INVESTIGATION ON THE CYTOTOXIC NATURE OF LEAF AND STEM BARK EXTRACTS OF PROSOPIS CINERARIA
Abstract
Objective: To investigate the cytotoxic activity of hydroalcoholic extracts of leaf and Stem bark of Prosopis cineraria using two human cancer cell lines
such as HeLa and MCF-7.
Methods: The cytotoxic activity was evaluated by trypan blue exclusion and MTT assay. The apoptosis influencing activity of extracts in cancer cell
lines were performed using Hoechst staining technique.
Results: Among the tested cell lines, Leaf extracts was more selective cytotoxic against HeLa and MCF-7 cell line than Stem bark extracts. In apoptotic
study, morphological changes induced in HeLa and MCF-7 cells by both extracts were evidenced by using Hoechst staining and fluorescent microscopy.
It was observed that there is marked increase in the number of MCF-7 and HeLa cells with the typical features of apoptosis, consisting in cells with
condensed and fragmented nuclei.
Conclusion: The present study proves the traditional claim of the plant in the management of cancer.
Keywords: Prosopis cineraria, 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazoliumbromide, Apoptosis, Cytotoxic activity, Leaf, Stem bark.
Downloads
References
Gaidhani SN, Singh A, Kumari S, Lavekar GS, Juvekar AS, Sen S, et al. Evaluation of some plant extracts for standardization and anticancer activity. Indian J Tradit Knowl 2013;12(4):682-7.
Anonymous. The Wealth of India, Raw Materials. Vol. 8. New Delhi: CSIR; 1969. p. 247-9.
Robertson S, Narayanan N. Isolation and characterization of secondary metabolite from Prosopis cineraria (L.) Druce for anticancer activity. World J Pharm Pharm Sci 2014;3(3):876-84.
Patel S, Gheewala N, Suthar A, Shah A. In vitro cytotoxicity activity of Solanum nigrum extract against HeLa cell line and vero cell line. Int J Pharm Pharm Sci 2009;1:38-46.
Soule HD, Vazguez J, Long A, Albert S, Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst 1973;51:1409-16.
Lillie RD. H. J. Conn’s Biological Stains. 9th ed. Baltimore: The Williams and Wilkins Company; 1977. p. 158.
Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, et al. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 1988;48(17):4827-33.
Bergan R, Kyle E, Nguyen P, Trepel J, Ingui C, Neckers L. Genistein‑stimulated adherence of prostate cancer cells is associated with the binding of focal adhesion kinase to beta-1-integrin. Clin Exp Metastasis 1996;14(4):389-98.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.