IN-VITRO ANTIOXIDANT ACTIVITY AND FREE RADICAL SCAVENGING POTENTIAL OF ROOTS OF RED SAGE
Abstract
Objective: The objective of the present work is to study the in-vitro anti-oxidant activities of petroleum ether, ethyl acetate and methanolic extracts
of Salvia splendens roots.
Methods: The extracts were studied using 1,1-diphenyl-2-picryl hydrazyl, hydrogen peroxide (H2O2), total phenolic content (TPC) and total flavonoid
content (TFC). The TPC and TFC were estimated taking gallic acid and rutin calibration curve respectably.
Results: All the extracts possess in-vitro anti-oxidant activities. However, the order of possessing activities were methanolic > ethyl acetate > petroleum
ether extracts of S. splendens roots. The TPC and TFC was highest in methanolic extract.
Conclusion: It can be concluded that S. splendens roots extracts possess anti-oxidant activities. The methanolic extract of S. splendens roots possess
highest anti-oxidant activity in-vitro.
Keywords: Salvia splendens, In-vitro anti-oxidant, 1,1-diphenyl-2-picryl hydrazyl, Ferric reducing power activity, Hydrogen peroxide scavanging, Total
phenolic content, Total flavonoid content
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REFERENCES
Simonian N.A. and Coyle J.T. Oxidative stress in neurodegenerative diseases.†Annu. Rev. Pharmacol. Toxicol. 1996; 36: 83–106.
Dhalla N.S, Temsah R.M. and Netticadan T. Role of oxidative stress in cardiovascular diseases.†J. Hypertens. 2000; 18: 655–673.
Vant Veer, P, Jansen, M.C, Klerk M. and Kok F.J. Fruits and vegetables in the prevention of cancer and cardiovascular disease.†Public Health Nutr. 2000; 3: 103–107.
Bokov A, Chaudhuri A, and Richardson A. The role of oxidative damage and stress in aging.†Mech. Ageing Dev. 2004,125:811–826.
Madamanchi N.R., Vendrov A. and Runge M.S. Oxidative stress and vascular disease.†Arterioscler. Thromb. Vasc. Biol. 2005; 25:29–38.
Bravo L. Polyphenol: Chemistry, dietary sources, metabolism, and nutritional significance.†Nutr. Rev. 1998; 56:317–333.
Heim K.E., Tagliaferro A.R. and Bobilya D.J. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships.†J. Nutr. Biochem. 2002; 13: 572–584.
Pier-Giorgio P.†Flavonoids as antioxidants.†J. Nat. Prod. 2000; 63: 1035–1042.
Rice-Evans C., Miller N. and Paganga G. Antioxidant properties of phenoilc compounds.†Trends Plant Sci. 1997; 2:152–159.
Sealbert A., Johnson J. and Saltmarsh M. Polyphenols: Antioxidants and beyondâ€. Am. J. Clin. Nutr. 2005; 81: 2155–2175.
Ross J.A. and Kasum C.M. Dietary flavonoids: Bioavailability, metabolic effects, and safetyâ€. Ann. Rev. Nutr. 2002; 22: 19–34.
Rice-Evans C.A., Miller N. and Maxwell SJ. Prospects for the use of antioxidant therapies.†Drugs. 1995; 49: 345.
Shanker,D. and Unnikrishnan,P.N.; An overview: introduction to herbal plants used in
medicine,†Amruth,2001;5(4);9-16
Gharpure,Y.H.; Drug Pharm.Ind.Highlights,2003;26(6);1-6
Bobbi,A.; Splendid salvias-14 Tender Perennials for summer and fall Bloom;†Brooklyn Botanic Garden, New York, 2003; 18(3) (www.plantdelidgts.com)
Nadkarni,K.M.; The IndianMetria Medica,1991;2; p1093-1096
The Wealth of India, A Dictionary of Indian Raw Material and Industrial Productâ€, Council of Scientific and Industrial Research, New Delhi,1998;9;p195-198
Kaplan,D.R;â€Salvia Splendens of the Lamiaceae familyâ€. Plant Biology, 2001; 107
Erv, E.; Salvia splendens, Consumer horticulturistâ€. NC State University, Florida; 2000-2003(www.Salviasplendens.com)
Kokate CK, Practical Pharmacognosyâ€. 5th Edn, Vallabh Prakasham, 107-121, 1991.
Ramya Premanath And N. Lakshmidevi, Studies On Anti-Oxidant Activity of Tinospora cardifolia (Miers)â€. Journal Of American Science. 2010; 6(10);736-743.
