EVALUATION OF ANXIOLYTIC ACTIVITY OF AERIAL PARTS OF Sarcostigma kleinii Wight. & Arn.
Abstract
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Objectives: The present study was designed to evaluate the anxiolytic effect of Sarcostigma kleinii through the ethanolic extract of its aerial parts.
Methods: The study duration was five days and Swiss albino mice were subjected to dosage of 400mg/kg. The anxiolytic effect of the ethanolic
extract was evaluated in light and dark, elevated plus maze, and stair case models. Finally neurotransmitters such as serotonin (5HT) and GABA was
estimated in mice brain.
Results: The ethanolic extract was effective in inducing anxiolytic effects when compared with control group. Decreased serotonin (5HT) and
increased level of GABA was observed in ethanolic extract, when compared with control group.
Conclusion: The alkaloids, flavonoids and other chemical constituents are speculated to account for the observed pharmacological effects of the
plant extract in the experimental animal paradigms used. These findings suggest that the ethanolic extract from the aerial parts of Sarcostigma kleinii
exhibits significant anxiolytic activity.
Keywords: Sarcostigma kleinii, Anxiolytic activity, Gamma-aminobutyric acid, Serotonin.
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References
Kulkarni SK, Reddy DS. Animal behavioral models for testing antianxiety agents. Methods Find Exp Clin Pharmacol 1996;18(3):219-30.
Suresh K, Anupam S. Apigenin. The anxiolytic constituent of Tumera aphrodisiaca. Pharm Biol 2006;44:84-90.
Carlini EA. Plants and the central nervous system. Pharmacol Biochem Behav 2003;75:501-12.
Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants A Compendium of Species. Vol. 5. Hyderabad: Orient Longman; 1993. p. 76-9.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 29th ed. Pune: Nirali Prakasan; 2005. p. 225.
Mukherjee PK. Qualitative analysis of plant extract for phytochemical constituents. Pharmacognosy. 1st ed. Horizon Pharmaceutical Publishers; 2002.
Evan WC. Trease and Evans Pharmacognosy. 14th ed. India: Harcourt Brace and Company; 1998.
OECD Guideline 423, for Testing of Chemicals. Acute Oral Toxicity – Acute Toxic Class Method. December, 2001.
Ankitkumar A, Ashok M, Jyothsna V, Radhakrishna B, Gowda KP. Evaluation of anxiolytic activity of aqueous and alcoholic extracts of leaves of Crataegus oxycantha in mice. Int J Pharm Biomed Sci 2008;2(3):86-9.
Gilhotra N, Dhingra D. Possible involvement of GABAergic and nitriergic systems for antianxiety-like activity of piperine in unstressed and stressed mice. Pharmacol Rep 2014;66(5):885-91.
Baretta IP, Felizardo RA, Bimbato VF, dos Santos MG, Kassuya CA, Gasparotto Junior A, et al. Anxiolytic-like effects of acute and chronic treatment with Achillea millefolium L. extract. J Ethnopharmacol 2012;140(1):46-54.
Kavya Sree K, Vijusha M, Rajani A, Hemamalini K. Screening of behavioural, muscle co-ordination and anxiolytic activities of methanolic extract of Holoptelea integrifolia (ROXB). Int Res J Pharm 2013;4(11):90-4.
Rahman H, Muralidharan P, Anand M. Inhibition of AChE and antioxidant activities are probable mechanism of Nardostacys jatamansi DC in sleep deprived Alzheimer’s mice model. Int J Pharm Technol Res 2011;3(3):1807-16.
Curzon G, Green AR. A rapid and sensitive method for measuring 5-hydroxytryptamine and 5-hydroxyindoleacetic acid, using O-phthaldehyde and L-cysteine, is presented, enabling both compounds to be measured in small areas of rat brain. Br J Pharm 1970;39:653-5.
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