TO EVALUATE THE INFLAMMATORY MARKERS IN TYPE 2 DIABETES MELLITUS AND COMPARED IT WITH NORMAL HEALTHY CONTROLS

JASWANT KAUR; SHWETA; MOHIT SHARMA; SEEMA; MOHAMMED NADEEM SHAIKH

doi:10.22159/ajpcr.2024.v17i5.50194

Authors

JASWANT KAUR Department of Biochemistry, Dr. S.S. Tantia Medical College, Hospital and Research Centre, Sriganganagar, Rajasthan, India
SHWETA Department of Physiology, NC Medical College and Hospital, Israna, Panipat, India
MOHIT SHARMA Medical Officer, 7th Battalion Punjab Armed Police, Jalandhar Cantonment, Punjab, India.
SEEMA Department of Physiology, Dr. S.S. Tantia Medical College, Hospital & Research Centre, Sriganganagar, Rajasthan, India.
MOHAMMED NADEEM SHAIKH Department of Biochemistry, Dr. S.S. Tantia Medical College, Hospital and Research Centre, Sriganganagar, Rajasthan, India

DOI:

https://doi.org/10.22159/ajpcr.2024.v17i5.50194

Keywords:

Cytokines, Type 2 diabetes mellitus, Inflammation, BMI, IL-6, TNF-α, CRP

Abstract

Objective :To evaluate the serum levels of Interleukin -6, Tissue Necrosis factor – α, C- reactive Protein ( IL-6, TNF- α , CRP) in type 2 diabetes mellitus in North Population

Method: A total of 200 participants were selected in this study among them 100 were recruited as Cases who were suffering from T2DM and 100 were healthy controls. Biochemical parameters were analyzed by fully autoanalyser. Serum levels of inflammatory cytokines, TNF-α ,CRP and IL-6 were estimated by ELISA . Data was analyzed using statistical ‘software SPSS 15. TheP<0.05 were considered as statistically significant.

Result: in our study we observed that serum levels of candidate cytokines, TNF-α, IL-6 and CRP were highly significant (p<0.001) in T2DM as compared to healthy control. Fasting blood sugar, HbA1c and lipid profile parameters were recorded highly significant (p<0.001) in T2DM as compared to normal healthy controls.

Conclusion: Inflammatory cytokines play a significant role in the pathogenesis of T2DM. Hence, these cytokines can be used as early prediction biomarkers which may be helpful in the reduction of mortality rate of T2DM.

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References

Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract. 2019;157:107843. doi: 10.1016/j.diabres.2019.107843, PMID 31518657

Khan RM, Chua ZJ, Tan JC, Yang Y, Liao Z, Zhao Y. From pre-diabetes to diabetes: Diagnosis, treatments and translational research. Med (Kaunas Lith). 2019;55(9):546. doi: 10.3390/medicina55090546, PMID 31470636

Donath MY, Shoelson SE. Type 2 diabetes as an inflammatory disease. Nat Rev Immunol. 2011;11(2):98-107. doi: 10.1038/nri2925, PMID 21233852

Smitka K, Marešová D. Adipose tissue as an endocrine organ: An update on pro-inflammatory and anti-inflammatory microenvironment. Prague Med Rep. 2015;116(2):87-111. doi: 10.14712/23362936.2015.49, PMID 26093665

Pradhan AD, Cook NR, Buring JE, Manson JE, Ridker PM. CRP is independently associated with fasting insulin in nondiabetic women. Atheroscler Thromb Vasc Biol. 2003;23(4):650-5. doi: 10.1161/01. ATV.0000065636.15310.9C

Ali M. Adipose tissue as an endocrine and paracrine organ. Int J Obes Relat Metab Disord. 1998;22:1145-58.

Rehman K, Akash MS, Liaqat A, Kamal S, Qadir MI, Rasul A. Role of interleukin-6 in the development of insulin resistance and type 2 diabetes mellitus. Crit Rev Eukaryot Gene Expr. 2017;27(3):229-36. doi: 10.1615/CritRevEukaryotGeneExpr.2017019712, PMID 29199608

Rotter V, Nagaev I, Smith U. IL-6 induces insulin resistance in 3T3-L, adipocytes and is like IL-8, TNF-α, over-expressed in human fat cells from insulin resistant subjects. J Biol Chem. 2021;278:457777-84.

Wang X, Bao W, Liu J, Ouyang YY, Wang D, Rong S, et al. Inflammatory markers and risk of type 2 diabetes: A systematic review and meta-analysis. Diabetes Care. 2013;36(1):166-75. doi: 10.2337/dc12-0702, PMID 23264288

Inshah D, Sabhiya M, Fouzia R, Rabia F, Jasiya Q, Rouf M, et al. Combinatorial effect of leptin, tumor necrosis factor-α-alpha, andvitamin D intype 2 diabetes in Kashmiri population. Asian J Pharm Clin Res. 2018;10(11):477-82.

Bhat MA, Bhat SA, Ahmad SB, Qureshi W, Majid S, Ali A. Biochemical profile and genetic polymorphism of MTHFRC677T in risk of type 2 diabetes mellitus. Int J Diabetes Endocrinol. 2017;2(2):19-25.

Ali A, Ayaz A, Dar MA, Singh N, Bhat SA. A key role of insulin in diabetes mellitus. Int J Sci Res Sci. 2017;3(6):80-5.

Singh PS, Sharma H, Zafar KS, Singh PK, Yadav SK, Gautam RK, et al. Prevalence of type 2 diabetes mellitus in rural population of India- a study from Western Uttar Pradesh. Int J Res Med Sci. 2017;5(4):1363- 7. doi: 10.18203/2320-6012.ijrms20171227

VinodMahato R, Gyawali P, Raut PP, Regmi P. Association between glycemic control and serum lipid profile in type 2 diabetic patients: Glycated hemoglobin as a dual biomarker. Biomed Res. 2011;22(3):375-80.

Singh U. Prevalence of diabetes and other health-related problems across India and worldwide: An overview. J Nat Appl Sci. 2016;8(1):500-5. doi: 10.31018/jans.v8i1.825

Bertoni AG, Burke GL, Owusu JA, Carnethon MR, Vaidya D, Barr RG, et al. Inflammation, and the incidence of type 2 diabetes: The Multi-Ethnic Study of Atherosclerosis (MESA). Diabetes Care. 2010;33(4):804-10. doi: 10.2337/dc09-1679, PMID 20097779

Samuel ND, Denis DY, Ellis OD, Dark SN, Yar D, Owasu D, et al. Variations in levels of IL-6 and TNF-α in type 2 diabetes mellitus between rural and urban Ashanti Region of Ghana. BMC Endocrinol Disord. 2015;15:50.

Phosat C, Panprathip P, Chumpathat N, Prangthip P, Chantratita N, Soonthornworasiri N, et al. Elevated C-reactive protein, interleukin 6, tumor necrosis factor - alpha and glycemic load associated with type 2 diabetes mellitus in rural Thais: A cross-sectional study. BMC Endocr Disord. 2017;17(1):44. doi: 10.1186/s12902-017-0189-z, PMID 28716139