Neha Pandey and Dushyant Barve. Antioxidant Activity of Ethanolic Extract Of Annona Squamosa Linn Bark†International Journal Of Research In Pharmaceutical And Biomedical Sciences, 2011; 2(4);1692- 1697.
Jayanthi P. and Lalitha P. Reducing Power of the Solvent Extracts of Eichhornia crassipes (mart.) Solms†Int J Pharm Pharm Sci; 2011; 3;126-128
Deore S. L., S.S. Khadabadi, B.A. Baviskar, S. S. Khadabadi, R. A. Khangenbam, U. S. Koli, N. P. Daga, P. A. Gadbail, and P. A. Jain In vitro antioxidant activity and phenolic content of croton caudatum†International Journal Of Chem Tech Research. 2009;1(2),174-176.
Maurya S. and Singh D. Quantitative Analysis of Total Phenolic Content in Adhatoda Vasica Nees Extracts†International Journal of Pharmtech Research; 2010; 4;2403-2406,
Zou Y.P., Lu Y.H., and Wei D.Z.â€Antioxidant Activity of a Flavonoid-rich Extract of Hypericum perforatum. in vitro†j. Sci. Food Agr. 2004;52; 5032–5039.
Zengin H.W., Aktumsek A., Guler G.O., Cakmak Y.S., and Yildiztugay E. Antioxidant properties of methanolic extract and fatty acid composition of centaurea urvillei dc. Subsp., rec.†Nat. Prod.;2011;5(2);123-132
Habibur Rahman, Manjula K, Anoosha T, Nagaveni K, M. Chinna Eswaraiah and Dipankar Bardalai. In-Vitro Anti-Oxidant Activity Of Citrullus Lanatus Seed Extractsâ€.Asian j.Pharmaceutical and Clinical Research;2013;6(3);152-157
Catherine A. Rice-Evans, Nicholas J. Miller and George Paganga.Structure-antioxidant activity relationships of flavonoids and phenolic acidsâ€. Free Radical Biology and Medicine. 1996; 20(7): 933–956.
Wolf Bors and Manfred Saran. Radical Scavenging by Flavonoid Antioxidantsâ€. Free Radical Research, 1987; 2(4-6): 289-294.
Catherine A. Rice-Evans, Nicholas J. Miller and George Paganga.. Antioxidant properties of phenolic compounds. Trends in Plant Scienceâ€. 1997; 2(4): 152–159.
Kumar, S., D. Kumar and O. Prakash. Evaluation of antioxidant potential, phenolic and flavonoid content of Hibiscus tiliaceus flowersâ€. EJEAF Chem., 2008; 7: 2863-2871.
Sharififar, F.,G.Dehghan-Nudeh and M. Mirtajaldini.Major flavonoids with antioxidant activity from Teucrium poliumâ€. Food Chem., 2009; 112: 885-888.
Chidambara Murthy KN, Vanitha A, Mahadeva Swamy M, and Ravishankar GA. Antioxidant and Antimicrobial Activity of Cissus quadrangularis Lâ€.Journal of Medicinal Food, 2003; 6(2): 99-105.
Park YS, Heo BG, Ham KS, Kang SG, Park YK, Nemirovski A, Tashma Z, Gorinstein S, Leontowicz H, and Leontowicz M. Analytical determination of bioactive compounds as an indication of fruit qualityâ€. J AOAC Int. 2012 ;95(6):1725-1732.
Oboh G, Akinyemi AJ, and Ademiluyi AO. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitroâ€. Exp Toxicol Pathol. 2012;64(1-2):31-36.
Mustafa AG, Singh IN, Wang J, Carrico KM, and Hall ED. Mitochondrial protection after traumatic brain injury by scavenging lipid peroxyl radicalsâ€.J Neurochem. 2010;114(1):271-280.
Maity S, Chatterjee S, Variyar PS, Sharma A, Adhikari S, Mazumder S. Evaluation of Antioxidant Activity and Characterization of Phenolic Constituents of Phyllanthus amarus Rootâ€. J Agric Food Chem. 2013;61(14):3443-3450.
Habbu PV, Mahadevan KM, Kulkarni PV, Daulatsingh C, Veerapur VP, and Shastry RA. Adaptogenic and in vitro antioxidant activity of flavanoids and other fractions of Argyreia speciosa (Burm.f) Boj. in acute and chronic stress paradigms in rodentsâ€. Indian J Exp Biol. 2010 ;48(1):53-60.
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