RELATIONSHIP OF SEMINAL FRUCTOSE AND SERUM PROLACTIN LEVELS IN INFERTILE MEN

JUHI KATARIA; GURPREET KAUR GILL; LOUIS COJANDARAJ

doi:10.22159/ajpcr.2021.v14i11.42909

Authors

JUHI KATARIA Department of Medical Laboratory Sciences, Khalsa College of Pharmacy and Technology, Amritsar, Punjab, India.
GURPREET KAUR GILL Department of Medical Laboratory Sciences, Khalsa College of Pharmacy and Technology, Amritsar, Punjab, India.
LOUIS COJANDARAJ Department of Medical Laboratory Sciences, Lovely School of Allied Medical Sciences, Lovely Professional University, Phagwara, Punjab, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i11.42909

Keywords:

Infertility, Motility, Semen analysis, Furtose, Erythrocyte sedimentation rate

Abstract

Objective: Male infertility refers to a male’s inability to cause pregnancy in a fertile female. Infertility is a widespread problem for about one in five infertile couples. Male infertility is usually caused by a problem that affects either sperm production or sperm transport. The aim of the study was to evaluate prolactin levels in the serum and comparing the levels of fructose in the semen of infertile males and normal healthy controls of reproductive age of 20–45 years respectively.

Methods: Hemoglobin (Hb) level in the blood of both groups was calculated by Sahli’s method using Sahli’s Hemoglobinometer. The Erythrocyte Sedimentation Rate (ESR) was determined using the modified Westergren’s method and total leukocyte count (TLC) in the blood was determined by microdilution method. Semen analysis was performed by the method specified in the World Health Organization Laboratory Manual. Morphology and motility of sperm and their counts were determined by microscopy. Fructose drop test in semen was estimated colorimetrically by the Semen Fructose Estimation Kit and determination of serum prolactin involved the use of Electrochemiluminescence immunoassay using Cobas e 411 analyzer.

Results: Hb in infertile males was less than healthy controls. However, ESR in infertile men was considerably high and TLC, serum prolactin levels did not show much variation in infertile men. Seminal analysis of infertile men revealed high fructose levels and low sperm count with decreased motility.

Conclusion: The concentration of seminal fructose in control group is lower than infertile males. High fructose levels in infertile men and less sperm count indicate oligozoospermia.

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References

Kumar TA. In vitro fertilization in India. Curr Sci 2004;86:254-56.

Calverton M. USA: ORC Macro and the World Health Organization. World Health Organization. Infecundity, Infertility, and Childlessness in Developing Countries. Kerala: DHS Comparative Reports; 2004.

Kamel RM. Management of the infertile couple: An evidence-based protocol. Reprod Biol Endocrinol 2010;8:1-7.

Zegers-Hochschild F, Adamson GD, de Mouzon J, Ishihara O, Mansour R, Nygren K, et al. The international committee for monitoring assisted reproductive technology (ICMART) and the World Health Organization (WHO) revised glossary on ART terminology. Hum Reprod 2009;24:2683-7.

Schoenfeld CY, Amelar RD, Dubin L, Numeroff M. Prolactin, fructose, and zinc levels found in human seminal plasma. Fertil Steril 1979;32:206-8.

Gonzales GF. Function of seminal vesicles and their role on male fertility. Asian J Androl 2001;3:251-8.

Ndovi TT, Choi L, Caffo B, Parsons T, Baker S, Zhao M, et al. Quantitative assessment of seminal vesicle and prostate drug concentrations by use of a noninvasive method. Clin Pharmacol Ther 2006;80:146-58.

Raj V, Vijayan AN, Joseph K. Naked eye detection of infertility using fructose blue-a novel gold nanoparticle based fructose sensor. Biosens Bioelectron 2014;54:171-4.

Buckett WM, Lewis-Jones DI. Fructose concentrations in seminal plasma from men with nonobstructive azoospermia. Arch Androl 2002;48:23-7.

Ahmed ND, Ahmed HS. The relationships between serum prolactin level and semen analysis parameters among patients attended the reproductive health center, Khartoum 2016-2017. Sudan Med Monit 2017;12:41-4.

Luboshitzky R, Rosen E, Trestian S, Spitz IM. Hyperprolactinaemia and hypogonadism in men: Response to exogenous gonadotrophins. Clin Endocrinol News 1979;11:217-23.

Okada H, Iwamoto T, Fujioka H, Shirakawa T, Tatsumi N, Kanzaki M, et al. Hyperprolactinaemia among infertile patients and its effect on sperm functions. Andrologia 1996;28:197-202.

Berman L. The determination of hemoglobin by the acid hematin method. Arch Intern Med 1919;24:553-6.

Zhao TX, Lockner D. Electrical impedance and erythrocyte sedimentation rate (ESR) of blood. Biochim Biophys Acta Biomembr 1993;1153:243-8.

Blumenreich MS. The white blood cell and differential count. In: Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd ed. Boston: Butterworths; 1990. p. 153-77.

Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO laboratory manual for the examination and processing of human semen. Asian J Androl 2010;12:47-58.

Sánchez-Carbayo M, Mauri M, Alfayate R, Miralles C, Soria F. Analytical and clinical evaluation of TSH and thyroid hormones by electrochemiluminescent immunoassays. Clin Biochem 1999;32:395-403.

Nourshargh S, Alon R. Leukocyte migration into inflamed tissues. Immunity 2014;41:694-07.

Lu JC, Chen F, Xu HR, Huang YF, Lu NQ. Standardization and quality control for determination of fructose in seminal plasma. J Androl 2007;28:207-13.

Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: A review of literature. J Hum Reprod 2015;8:191-6.

Orakwe JC, Chukwuezi FO, Ebuh GU. True corrected seminal fructose in male infertility in Nigerians a preliminary study. Niger J Clin Pract 2010;13:84-6.

Tüttelmann F, Ruckert C, Röpke A. Disorders of spermatogenesis. Med Genet 2018;30:12-20.

Hermanns U, Hafez ES. Prolactin and male reproduction. Arch Androl 1981;6:95-125.