PHYTOCHEMICAL SCREENING WITH LC-HRMS PROFILING AND IN VITRO BIOLOGICAL ACTIVITIES OF ARGYREIA CUNEATA (L.) AND ARGYREIA SETOSA (L.).

Sandip Kale; Pranoti Kirdat; Suresh Kale; Padma Dandge

doi:10.22159/ajpcr.2022.v15i10.45502

Authors

Sandip Kale Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, India.
Pranoti Kirdat Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, India.
Suresh Kale Department of Botany, Sathaye College, Vile Parle, Mumbai, Maharashtra, India.
Padma Dandge Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i10.45502

Keywords:

Argyreia cuneata, Argyreia setosa, LC-HRMS, Phytochemicals, DPPH

Abstract

Objective: The present study was designed for phytochemical screening and biological activities of Argyreia cuneata (L.) and Argyreia setosa (L.) medicinal plants.

Methods: The mature leaves of A. cuneata (L.) and A. setosa (L.) were extracted with methanol (Ac-Me, As-Me) and ethyl acetate (Ac-EA, As-EA) solvent followed by qualitative and quantitative phytochemical analysis. Antimicrobial activity was evaluated by agar well diffusion method, whereas the activities such as antioxidant, antidiabetic, and anti-inflammatory were determined by in vitro methods. Liquid chromatography-high-resolution mass spectroscopy (LC-HRMS) was used to recognize the bioactive components.

Results: The highest phenolic content (0.840±0.130 mg GAE/ml extract) reported from As-Me and for flavonoids 0.128±0.012 mg QE/ml from Ac- EA. The Ac-Me exhibited higher inhibition zone against all the bacteria used for study. In antioxidant activity, Ac-Me and Ac-EA report highest 2,2 dipheny l,1 picryl hydrazine (IC50=0.580±0.012 mg/ml) and nitric oxide radical scavenging potential (IC50=0.772±0.059 mg/ml), respectively. For antidiabetic activity, As-Me showed highest α-amylase inhibition activity as well as glucose adsorption. In anti-inflammatory activity, Ac-EA exhibits highest (IC50=0.529±0.009 mg/ml) protein denaturation inhibition and Ac-Me showed highest (91.56±1.96%) HRBC hemolysis inhibition. The LC-HRMS analysis of methanolic extract reports the majority of phenolic compounds.

Conclusion: The study showed that the plants A. cuneata (L.) and A. setosa (L.) are well exploited and can be used for the source of potent natural bioactive components. This study also may thereby provide an insight in screening of crude drug.

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References

Ali SA, Hamed, MA, EL-Rigal NS, Shabana MH, Kassem MES. Chemical constituents of Argyreia speciosa Fam. Convolvulaceae and its role against Hypergycemia. Journal of Applied Pharmaceutical Science 2011; 08,76-84.

Awad R, Arnason JT, Trudeau V, Bergeron C, Budzinski JW, Foster BC, Merali Z. Phytochemical and Biological analysis of Skullcap (Scutellaria lateriflora L.): A medicinal plant with anxiolytic properties. Phytomedicine 2003; 10, 640-649.

Azami L, Shukal I, Gupta S S, Yadav NP, Kant K, Rao CV. In vitro and In vivo study of Argyreia speciosa on chronic gastric ulceraction and metabolic studies. Proceding of the National Academy of Sciences 2019; 89, 1039-1045.

Banerjee A, Maji B, Mukherjee S, Choudhuri K, Seal T. In-vitro Antidiabetic & Anti-oxidant Activities of Methanol Extract of Tinospora sinensis. Journal of Applied Biology & Biotechnology 2017; 5(3), 61-67. DOI: http://10.7324/JABB.2017.50311

Bhutkar M, Bhinge S, Randive D, Wadkar G, Todkar S. Studies on Glucose Adsorption Capacity of some Indigenous Plants. Global Journal of Pharmacy & Pharmaceutical Sciences 2018; 5(1), 555651. http://dx.doi.org/10.19080/GJPPS.2018.05.555651

Chippada SC, Volluri SS, Bammidi SR, Vangalapati M. In-vitro Anti-inflammatory Activity of Methanolic Extract of Centella asiatica by HRBC membrane Stabilisation. Rasayan Journal of Chemistry 2011; 4(2), 457-460.

Das MSC, Gayathri DS. In-vitro Glucose Binding Activity of Terminalia bellirica. Asian Journal of Pharmaceutical & Clinical Research 2015; 8(2), 320-323.

