IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF CALOTROPIS PROCERA, EICHHORNIA CRASSEPIE AND DATURA INNOXIA LEAVES
Abstract
Medicinal plants are used for the formation of drugs and these plants are used traditionally to cure various diseases. These medicinal plants contain some phytochemical active compounds such as flavonoids, alkaloids, tannins, and phenols etc., which show antimicrobial activity against the pathogens. The present study investigated the antimicrobial activity of ethanol, methanol and aqueous extract of Calotropis procera, Eichhornia crassipes, and Datura innoxia plants, as determined on four pathogenic bacteria's i.e. Escherichia coli (MTCC-40), Staphylococcus epidermis (MTCC-10623), Pseudomonas aeruginosa (MTCC- 424), and Bacillus subtilius (MTCC- 736) by using well diffusion method. DMSO (Dimethyl Sulphoxide) was used as solvent for plant extracts. Ampicillin and tetracycline was set as a standard and also used to compare the antimicrobial activity of plants on pathogens. Phytochemical analysis was also done for the presence of bioactive compounds. The results showed that the plants extracts of Calotropis procera, Eichhornia crassipes, and Datura innoxia have potent antimicrobial activity against pathogens.
Keywords: Phytochemical, antimicrobial, Calotropis procera, Eichhornia crassipes, Datura innoxia, pathogens, Ampicillin, tetracycline.
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Kawo AH, Mustapha A, Abdullahi BA, Rogo LD, Gaiya ZA, Kumurya AS. Phytochemical properties and antibacterial activities of the leaf and latex extracts of Calotropis procera (AIT.F.) AIT.F. Bajopas 2009; 2(1): 34 -40.
Pal SK, Shukla Y. Herbal Medicine: Current Status and the Future. Asian Pac J Cancer Prev 2003 ; 4(4):281-8.
Cowan MM. Plant products as antimicrobial agents. Clin Micro Rev 1999;12(4): 564–582.
Kareem SO, Akpan I, Osho MB. Calotropis procera (Sodom apple) – A potential material for enzyme purification. J Bios Tech 2003 ;87(1) :133-135.
Umar, Mohammed BA. Effect of Water Hyacinth (Eichhornia Crassipes (Mart) Solms Leaf Extract on the Juvenile Mortality of Meloidogyne Incognita. J Agri Vete Sci 2013; 4(2) :46-48.
Vasu K, Goud JV, Suryam MA, Charya MAS. Biomolecular and phytochemical analyses of three aquatic angiosperms. Afri J Micro Res 2009; 3(8): 418-421.
Lattanzio V, Lattanzio VMT , Cardinali A. Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochemistry: Adv Res 2006; 23-67.
Cheesbrough M. District Laboratory practice in Tropical countriesâ€, Cambridge University Press London 2002; 2: 137-140.
Thamaraiselvi, Lalitha P, Jayanthi P. Preliminary studies on phytochemicals and antimicrobial activity of solvent extracts of Eichhornia crassipes (Mart.) Solms. Asia J Plan Sci Res 2012; 2 (2):115-122.
Satish S, Mohana DC, Raghavendra MP , Raveesha KA. Antifungal activity of some plant extracts against important seed borne pathogens of Aspergillus sp.. J Agri Tech 2007; 3(1): 109-119.
Kaushik P, Goyal P. "In vitro evaluation of Datura innoxia (thorn-apple) for potential antibacterial activity." Ind J Micro 2008; 48 (3):353-357.
Vadlapudi V. In vitro antimicrobial activity of methanolic extract of selected indian medicinal plants. Pharmacophore 2010; 1 (3): 214-219.
Ahmad S, Garg M, Tamboli ET, Abdin MZ, Ansari SH. In vitro production of alkaloids: Factors, approaches, challenges and prospects. Pharmaco Rev 2013; 7(13), 27-33.
Johnson DB, Shringi BN, Patidar DK., Chalichem NSS, Javvadi AK. Screening of antimicrobial activity of alcoholic & aqueous extract of some indigenous plants. Indo-Glo J Phar Sci 2011;1(2):186-193.
Baral B, Vaidya GS, Bhattarai N. Bioactivity and biochemical analysis of water hyacinth (Eichhornia crassipes). Botanica Orientalis: J Pla Sci 2012;8: 33-39.
Mako GA, Memon AH, Mughal AR, Pirzado AJ, Bhatti SA. Antibacterial effects of leaves and root extract of Calotropis procera Linn. Paki J Agri, Agri Engi Vet Sci 2012;28 (2):141-149.
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