QUANTITATIVE ANALYSIS OF TOTAL PHENOLS AND FLAVONOIDS IN IN VIVO AND IN VITRO SAMPLES OF OROXYLUM INDICUM (L.) VENT.
Abstract
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Objective: This paper presents flavonoids and phenol content of methanolic extract of in vivo and in vitro sample of Oroxylum indicum (L.) Vent. an
important medicinal plant. Selected plant contains number of phytochemical in its various parts such as stem bark, root bark, and leaves. This study
involves quantitative determine of phenolics and flavonoids content using Folin–Ciocalteau and quercetin as standard.
Methods: For investigation of in vitro culture, callus was prepared using apical bud and axillary bud as an explants. Explants were cultured on
MS medium supplemented with different concentration of 6-benzylaminopurine (BAP), kinetin (KN) and indole-3-acetic acid (IAA) for shoot
multiplication and BAP, KN and 2,4- dichlorophenoxyacetic acid 2,4-D for callus induction.
Results: BAP combination with IAA was best for shoot multiplication, BAP with 2,4-D were used for callus proliferation. The flavonoid and phenolics
contents in the in vivo and in vitro plant parts were found to be present in methanolic extract. The results show high phenolic and flavonoids content
from in vitro root of the plant parts.
Conclusion: There is great variation in secondary metabolites accumulation in different plant parts in both in vitro and in vivo developed condition.
Keywords: Flavonoid, Phenol, Oroxylum indicum.
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