GAS CHROMATOGRAPHY - MASS SPECTROMETRY ANALYSIS OF BIOACTIVE CONSTITUENTS FROM THE MARINE STREPTOMYCES

Authors

  • Usha Nandhini S sathyabama university
  • SANGARESHWARI S
  • KUMARI LATA

Abstract

 

Objective: The objective was to study the antimicrobial activity of active eluent of thin layer chromatography (TLC) from Streptomyces isolated from
marine sample.
Methods: To determine the antimicrobial activity by agar well diffusion method. Active compounds were extracted by solvent extraction method and
purified using TLC. The presence of active compounds was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis. Identification of
active Streptomyces by 16S rRNA partial gene sequencing method.
Results: The active strain was identified as Streptomyces cacaoi strain SU2 (JF730119). The GC-MS analysis revealed that the presence of dodecane,
eicosane, cetene, diethyl phthalate, phthalic acid isobutyl nonyl ester, thieno[3,2-e] benzofuran and bis (2-ethylhexyl) phthalate.
Conclusion: The methods adopted and active metabolites extracted and purified can be fruitfully employed for obtaining novel antibiotic compounds
to treat human pathogenic bacterial and fungal infections.
Keywords: Streptomyces, GC-MS, Antimicrobial, Thin layer chromatography, Marine soil.

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Author Biography

Usha Nandhini S, sathyabama university

Biotechnology

References

Takizawa M, Colwell RR, Hill RT. Isolation and diversity of actinomycetes in the chesapeake bay. Appl Environ Microbiol 1993;59(4):997-1002.

Okazaki T, Okami Y. Studies on marine microorganisms. II. Actinomycetes in Sagami Bay and their antibiotic substances. J Antibiot (Tokyo) 1972;25(8):461-6.

Kuester E, Williams ST. Selection of media for isolation of streptomycetes. Nature 1964;202:928-9.

Lechevaliemr P, Lechevaliemr A. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970;20:435-43.

Narendhran S, Rajiv P, Vanathi P, Sivaraj R. Spectroscopic analysis of bioactive compounds from Streptomyces cavouresis kuv39: Evaluation of antioxidant and cytotoxicity activity. Int J Pharm Pharm Sci 2014;6(7):319-22.

Parthasarathi S, Sathya S, Bupesh G, Samy RD, Mohan MR, Kumar GS, et al. Isolation and characterization of antimicrobial compound from marine Streptomyces hygroscopicus BDUS 49. World J Fish Mar Sci 2012;4(3):268-77.

Sudha S, Masilamani SM. In vitro cytotoxic activity of bioactive metabolite and crude extract from a new actinomycetes Streptomyces avidinii strain SU4. Int J Pharm Pharm Sci 2013;5(3):612‑6.

Ripa FA, Nikkon F, Rahman BM, Khondkar P. In vitro antibacterial activity of bioactive metabolite and crude extract from a new Streptomyces sp. Streptomyces rajshahiensis. Int J Pharm Tech Res 2010;2(1):644-8.

Usha NS, Masilamani SM. Bioactive compounds produced by Streptomycetes strain. Int J Pharm Pharma Sci 2013;5 Suppl 1:176-8.

Sharon FB, Kalidass S, Daniel RR. Qualitative analysis of antimicrobial compound by high performance thin layer chromatography method. Asian J Pharm Clin Res 2013;6(4):117-20.

Rana S, Salam MD. Antimicrobial potential of actinomycetes isolated from soil samples of Punjab, India. J Microbiol Exp 2014;1(2):00010.

Jemimah NS, Nasimunislam N, Vaishnavi B, Mohanasrinivasan V, Subathra devi C. Isolation of soil actinomycetes inhabiting amrithi forest for the potential source of bioactive compounds. Asian J Pharm Clin Res 2012;5(3) 189-92.

Selvakumar D, Arun K, Suguna S, Kumar D, Dhevendaran K. Bioactive potential of Streptomyces against fish and shellfish pathogens. Iran J Microbiol 2010;2(3):157-64.

Sivakumar K, Sahu M, Kathiresan K. Isolation and characterization of Streptomycetes producing antibiotic from a mangrove environment. Asian J Microbiol Biotechnol Environ Sci 2005;7(3):457-64.

Published

01-03-2015

How to Cite

Nandhini S, U., SANGARESHWARI S, and KUMARI LATA. “GAS CHROMATOGRAPHY - MASS SPECTROMETRY ANALYSIS OF BIOACTIVE CONSTITUENTS FROM THE MARINE STREPTOMYCES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 2, Mar. 2015, pp. 244-6, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/4597.

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