ETHNOPHARMACOLOGICAL INVESTIGATION OF PLEUROTUS OSTREATUS FOR ANTI-OXIDATIVE AND ANTI-INFLAMMATORY ACTIVITY IN EXPERIMENTAL ANIMALS

LAKKARAJU CHANDANA; DARNA VRN BHIKSHAPATHI

doi:10.22159/ajpcr.2024.v17i4.49533

Authors

LAKKARAJU CHANDANA Research Scholar, Bir Tikandrajit University, Canchipur, Imphal West-795003, Manipur, India
DARNA VRN BHIKSHAPATHI Research Scholar, Bir Tikandrajit University, Canchipur, Imphal West-795003, Manipur, India

DOI:

https://doi.org/10.22159/ajpcr.2024.v17i4.49533

Keywords:

Pleurotus ostreatus, Anti-oxidant effect, Reactive oxygen species, Inflammation

Abstract

Objective: This particular study was aimed to evaluate the anti-oxidant effect of Pleurotus ostreatus using the in vitro method and also against inflammation.

Methods: A methanolic extract of P. ostreatus (MEPO) was prepared by the cold maceration technique. Different tests. In vitro anti-oxidant activity was investigated using the 1,1-diphenyl-1-picryl hydrazyl (DPPH) method. Wistar rats were chosen for the study; animals weighing 150–200 g were divided into six groups of six each (n=6). Control animals were grouped as I, group II was administered with approximately 100 μL of 1% suspension of carrageenan in saline and injected into the plantar surface of the right hind paw, group III, IV, V, and VI were given with carrageenan, followed by diclofenac sodium (150 mg/kg body weight, p.o.) and MEPO at the dose of 200, 400, and 800 mg/kg orally for 21 days. The paw volume and percentage of inhibition of the paw were measured in all animals.

Results: The IC50 values of the test extract in the DPPH free radical scavenging assay were found to be 44.02±0.09 as compared with the standard drug 35.01±0.12, which was considered significant (***p<0.001). The test extract at the dose of 400 and 800 mg/kg significantly decreased (**p<0.001) the paw volume thereby, the inflammation, the % percentage of inhibition in paw volume was compared to the positive control in the carrageenan-induced paw edema.

Conclusion: The study explored a potential source for anti-oxidants in the MEPO, which also seems to be effective in chemical-induced inflammation.

Downloads

Download data is not yet available.

References

Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol. 2015 May-Jun;47(3):292-8. doi: 10.4103/0253- 7613.157127. PMID: 26069367; PMCID: PMC4450555

Duan L, Rao X, Sigdel KR. Regulation of inflammation in autoimmune disease. J Immunol Res. 2019 Feb 28;2019:7403796. doi: 10.1155/2019/7403796. PMID: 30944837; PMCID: PMC6421792

Chen Z, Bozec A, Ramming A, Schett G. Anti-inflammatory and immune-regulatory cytokines in rheumatoid arthritis. Nat Rev Rheumatol. 2019 Jan;15(1):9-17. doi: 10.1038/s41584-018-0109-2. PMID: 30341437

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010 Jul;4(8):118-26. doi: 10.4103/0973-7847.70902. PMID: 22228951; PMCID: PMC3249911

Aruoma OI. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat Res. 2003 Feb-Mar;523-524:9-20. doi: 10.1016/s0027-5107(02)00317-2. PMID: 12628499

Sarangi I, Ghosh D, Bhutia SK, Mallick SK, Maiti TK. Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans. Int Immunopharmacol. 2006 Aug;6(8):1287-97. doi: 10.1016/j.intimp.2006.04.002, PMID: 16782541

Liu RH. Health benefits of fruits and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr. 2003;78:517S-S520.

