ANTIPROLIFERATIVE AND APOPTOTIC ACTIVITY OF SULFATED POLYSACCHARIDE ISOLATED FROM HYPNEA VALENTIAE RED SEAWEED IN HUMAN SKIN MALIGNANT MELANOMA CELLS

NEGHA RAJENDRAN; RAMYA RAVICHANDRAN; VEERABHUVANESHWARI VEERICHETTY

doi:10.22159/ajpcr.2021.v14i8.42048

Authors

NEGHA RAJENDRAN Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, Tamil Nadu, India.
RAMYA RAVICHANDRAN Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, Tamil Nadu, India.
VEERABHUVANESHWARI VEERICHETTY Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i8.42048

Keywords:

Anti-proliferative, Cell viability, Cytotoxicity, Human skin malignant melanoma cells, L6 (Rat skeletal muscle cells)

Abstract

Objective: Malignant melanoma is a highly metastatic cutaneous cancer. Deregulated apoptosis has been identified as a major cause of cancer drug resistance. The objective of the study is to evaluate antiproliferative activity of Hypnea Valentiae extract in human skin malignant melanoma (SK-MEL) cells.

Methods: In this study, sulfated polysaccharide fraction was precipitated from aqueous extract obtained from H. valentiae. MTT assay was used to determine the cell viability of the crude sulfated polysaccharide against SK-MEL cells and normal L6 cell line (Rat skeletal muscle). Acridine orange (AO) and Ethidium bromide (EB) staining method was applied to study induction of apoptosis in SK-MEL cells.

Results: Dose-dependent reduction in cell viability was observed with an IC50 of 30 μg/ml in SK-MEL cancer cells. The sulfated polysaccharide treated SK-MEL cells followed by AO, EB staining, showed typical early apoptotic, and late apoptotic morphological changes.

Conclusion: The isolated crude sulfated polysaccharide from H. valentiae produced potent growth inhibition and induction of apoptosis in SK-MEL cells but caused no cytotoxicity in normal L6 skeletal muscle cells.

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