PROTECTIVE EFFECT OF COMBINATION OF ETHANOLIC EXTRACTS OF XIMENIA AMERICANA AND TERMINALIA MACROPTERA AGAINST ALCOHOL-INDUCED HEPATOTOXICITY IN RATS

Mahamadou BALLO; Sekou BAH; ESTELLE NH YOUL

doi:10.22159/ajpcr.2024v17i11.52539

Authors

Mahamadou BALLO Faculté de Pharmacie, Université des Sciences, des Techniques et des Technologies de Bamako, Mali
Sekou BAH Faculté de Pharmacie, Université des Sciences, des Techniques et des Technologies de Bamako, Mali
ESTELLE NH YOUL Laboratoire du Développement du Médicament, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.

DOI:

https://doi.org/10.22159/ajpcr.2024v17i11.52539

Keywords:

Antioxidant, Hepatoprotective, Ximenia americana, Terminalia macroptera, Combination

Abstract

Objective: The aim of this study was to evaluate the hepatoprotective effect of the combination.

Methods: Serum liver markers, tissue antioxidant activity, and histological changes in the livers of rats from the blank, negative (distilled water), positive (silymarin 100 mg/kg bw), and test (combination 500 mg/kg bw) groups were measured after 7 days of pretreatment and induction of hepatotoxicity by 10 g/kg bw alcohol every 12 h for 48 h.

Results: Rats in the negative control group showed a highly significant (p<0.001) increase in aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (BT) levels by 281.13%, 221.7%, and 93.44%, respectively, compared to rats in the blank group. Pretreatment with the combination resulted in a highly significant (p<0.001) decrease in AST, ALT, and BT levels of 69.19%, 62.24%, and 41.52%, respectively. The study of tissue oxidative stress parameters revealed a very significant (p<0.01) increase in superoxide dismutase (123.08%), glutathione (131.66%), and catalase (49.01%) activities and a significant (p<0.05) decrease in malondialdehyde concentration (59.72%) in the group pretreated with the combination compared with the negative control group. Steatosis and necrosis estimated at 50% were observed in rats in the negative control group. In contrast, necrosis observed in the group pre-treated with the combination was <10%.

Conclusion: These data suggest that the combination is effective in preventing the elevation of biochemical markers and the imbalance of enzymatic and non-enzymatic antioxidant systems caused by alcohol.

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