GREEN SYNTHESIS OF COPPER OXIDE NANOPARTICLE FROM PLANT EXTRACT AND ITS ANTIBACTERIAL ACTIVITY

Arushi Saloki; Sanjay J Daharwal

doi:10.22159/ajpcr.2023.v16i7.47122

Authors

Arushi Saloki Department of University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India. https://orcid.org/0000-0002-0779-5024
Sanjay J Daharwal Department of University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.

DOI:

https://doi.org/10.22159/ajpcr.2023.v16i7.47122

Keywords:

Copper oxide, nanoparticles, green synthesis, antibacterial activity, bark extract

Abstract

Objective: The present study focused on the synthesis of copper oxide nanoparticles (CuO NPs) using cinchona bark extract, its characterization, and studies on antibacterial activities.

Methods: The CuO NPs were synthesized using 1 mM CuSO₄.5H₂O with 2% (m/v) aqueous bark extract of cinchona under optimum conditions (pH = 11).

Result: The formation of CuO NPs has been confirmed first by the color change from colorless to light yellow and then to Brownish. Using a UV-Visible spectrophotometer the kinetics of the reaction was studied that showed surface plasmon resonance at 382nm. Zeta potential and particle size were calculated to be -15.2mV and, 197 nm respectively. The antibacterial activity of CuO NPs was tested against gram-positive and gram-negative culture, which shows desirable activity.

Conclusion: The outcome of the study demonstrates that cinchona bark extract serves as a reducing and stabilizing agent, transforming Cu²⁺metallic ions into CuO nanoparticles The green synthesis of the copper oxide nanoparticles (CuO NPs) using cinchona bark extract, characterization, and their antibacterial activity was successfully carried out and gram-positive (S. aureus) shows more activity compared to gram-negative (E.coli) bacteria.

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Author Biographies

Arushi Saloki, Department of University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.

Sanjay J Daharwal, Department of University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.

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