FOURIER TRANSFORM INFRARED ANALYSIS OF ULVA LACTUCA AND GRACILARIA CORTICATA AND THEIR EFFECT ON ANTIBACTERIAL ACTIVITY
Abstract
ÂIn the present work, we used two seaweeds Ulva lactuca and Gracilaria corticata, which were collected from Hare Island in the Gulf of Mannar of
Tuticorin coast. Ethanol was taken as the solvent for extraction. The crude extract was purified using column chromatography. Antibacterial activity
of crude and column purified fractions were tested against Klebsiella, Aeromonas, Staphylococcus, Escherichia and Pseudomonas using well-diffusion
method. Maximum zone of inhibition (9 mm) was found in the crude extract of G. corticata against Pseudomonas sp. Minimum zone of inhibition (4
mm) was found in U. lactuca fraction1 against Escherichia coli. Highest antibacterial activity was obtained in red seaweed, whereas, green seaweed
showed less antibacterial activity. From this study, we can conclude that red seaweeds have more active than green seaweeds. The seaweed powder
was analyzed in Fourier transform infrared spectrometer. It was observed that both seaweed contained phenol and alcohol compounds, which were
responsible for the antibacterial activity.
Keywords: Antibacterial activity, Fourier transform infrared, Seaweeds, Ulva lactuca, Gracilaria corticata.
Â
Downloads
References
Oh, K.B., Lee, J.H., chang, S.C., Shin, J., Shin, H.J., Kim, H.K.. Antimicrobial activity of the bromophenols from red alga Odonthaliacorymbifera and some synthetic derivatives. Bioorganic and Med ChemLett. 2008. 18:104-108.
Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutat Res 2005; 579: 200-213.
Badea V, Balaban DP, Rapeanu G, Maria C, Badea CF. The antibacterial activity evaluation of Cystoseirabarbata biomass and some agents upon bacteria from oropharyngeal cavity. Rom Soc Bio Sci 2009; 14: 4851-4857.
Rajasekar, T., P. Priyadharshini, B.Deivasigamani ,S.Kumaran, G.George Edward, M.Sakthivel and Balamurugan S.2013. Isolation of bioactive compound from marine seaweeds against fish pathogenic bacteria Vibrio alginolyticus (VA09) and characterisation by FTIR. Journal of Coastal Life medicine 1(1): 6-13.
Vijayabaskar P, Shiyamala V. Antibacterial activities of brown marine algae (Sargassumwightii and Turbinariaornata) from the Gulf of Mannar Biosphere Reserve. AdvBiol Res 2011; 5: 99- 102.
Siddhanta AK, Mody KH, Ramavat BK, Chauhan VD, Garg HS, Goel AK, et al. Bioactivity of marine organisms: Part VIII - screening of some marine flora of western coast of India. Indian J ExpBiol 1997; 35: 638-643.
Paul G, Yusuf S, Sharma S. Unmasking of the Brugada syndrome phenotype during the acute phase of amiodarone infusion. Circulation 2006; 114(11): e489-e491.
Afifah SN, Darah I, Fariza SS, Nordin MK, Aili ZN. Antimicrobial activity of various extracts of a tropical Chlorophyta macroalgae, Halimeda discoidea. J ApplSci 2010: 10: 3007-3013.
Oulahal N, Adt I, Mariani C, Carnet-PantiezA, Notz E and Degraeve P, Examination of
wooden shelves used in the ripening of araw milksmear cheese by FTIRspectroscopy. Food Control, 20:658–663,(2009)
Ravikumar S, Ramanathan G, Subhakaran M, Jacob Inbaneson S. Antimicrobial compounds from marine halophytes for silkworm disease treatment. Int J Med Sci 2009; 1(5): 184-191.
Kolanjinathan K, Ganesh P, Govindarajan M. Antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens. Eur Rev Med PharmacolSci 2009; 13: 173-177.
Kim, SK., and Wijesekara, I. Development and biological activities of marine derived bioactive peptides: A review. J Funct Foods 2010; 2: 1-9.
Kristin Lammers., Georgia Arbuckle- Keil., and John Dighton.. FT-IR study of the changes in carbohydrate chemistry of three New Jersey pine barrens leaf litters during simulated control burning. Soil Biology & Biochemistry 2009; 41: 340 347.
Cowan MM. Plants products as antimicrobial agents, Clinical Microbiology Review 1999; 12: 564-582.
Barros L, Calhelha RC, Vaz JA Sablel, Ferreira CFR. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. Eur Food Res Technol 2007; 225: 151-156.
Kostic DA, Velickovic JM, Mitic SS, Mitic MN, Randelovic SS.Phenolic content, and antioxidant and antimicrobial activities ofCrataegusoxyacantha L (Rosaceae) fruit extract from SoutheastSerbia. Trop J Pharma Res 2012; 11(1): 117-124.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.