THE PRESENCE OF A LITTERMATE DURING MEASUREMENT OF BLOOD PRESSURE REDUCES THE ACCLIMATIZATION TIME IN CONSCIOUS NAÃVE RATS.
Abstract
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Objective: Familiarizing an animal to the procedure is necessary for accurate measurement of blood pressure (BP). However, this requirement
considerably prolongs the procedure, which in some instances could add to spurious results. There is, therefore, a need to establish ways that will
help reduce stress and minimize the duration required for BP measurements in conscious animals. This study therefore examined the effect of the
presence of a littermate on the duration to achieve the first acceptable blood BP measurement using tail-cuff plethysmography in naïve conscious rats.
Methods: BP was measured in 12-weeks old, in-house naïve male Sprague-Dawley rats, either without prior familiarization and without a littermate
or after familiarization for 15 minutes, but without a littermate or after familiarization and with a naïve male littermate or without familiarization but
with a naïve male littermate or without familiarization but with a naïve female littermate. Time to the first accepted measurement was recorded. Data
were analyzed using one-way ANOVA and post-hoc analysis.
Results: The duration to the first acceptable BP measurement in familiarized and naïve rats was significantly shorter in the presence of a male
littermate (p<0.001), but not with a female littermate when compared with that in naïve rats without acclimatization or the presence of a littermate.
Conclusion: It appears that the presence of a male littermate when measuring BP in naïve male rats significantly decreases the duration required for
the measurement of BP. This might improve the efficiency of the procedure and even produce better data.
Keywords: Familiarization, Littermate, Tail-cuff rat, Blood pressure.
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References
Gross V, Luft FC. Exercising restraint in measuring blood pressure in conscious mice. Hypertension 2003;41:879-81.
Khorshid O, Abdel-Ghaffar E, Mishriki A, Galal A, Hareedy A. Possible cardiovascular protective effect of some PPAR activators in experimentally-induced hypertensive model in rats. Asian J Pharm Clin Res 2012;5(3):67-72.3. Devi J, Pamba P. Antihypertensive activity of bamboo shoot: A review. Asian J Pharm Clin Res 2015;8(1):46-7.
Reddy AK, Taffet GE, Madala S, Michael LH, Entman ML, Hartley CJ. Noninvasive blood pressure measurement in mice using pulsed Doppler ultrasound. Ultrasound Med Biol 2003;29(3):379-85.
Feng M, Whitesall S, Zhang Y, Beibel M, D’Alecy L, DiPetrillo K. Validation of volume-pressure recording tail-cuff blood pressure measurements. Am J Hypertens 2008;21(12):1288-91.
Buñag RD, Butterfield J. Tail-cuff blood pressure measurement without external preheating in awake rats. Hypertension 1982;4(6):898-903.
Williams TD, Chambers JB, Henderson RP, Rashotte ME, Overton JM. Cardiovascular responses to caloric restriction and thermoneutrality in C57BL/6J mice. Am J Physiol Regul Integr Comp Physiol 2002;282(5):R1459-67.
Kubota Y, Umegaki K, Kagota S, Tanaka N, Nakamura K, Kunitomo M, et al. Evaluation of blood pressure measured by tail-cuff methods (without heating) in spontaneously hypertensive rats. Biol Pharm Bull 2006;29(8):1756-8.
Irvine RJ, White J, Chan R. The influence of restraint on blood pressure in the rat. J Pharmacol Toxicol Methods 1997;38(3):157-62.
Hennessy MB, Kaiser S, Sachser N. Social buffering of the stress response: Diversity, mechanisms, and functions. Front Neuroendocrinol 2009;30(4):470-82.
Stamp J, Herbert J. Corticosterone modulates autonomic responses and adaptation of central immediate-early gene expression to repeated restraint stress. Neuroscience 2001;107(3):465-79.
Kvetnansky R, McCarty R, Thoa NB, Lake CR, Kopin IJ. Sympatho-adrenal responses of spontaneously hypertensive rats to immobilization stress. Am J Physiol 1979;236(3):H457-62.
Chiueh CC, Kopin IJ. Hyperresponsivitiy of spontaneously hypertensive rat to indirect measurement of blood pressure. Am J Physiol 1978;234(6):H690-5.
Kurtz TW, Griffin KA, Bidani AK, Davisson RL, Hall JE. Recommendations for blood pressure measurement in animals: Summary of an AHA Scientific Statement from the Council on High Blood Pressure Research, Professional and Public Education Subcommittee. Arterioscler Thromb Vasc Biol 2005;25:478-9.
Kodama Y, Kiyokawa Y, Takeuchi Y, Mori Y. Twelve hours is sufficient for social buffering of conditioned hyperthermia. Physiol Behav 2011;102(2):188-92.
da Costa AP, Leigh AE, Man MS, Kendrick KM. Face pictures reduce behavioural, autonomic, endocrine and neural indices of stress and fear in sheep. Proc Biol Sci 2004;271(1552):2077-84.
Wilson JH. Prolactin in rats is attenuated by conspecific touch in a novel environment. Cogn Affect Behav Neurosci 2001;1:199-205.
Kiyokawa Y, Wakabayashi Y, Takeuchi Y, Mori Y. The neural pathway underlying social buffering of conditioned fear responses in male rats. Eur J Neurosci 2012;36(10):3429-37.
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