed significant analgesic activity as compare to pentazocin. Pentazocin in combination with 50mg/kg dose of FRE produced significant analgesic activity when compared to control value or either treatment alone.
Conclusion-FRE endowed with central analgesic properties in dose dependent manner. And also enhances the analgesic effect of pentazocin. However further study is needed in order to understand the precise mechanism.
Keywords- ficus racemosa, analgesics, pentazocin, albino mice, Eddy's hot plate.
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Kalgutkar AS, Marnett AB. Crews BC, Remmel RP, Marnett. LJ. Ester and amide derivatives of the nonsteroidalantiinflammatory drug, indomethacin, as selectivecyclooxygenase-2 inhibitors. J Med Chem. 2000 Jul27;43(15):2860-70.
Anonymous. The Wealth of India. Council of Scientific and Industrial Research, New Delhi, India, 1952, pp. 35-36.
Mandal SC, Tapan K, Maity J, Das M, Pal M, Saha BP, Hepatoprotective activity of Ficus racemosa leaf extract on liver damage caused by carbon tetrachloride in rats. Phytother. Res., 13: (5) 1999, 430-432.
Khan N, Sultana S, Chemomodulatory effect of Ficus racemosa extract against chemically induced renal carcinogenesis and oxidative damage response in Wistar rats. Life Sci., 29, 2005.1194-1210.
Rao BR, Murugesan T, Sinha S, Saha BP, Pal M, Mandal SC. Glucose lowering efficacy of Ficus racemosa bark extract in normal and alloxan diabetic rats. Phytother. Res., 16, 2002, 590-592.
Mandal SC, Saha BP and Pal M, studies on bacterial activity of Ficus racemosa leaf extract, phytother Res, 2000, 14(4)278-280.
Rao BR, Anipama K, Swaroop A, Murugesan T, Pal M, Mandal SC, Evaluation of anti-pyretic potential of Ficus racemosa bark. Phytomedicine, 9, 2002, 731-733.
Rao BR, Murugesan T, Pal M, Saha BP, Mandal SC. Antitussive potential of methanol extract of stem bark of Ficus racemosa Linn. Phytother. Res., 17,2003, 1117- 1118.
Ratnasooriya WD, Jayakody JR, Nadarajah T. Antidiuretic activity of aqueous bark extract of Sri Lankan Ficus racemosa in rats. Acta Biol Hung. 54(3-4):2003, 357-63.
Kokate C.K., Textbook of practical pharmacognosy, 4th ed. 2003, p 143, Vallabh Prakashan, New Delhi.
Ghani A, 2003. Medicinal Plants of Bangladesh. The Asiatic Society of Bangladesh. Dhaka, Bangladesh, 500-504.
Organization for Economic Cooperation and Development. OECD Guidelines for the Testing of Chemicals. OECD Guideline 420: Acute Oral Toxicity: Up-and-Down Procedure, Approved: December 2001.
Baby Joseph, S.Justin Raj, Phytopharmacological properties of Ficus Racemosa Linn- an overview. International Journal of Pharmaceutical Sciences Review and Research Volume 3, Issue 2, July – August 2010; Article 025.
Sabina EP, Chandel S, Rasool MK, 2009. Evaluation of analgesic, antipyretic and ulcerogenic effect of Withaferin A. International Journal of Integrative Biology, 6 (2): 52-56.
Ibironke GF, Ajiboye KI, 2007. Studies on the anti-inflammatory and analgesic properties of Chenopodium ambrosioides leaf extract in rats. International Journal of Pharmacology, 3: 111-115.
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