Debanjali S, Sharma P, Hasnu, S, Nath S, Akhtar S, Tanti B. Phytochemical Screening & Antioxidant Activity of Leaf Extract of Phlogacanthus thyrsiflorus Nees,- a medicinal plant of Assam, India. Bioscience Discovery 2018; 9(2), 237-243.

Dharmadeva S, Galgamuwa LS, Prasadinite C, Kumarasinghe N. In-vitro Anti-inflammatory Activity of Ficus racemose L. bark using Albumine Denaturation Method. AYU 2018; 39(4), 239-242. DOI: http://10.4103/ayu.AYU_27_18

Dina TA, Rahman MA, Ahmed NU, Uddin MA. Analgesic and anti-inflammatory properties of Argyreia argentea methanol extract in animal model. JTUSCI 2010; 3, 1-7.

Gangwar M, Gautam MK, Sharma AK, Tripathi YB, Goel RK, Nath G. Antioxidant capacity and Radical Scavenging Effect of Polyphenol Rich Mallotus philippenesis Fruit Extract on Human Erythrocytes: An In-vitro study. The Scientific World Journal 2014; 279451, http://dx.doi.org/10.1155/2014/279451

Gokhale AB, Damre, AS, Saraf MN. “Investigations into immunomodulatory activity of Argyreia speciosa”, Journal of Ethnopharmacology 2003; 84: 109-114.

Gul R, Syed UJ, Syed FSS, Jahan N. Priliminary phytochemical analysis of Alkoloids, and Antioxidant activity of crude plant extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal 2017; 5873648, https://doi.org/10.1155/2017/5873648

Gunathilake KDPP, Ranaweera KKDS, Vasantha Rupasinghe HP. In-vitro Anti-inflammatory Properties of Selected Green Leafy Vegetables. Biomedicine 2018; 6, 107. http://dx.doi.org/10.3390/biomedicines6040107

Hoang L, Benes F, Fenclova M, Kronusova O, Svarcova V, Rehorova K, Svecova EB, Vosatka M, Hajslova J, Kastanek P, Viktorova J, Ruml T. Phytochemical Composition & in-vitro Biological Activity of Iris spp. (Iridaceae): a new source of bioactive constituents for the inhibition of oral bacterial biofilm. Antibiotics 2020; 9, 403. http://dx.doi.org/10.3390/antibiotics9070403

Jaiswal SK, Rao CV, Sharma B, Mishra P, Das S, Dubey MK. Gastroprotective effect of standardized leaf extract from Argyreia speciosa on experimental gastric ulcer in rats. Journal of Ethnophrmacology 2011; 137: 341-344.

Jeet K, Thakur R. Phytochemical and Thin Layer Chromatographic profile of different extracts from whole areal part- Argyreia nervosa. International Journal of Pharmacy and Therapeutics 2012; 3(3), 237-242.

Jew-Ming Chao, Dermarderosian AH. Identification of Ergoline Alkaloids in the genus Argyreia and related genera and their chemotaxonomic implications in the Convolvulaceae. Phytochemistry 1973; 12, 2435-2440.

Jia-Yan HU, Xiu-Hong WU,Yong-Qin YIN, Jie-Tao PAN, Bang-Wei YU. Three resin glycosides isolated from Argyreia acuta, including two isomers. Chinese Journal of Natural Medicine 2018; 16, 354-357.

Khlif I, Jellali K, Michel T, Halabalaki M, Learndros A, Skaltounis, Allouche N. Characteristics, Phytochemical analysis & Biological activities of extracts from Tunisian Chetoui Olea europaea Variety. Journal of Chemistry 2015; 418731, http://dx.doi.org/10.1155/2015/418731

KIM, Jeong-Kyu, Chang-Soo KANG, Jong-Kwon LEE, Young-Ran KIM, Hye-Yun HAN, Hwa Kyung YUN. Evaluation of Repellency Effect of Two Natural Aroma MosquitoRepellent Compounds, Citronella and Citronellal. Entomological Research 2005; 35(2), 117-120.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem 1951;193, 265.

Margareth BCG, Miranda JS. Biological activities of Lupeol. International Journal of Biomedical and Pharmaceutical Sciences 2009; 3 (1), 46-66.