Sullivan R, Smith JE, Rowan NJ. Medicinal mushrooms and cancer therapy: Translating a traditional practice into Western medicine. Perspect Biol Med. 2006 Spring;49(2):159-70. doi: 10.1353/ pbm.2006.0034. PMID: 16702701

Barh A, Sharma VP, Annepu SK, Kamal S, Sharma S, Bhatt P. Genetic improvement in Pleurotus (oyster mushroom): A review. 3 Biotech. 2019 Sep;9(9):322. doi: 10.1007/s13205-019-1854-x, 2019 Aug 6. PMID: 31406644; PMCID: PMC6684725

Akcay C, Ceylan F, Arslan R. Production of oyster mushroom (Pleurotus ostreatus) from some waste lignocellulosic materials and FTIR characterization of structural changes. Sci Rep. 2023 Aug 9;13(1):12897. doi: 10.1038/s41598-023-40200-x. PMID: 37558821; PMCID: PMC10412599

Arawwawala M, Thabrew I, Arambewela L, Handunnetti S. Anti-inflammatory activity of Trichosanthes cucumerina Linn. in rats. J Ethnopharmacol. 2010 Oct 5;131(3):538-43. doi: 10.1016/j. jep.2010.07.028. PMID: 20654707

Domínguez M, Avila JG, Nieto A, Céspedes CL. Anti-inflammatory activity of Penstemon gentianoides and Penstemon campanulatus. Pharm Biol. 2011 Feb;49(2):118-24. doi: 10.3109/13880209.2010.503708. Epub 2010 Oct 28. PMID: 20979542

NagalingamS, Sasikumar CS, CherianKM. Extraction and preliminary phytochemical screening of active compounds in Morinda citrifolia fruit. Asian J Pharm Clin Res. 2012;5(2):179-81.

Hinneburg I, Damien Dorman HJ, Hiltunen R. Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem. 2006;97:122-9.

Garg D, Shaikh A, Muley A, Marar T. In vitro antioxidant activity and phytochemical analysis in extracts of Hibiscus rosasinensis stem and leaves. Free Radic Antioxid. 2012;2(3):41-6. doi: 10.5530/ax.2012.3.6

Rajesh KD, Vasantha S, Panneerselvam A, Valsala Rajesh NV, Jeyathilakan N. Phytochemical analysis, in vitro antioxidant potential and gas chromatography-mass spectrometry studies of Dicranopteris linearis. Asian J Pharm Clin Res. 2016;9(2):1-6. doi: 10.22159/ ajpcr.2016.v9s2.13636.14

Kennedy GL Jr. Acute toxicity studies with oxamyl. Fundam Appl Toxicol. 1986 Apr 1;6(3):423-9. doi: 10.1016/0272-0590(86)90215-0, PMID 3699328

Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and antiinflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci. 2010;2:146-55.

Reshma, Arun KP, Brindha P. In vitro anti-inflammatory, antioxidant and nephroprotective studies on leaves of Aegle marmelos and Ocimum sanctum. Asian J Pharm Clin Res. 2014;7:121-9.

Iqbal Z, Iqbal MS, Mishra K. Screening of antioxidant property in medicinal plants belonging to the family Apocynaceae. Asian J Pharm Clin Res. 2017;10:415-8.

Anoop MV, Bindu AR. In-vitro anti-inflammatory activity studies on Syzygium zeylanicum (L.) DC leaves. Int J Pharm Res Rev. 2015;4:18-27.

Leelaprakash G, Caroline RJ, Mohan DS. In vitro anti-inflammatory activity of Momordica charantia by inhibition of lipoxygenase enzyme. Int J Pharm Pharm Sci. 2012;4:148-52.

Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009 Nov-Dec;2(5):270-8. doi: 10.4161/oxim.2.5.9498. PMID: 20716914; PMCID: PMC2835915

Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutat Res. 2005;579:200-13.

Kaur N, Kishore K. Antioxidant activity of methanolic extract of Phaseolus trilobus root powder. Int J Pharm Pharm Sci. 2012;4:271-5.

Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Smith PT, et al. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. J Agric Food Chem. 2011;59:12361-7.

Talhouk RS, Karam C, Fostok S, El-Jouni W, Barbour EK. Anti-inflammatory bioactivities in plant extracts. J Med Food. 2007;10:1-0.

Wang C, Levis GB, Lee EB, Levis WR, Lee DW, Kim BS, et al. Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-[alpha] in activated RAW 264.7 cells. Int J Immunopharmacol. 2004;4:1039-49.