NOVEL SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF DESVENLAFAXINE IN PURE AND PHARMACEUTICAL DOSAGE FORM
Abstract
Â
Objectives: Two simple, sensitive and economic spectrophotometric methods are developed for the determination of desvenlafaxine in pure and its
pharmaceutical formulations.
Methods: The developed methods are based upon the reaction of oxidative coupling of desvenlafaxine with 3-methyl-2-benzthiazolinonehydrazone
(MBTH) (Method A) and 2, 2' bipyridyl (Method B) in the presence of ferric chloride. The colored complex produced was measured at 663 nm,
522 nm for method A and B respectively against the reagent blank. The optimum experimental parameters for the color productions are
selected.
Results: Beer's law is valid within a concentration range of 20-100 μg/ml and 5-25 μg/ml and for method A, B respectively. The percentage recoveries
were found in the range of 99.47±0.1789 for method A and 100.08±0.144 for method B. The limit of detection and limit of quantification were found
to be 0.825 μg/ml and 2.5 μg/ml respectively for method A and 0.1061 μg/ml and 0.321 μg/ml for method B respectively.
Conclusion: The developed methods are applied for the determination of desvenlafaxine in bulk and its pharmaceutical formulations without any
interference from excipients.
Keywords: Spectrophotometric, Desvenlafaxine, Oxidative coupling, 3-methyl-2-benzthiazolinone hydrazone, 2, 2- bipyridyl, FeCl3.
Downloads
References
Liebowitz MR, Yeung PP, Entsuah R. A randomized, double-blind, placebo-controlled trial of desvenlafaxine succinate in adult outpatients with major depressive disorder. J Clin Psychiatry 2007;68(11):1663-72.
Rohith T, Ananda S. Quantification of potential impurities by a stability indicating HPLC method in desvenlafaxine succinate monohydrate active pharmaceutical ingredient. Int J Res Pharm Chem 2012;2(4):947‑56.
Pingle RK, Salunkhe K. Chaudhari S. RP-HPLC method development and validation of desvenlafaxine succinate monohydrate in tablet dosage form. World J Pharm Res 2014;3(6):657-74.
Mallikarjuna R, Kishore AN, Kumar BP, Sukhen S. Method developmentand validation for the simultaneous estimation of desvenlafaxine and clonazepam in bulk & tablet formulation by RP-HPLC method. Indian J Res Pharm Biotech 2013;1(4):525-32.
Moussa BA, El-Bagary RI, Al-Eryani YA. Development and validation of a stability-indicating HPLC method for the analysis of desvenlafaxine succinate in the presence of its acidic induced degradation product in bulk and pharmaceutical preparation. J Chem Pharm Res 2011;3(5):425‑37.
Ankit S, Ujiwal S, Ashim kumar S, Dhanya BS, Seth AK. Development and validation of UV spectrophotometric method for estimation of desvenlafaxine succinate ER-tablets form. Asian J Pharm Health Sci 2011;1(3):137-41.
Abirami G, Logesh R, Vetrichelvan T. Spectrophotometric method for estimation of desvenlafaxine succinate in tablet dosage form. Res J Pharm Bio Chem Sci 2011;2(2):721-9.
Anand A, Dubey SK. New sensitive and validated spectrofluorimetric method for the estimation of desvenlafaxine succinate in bulk and in pharmaceutical formulations. World J Pharm Sci 2014;2(2):196-202.
Mansing GP, Kumar BS, Bonde CG, Gurmeet SC. UV spectrophotometric method development for the determination of desvenlafaxine succinate in tablet formulation. Bull Pharm Res 2011;1(1):40-3.
Dole MN, Amit SM, Sangeeta C, Sanjay DS. Development and validation of analytical methods for estimation of desvenlafaxine succinate in bulk and solid dosage forms by UV spectroscopy. Pharm Chem 2013;5(5):270-3.
Keval LC, Rujuta DP, Dilip GM. Absorption correction spectrophotometric method for simultaneous estimation of desvenlafaxine and clonazepam in their combined dosage form. Der Pharm Lettre 2014;6(2):61-8.
Krupa MP, Jayant BD. Development and validation of UV spectrophotometric method for simultaneous estimation of desvenlafaxine and clonazepam in tablet dosage form. Int J Pharm Res Bio-Sci 2014;3(3):87-96.
Shubhangi MP, Laxman DK, Sathish YG, Sunil RD. LC-UV and LC-MS evaluation of stress degradation behavior of desvenlafaxine. J Pharm Anal 2012;2(4):264-71.
Sowjanya K, Thejaswini JC, Gurupadayya BM, Indupriya M. Spectrophotometric determination of pregabalin using Gibb’s and MBTH reagent in pharmaceutical dosage form. Der Pharm Chem 2011;3(1):112-22.
Nagaraju P, Pasumarthy, Gopal NV, Srinivas VD, Padma SV. Spectrophotometric methods for the determination of atorvastatin calcium in pure and it’s pharmaceutical dosage forms. Asian J Res Chem 2008;1(2):64-6.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.