Nguemfo EL, Dimo T, Dongmo AB, Azebaze AGB, Alaoui K, Asongalem AE, Cherah Y, Kamtchouing P. Antioxidative and anti-inflammatory activities of some isolated constituents from the stem bark of Allanblackia monticola Staner L.C (Guttiferae). Inflammopharmacology 2009; 17, 37-41. DOI http://10.1007/s10787-008-8039-2

Osman NI, Sidik NJ, Awal A, Adam NAM, Rezali NI. In-vitro Xanthine oxidase and Albumin denaturation inhibition assay of Barringtonia racemose L. & total phenolic content analysis for potential Anti-inflammatory use in gouty arthritis. Journal of Intercultural Ethnopharmacology 2016; 5(4), 343-349. DOI: http://10.5455/jice.20160731025522

Paulke A, Kremer C, Wunder C, Toennes SW. Analysis of Lysergic acid amid in human serum and urine after ingestion of Argyreia nervosa seeds. Anal Bioanal Chem 2012; 404, 531-538.

Raghavendra HL, Prashith Kekuda TR, Akarsh S, Ranjitha MC, Ashwini HS. Phytochemical Analysis, Antimicrobial & Antioxidant activities of different parts of Pleocaulus sessilis (Nees) Bremek (Acanthaceae). International Journal of Green Pharmacy 2017; 11(2), 98-107.

Rehman G, Hamayun M, Iqbal A, Islam S, Arshad S, Zaman K, Ahmad A, Shehzad A, Hussain A, InJung Lee. In-Vitro Antidiabetic effects & Antioxidant Potential of Cassia nemophila Pods. Biomed Research International 2018; 1824790, 6. https://doi.org/10.1155/2018/1824790

Santhi K, Sengottuvel R. Qualitative & Quantitative phytochemical analysis of Moringa concanesis.Nimmo. International Journal of Current Microbiology & Applied Sciences 2016; 5(1), 633-640. http://dx.doi.org/10.20546/ijcmas.2016.501.064

Sartale GD, Sartale RG, Benelli G, Kumar G, Pugzhendhi A, Dong-Su Kim, Kan-Seung Shin. Anti-diabetic potential of silver nanoparticles synthesized with Argyreia nervosa leaf extract high synergistic antibacterial activity with standard antibiotics against foodborne bacteria. J Chust Sci 2017; 28:1709-1727.

Shukla YN, Shrivastava A, Sunil K, Sushil K. Phytotoxic and antimicrobial constituents of Argyreia speciosa and Oenothera biennis. Journal of Ethnopharmacology 1999; 67: 241-245.

Sunyong Y, Kwansoo K, Hojung N, Doheon L. Discovering Health Benefits of Phytochemicals with Integrated Analysis of the Molecular Network, Chemical Properties and Ethanopharmacological Evidence. Nutrients 2018; 10, 1042. http://dx.doi.org/10.3390/nu10081042

Tariq AL, Reyaz AL. Significances & Importance of Phytochemical Present in Terminalia chebula. International Journal of Drug Development and Research 2013; 5(3), 256-262.

Thakur J, Sharma S, Mukhija M, Kalia AN. Flavonoid fraction of Argyreia nervosa leaves with antiulcer potential in different experimental rat models. IJPRBS 2013; 2(6), 557-574.

Tofern B, Kaloga M, Witte L, Hurtmann T, Eich E. Occurrence of Loline alkaloids in Argyreia mollis (Convolulaceae). Phytochemistry 1999; 51, 1177-1180.

Troeman, DPR, Van Hout D, Kluytmans JAJW. Antimicrobial Approaches in the Prevention of Staphylococcus aureus infections: a review. Journal of Antimicrobial Chemotherapy 2019; 74, 281-294, doi:10.1093/jac/dky421.

Vieira C, Evangelista S, Cirillo R, Lippi A, Maggi CA, Manzini S. Effect of Ricinoleic acid in acute and subchronic experimental models of inflammation. Mediators of Inflammation 2000; 9, 223-228, DOI: 10.1080/09629350020025737.

Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R. Phytochemical Analysis of Medicinal Plants Occuring in Local Area of Mardan. Biochemistry and Analytical Biochemistry 2013; 2(4), 144. DOI: http://10.4172/2161-1009.1000144.

Xiaole C, Min G, Rongchao J, Weiqian DH, Xiaowen T, Yuling L, Denggao Z, Kun Z, Wenhua C, Xi Z, Zhaojun S, Panpan W. Design, synthesis and α-glucosidase inhibition study of novel embelin derivatives. Journal of Enzyme Inhibition and Medicinal Chemistry 2020; 35(1), 565-573, DOI: 10.1080/14756366.2020.1715386.