ANTIBIOTIC RESISTANCE PATTERN OF PSEUDOMONAS AERUGINOSA ISOLATED FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL, PUDUCHERRY

KANTHAKUMAR A; JAYAVARTHINNI M

doi:10.22159/ajpcr.2022.v15i2.43839

Authors

KANTHAKUMAR A Department of Microbiology, Indira Gandhi Medical College and Research Institute, Puducherry, India. https://orcid.org/0000-0002-2766-6422
JAYAVARTHINNI M Department of Microbiology, Indira Gandhi Medical College and Research Institute, Puducherry, India. https://orcid.org/0000-0002-2766-6422

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i2.43839

Keywords:

Multidrug resistance, Pseudomonas aeruginosa, Antimicrobial susceptibility pattern

Abstract

Objective: One of the most common bacteria known to cause nosocomial infection and found to be multidrug-resistant is Pseudomonas aeruginosa. The objective of the study was to know the prevalence of the P. aeruginosa isolates with varied clinical conditions and specimens and to assess the antimicrobial susceptibility patterns of P. aeruginosa as well as its magnitude of multidrug resistance (MDR).

Methods: A total of 229 biochemically tested and confirmed isolates of P. aeruginosa from various clinical samples were studied. Antibiotic susceptibility testing was determined by Kirby-Bauer disc diffusion method.

Results: Out of the 229 isolates of P. aeruginosa, majority (60.70%) were from pus sample. Resistance to amikacin and tobramycin was 23.6% and 20.1%, ciprofloxacin was 33.2%. Resistance to ceftazidime, cefoperazone and cefepime were 21.8%, 45.9%, and 25.7%. Imipenem and meropenem showed 26.2% and 20.5% resistance, respectively. Resistance to piperacillin was 18.3% while piperacillin-tazobactam was only 13.5%. The MDR was observed in 33.7% of the isolates.

Conclusion: There is increased resistance to cephalosporins as compared to aminoglycosides, carbapenems and beta lactamase inhibitor. To restrict the inappropriate use of antimicrobial agents, the development of MDR, needs to be continuously monitored and documented.

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References

Mohanasoundaram KM. The antibiotic resistance pattern in the clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital; 2008- 2010 (A 3 year study). J Clin Diagn Res 2011;5:491-4.

Kaur A, Singh S, Gill AK, Kaur N. Prevalence and antimicrobial susceptibility pattern of Pseudomonas aeruginosa isolated from various clinical samples in tertiary care Hospital, Bathinda. Indian J Basic Appl Med Res 2016;5:777-84.

Choudhary V, Pal N, Hooja S. Prevalence and antibiotic resistance pattern of Metallo-β-lactamase-producing Pseudomonas aeruginosa isolates from clinical specimens in a tertiary care hospital. J Mahatma Gandhi Inst Med Sci 2019;24:19-22.

Horcajada JP, Montero M, Oliver A, Sorlí L, Luque S, Gómez-Zorrilla S, et al. Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections. Clin Microbiol Rev 2019;32:e19-31.

Collee JG, Miles RS, Watt B. Tests for identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14th ed. Singapore: Churchill Livingstone; 2006. p. 131-49.

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty Seventh Informational Supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2017.

Chander A, Raza MS. Antimicrobial susceptibility patterns of Pseudomonas aeruginosa clinical isolates at a tertiary care hospital in Kathmandu, Nepal. Asian J Pharm Clin Res 2013;6:235-8.

Dash M, Padhi S, Narasimham MV, Pattnaik S. Antimicrobial resistance pattern of Pseudomonas aeruginosa isolated from various clinical samples in a tertiary care hospital, South Odisha, India. Saudi J Health Sci 2014;3:15-9.

Yadav S, Sujatha R, Deepak S. Emergence of multidrug resistant Pseudomonas aeruginosa in a tertiary care center. Int J Health Sci Res 2018;8:52-5.

Hoque MM, Ahmad M, Khisa S, Uddin MN, Jesmine R. Antibiotic resistance pattern in Pseudomonas aeruginosa isolated from different clinical specimens. J Armed Forces Med Coll Bangladesh 2015;11:45-9.

Tiwari N, Rajdev S, Mullan S. Resistance trends among Pseudomonas aeruginosa isolates in a tertiary care centre in South Gujarat. Adv Microbiol 2017;7:188-94.

Saroj G, Suhani, Manasa S, Jyoti. Isolation of Pseudomonas aeruginosa from various clinical isolates and it antimicrobial resistance pattern in a tertiary care hospital. Int J Curr Microbiol Appl Sci 2016;5:247-53.

Kumari M, Khurana S, Bhardwaj N, Malhotra R, Mathur P. Pathogen burden and associated antibiogram of Pseudomonas spp in a tertiary care hospital of India. Indian J Med Res 2019;149:295.

Rustini R, Jamsari J, Marlina M, Zubir N, Yuliandra Y. Antibacterial resistance pattern of Pseudomonas aeruginosa isolated from clinical samples at a general hospital in Padang, West Sumatra, Indonesia. Asian J Pharm Clin Res 2017;10:158-60.

Pramodhini S, Umadevi S, Seetha KS. Prevalence of Antimicrobial resistance in clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Puducherry, India. Int J Curr Microbiol Appl Sci 2015;4:718-26.