THE STUDY OF ANTIOXIDANTS TO EVALUATE THE HEALING EFFECTS OF PUNICA GRANATUM PEEL ON ACETIC ACID-INDUCED COLITIS IN CHARLES-FOSTER ALBINO RATS

ARPIT VERMA; ANAND BHADKARIYA; SUPRIYA SHARMA; NIKITA TOSHI

doi:10.22159/ajpcr.2022.v15i2.43895

Authors

ARPIT VERMA Department of Pharmacology, Government Medical College, Datia, Madhya Pradesh, India.
ANAND BHADKARIYA Department of Pathology, Government Medical College, Datia, Madhya Pradesh, India.
SUPRIYA SHARMA Department of Pharmacology, Netaji Subhash Chandra Bose Medical College, Jabalpur, Madhya Pradesh, India.
NIKITA TOSHI Department of Psychiatry, Government Medical College, Datia, Madhya Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i2.43895

Keywords:

Ulcerative colitis, Punica granatum peel extract, Acetic acid, Sulfasalazine, Antioxidants

Abstract

Objective: The study aims to analyze the effect of 50% ethanolic extract of the dried Punica granatum peel (PGE) on the healing of acetic- acid-induced colitis in rats.

Methods: Colitis was induced in rats using 50% acetic acid and then PGE extract was administered by oral route daily for 14 days to those rats. Optimal healing was observed by the administration of a 100 mg/kg dose of PGE extract. Effectiveness of the above-mentioned dosage of PGE, biochemical parameters namely- antioxidants-superoxide dismutase and reduced glutathione were studied on 18 h fasting rats on the 15th day of the experiment.

Results: The results were suggestive of the healing properties of PGE extract by reduction of the inflammation and mucosal damage in the colon of those rats. The effect was established by the levels of antioxidants that indicate healing of the mucosal damage. The safety of extract was established by the effective administration of 10 times the therapeutic dose, that is, 1000 mg/kg dosage of the PGE extract with no noticeable adverse effects or side effects related to autonomic nervous system or central nervous system.

Conclusion: PGE extract was found to be effective in healing mucosal damage due to colitis by controlling the infection and reducing the inflammation.

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References

Kathleen A, Jurenka JS. Inflammatory bowel disease Part I: Ulcerative colitis pathophysiology and conventional and alternative treatment options. Altern Med Rev 2003;8:247-83.

Benson JM, Shepherd DM. Aryl hydrocarbon receptor activation by TCDD reduces inflammation associated with Crohn’s disease. Toxicol Sci 2010;120:68-78.

Molodecky NA, Kaplan GG. Environmental risk factors for inflammatory bowel disease. Gastroenterol Hepatol 2010;6:339-46.

Khor B, Gardet A, Xavier RJ. Genetics and pathogenesis of inflammatory bowel disease. Nature 2011;474:307-17.

Akhlaghi M, Band B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J Mol Cell Cardiol 2009;46:309-17.

Gautam MK, Goel S, Ghatule RR, Singh A, Nath G, Goel RK, et al. Curative effect of Terminalia chebula extract on acetic acid-induced experimental colitis: Role of antioxidants, free radicals and acute inflammatory marker. Inflammopharmacol 2012;21:377-83.

Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 2008;3:163-75.

Tahan G, Aytac E, Aytekin H, Gunduz F, Dogusoy G, Aydin S, et al. Vitamin E has a dual effect of anti-inflammatory and ulcerative colitis in rats. Can J Surg 2011;54:333-8.

Keshavarzian A, Banan A, Farhadi A, Komanduri S, Mutlu E, Zhang Y, et al. Increases in free radicals and cytoskeletal protein oxidation and nitration in the colon of patients with inflammatory bowel disease. Gut 2003;52:720-8.

Lansky EP, Newman RA. Punicagranatum and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol 2007;109:177-206.

Pirbalouti AG, Koohpayeh A, Karimi I. The wound healing activity of flower extracts of Punicagranatum and Achilleakellalensis in wistar rats. Actapolonia Pharm Drug Res 2010;67:107-10.