IN VITRO ASSESSMENT OF ETHANOLIC EXTRACT OF CUCUMIS MADERASPATANUS LEAVES AS AN α-AMYLASE AND α-GLUCOSIDASE INHIBITOR

NADHIYA K; MAHALAKSHMI P; KALAIVANI P

doi:10.22159/ajpcr.2024v17i11.52075

Authors

NADHIYA K Department of Biochemistry, Annai Violet Arts and Science College, Chennai, Tamil Nadu, India
MAHALAKSHMI P Department of Biochemistry, Annai Violet Arts and Science College, Chennai, Tamil Nadu, India.
KALAIVANI P Department of Biochemistry, Annai Violet Arts and Science College, Chennai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2024v17i11.52075

Keywords:

Cucumis maderaspatanus, EECM- Ethanolic leaf Extracts of Cucumis maderaspatanus and DPPH-2,2-diphenyl-1-picrylhydrazyl

Abstract

Objective: The current study was planned to study the phytochemicals, Antioxidant, and inhibitor activity of alpha-glucosidase and alpha-amylase from leaf extract of Cucumis maderaspatanus.

Methods: Extract was extracted using a soxhlet apparatus using solvents, such as n-hexane, chloroform, ethyl acetate, ethanol, and aqueous. The extracts were evaporated using a rotary evaporator. The phytochemicals were measured qualitatively and quantitatively and the antioxidant activity was done and the IC50 value was calculated.

Result: The qualitative analysis of the ethnolic leaf extract of C. maderaspatanus shows the presence of carbohydrates, terpenoids, phenol compounds, tannins, saponins, flavonoids, alkaloids, anthocyanins, betacyanins, quinones, glycosides, sterol, and coumarins. Quantitative analysis of ethanolic leaf extract of C. maderaspatanus showed that the presence of phenols (98.63±0.03), flavonoids (80.35±0.78), saponin (16.56±0.04), alkaloids (12.56±0.05), and total antioxidant (130.18±2.45). The IC50 value of various parameters such as DPPH (IC50=265), Nitric oxide (IC50=213), Hydrogen peroxide (IC50=355), Hydroxyl (IC50=290), Superoxide (IC50=145), alpha-amylase activity (IC50=48), and alpha-glucosidase activity (IC50=76).

Conclusion: From the study, it was concluded that the ethanolic leaf extracts of C. maderaspatanus have good antioxidant capacity, alpha-amylase, and alpha-glucosidase inhibitor activity due to phytochemicals present in it.

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References

Nadhiya K, Haripriya D, Vijayalakshmi K. Pharmacognostic and preliminary phytochemical analysis on Benincasa hispida fruit. Asian J Pharm Clin Res. 2014;7(1):99-101.

Nair SS, Kavrekar V, Mishra A. In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol. 2013;3(1):128-32.

Mohammed A, Adelaiye AB, Bakari AG Mabrouk MA. Anti-diabetic and some haematological effects of ethylacetate and N-butanol fractions of Ganoderma lucidum aqueous extract in alloxan-induced diabetic wistar rats. Int J Med Med Sci. 2009;1(12):530-5.

Settu S, Arunachalam S. Comparison of phytochemical analysis and In-vitro pharmacological activities of most commonly available medicinal plants belonging to the Cucurbitaceae family. Res J Pharm Technol. 2019;12(4):1541-6.

Gomathy G, Vijay T, Sarumathy K, Gunasekaran S, Palani S. Phytochemical screening and GC-MS analysis of Mukia maderaspatana (L.) leaves. J Pharm Sci. 2012;2(12):104-6.

Petrus AJ. Ethnobotanical and pharmacological profile with propagation strategies of Mukia maderaspatana (L.) M. Roem.- A concise overview. Indian J Nat Prod Resour. 2013;4(1):9-26.

Rahman AH, Anisuzzaman M, Alam MZ, Islam AK, Zaman AT. Taxonomic studies of the cucurbits grown in the Northern parts of Bangladesh. Res J Agric Biol Sci. 2006;2(6):299-302.

Sofowara A. Medicinal Plants and Tradional Medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd.; 1993. p. 191-289.

Trease GE, Evans WC. Pharmacognosy. Brailliae Tiridal Can. 11th ed., Vol. 11. London: Macmillan Publisher’s; 1989. p. 45-50.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall Ltd.; 1973. p. 47-188.

Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J Agric Food Chem. 2002;50:1619-24.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10:178-82.

Han Chien LI, Yang BY, Chun-Han KO, Murase Y. Quantification for saponin from a soapberry (Sapindus mukorossi Gaertn) in cleaning products by a chromatographic and two colorimetric assays. J Fac Agric

Kyushu Univ. 2009;54(1):215-21.

Koleva II, Van Beek TA, Linssen JP, De Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Anal. 2002;13:8-17.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr. 1986;44:307-15.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and (15N) nitrate in biological fluids. Anal Biochem. 1982;126:131-8.

Liu F, Ooi VE, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 1997;60:763-77.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen. 1989;10:1003-8.

Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: A simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 1987;165:215-9.

Preito P, Pinedo M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269:337-41.

Rege AA, Chowdhary AS. Research article evaluation of alpha-amylase and alphaglucosidase inhibitory activities of Ocimum sanctum Linn. Int J Pharm Sci Rev Res. 2014;25:130-3.

Walker JM, Winder JS, Kellam SJ. High-throughput micro titer plate-based chromogenic assays for glycosidase inhibitors. Appl Biochem Biotech. 1995;38:141-6.

Kim YK, Nam AK, Kurihara H, Kim MS. Potent a-glucosidase inhibitors purified from the red algae Grateloupia elliptica. Phytochemistry. 2008;60:2820-5.

You Q, Chen F, Wang X, Luo PG, Jiang Y. Inhibitory effects of muscadine anthocyanins on α-glucosidase and pancreatic lipase activities. J Agric Food Chem. 2011;59:9506-11.

Nadhiya K, Vijayalakshmi K. Evaluation of total phenol, flavonoid contents and in vitro antioxidant activity of Benincasa hispida fruit extracts. Int J Pharm Chem Biol Sci. 2014;4(2):332-8.

Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40:945-8.

Arbaayah HH, Umi KY. Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and split gill mushroom (Schizophyllum commune) ethanolic extracts. Mycosphere. 2013;4:661-73.

Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem. 1995;43:1813-9.

Poongunran J, Perera HK, Fernando WI, Jayasinghe L, Sivakanesan R. “α-Glucosidase and α-amylase inhibitory activities of nine Sri Lankan antidiabetic plants. J Pharm Res Int. 2015;7(5):365-74